LOCUS BG064353 646 bp mRNA linear EST 12-MAY-2010 DEFINITION H3018F01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3018F01 3', mRNA sequence. ACCESSION BG064353 VERSION BG064353.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 646) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064353.1. Other_ESTs: H3018F01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3018 row: F column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..646 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3018F01-3" /db_xref="taxon:10090" /clone="H3018F01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 182 c 179 g 138 t ORIGIN 1 cagtggttcc agatttaatg gaaggtgcgt agggtgctgc actgcaccct gaggctgagt 61 tgctgcaccg ccaggtatgg agcgtctgct ttttgcaact gaacagagga acgaatggag 121 catataacta acaagaaaga aggccctcct gcctgcctct gagacctggg ggatcaggtc 181 acctctgacc ccctgagcct gggggcctcc ccatccccac tgcccttctg aagtcaattc 241 ctccagaagg agcctggcta ggacagcccc gaataggggc aaacagggag cgggatgctc 301 agagcccctc ccagtcattt taggaacaat aaatactgtg tgacagtagc aggaagggca 361 tccgtgggct gtgtggcagc cagagcaggc tggccaggtg cctgccctca ttctatgcgc 421 aggatgatat ggatgcccga gtccgtgaac acgggcccac tcatctcccc tgtccgtaga 481 gcaaacgacg catcctcaaa tggtttctgc atctgacctc tgctgaaggg acccaggtct 541 cccctggctt tggcagagct gcaatcactg aactgtgagg ccagagattc aaagtcttcc 601 tctcctgact taatcttctg gatatagcca ttgatgagct ccaggg //