LOCUS BG064347 673 bp mRNA linear EST 12-MAY-2010 DEFINITION H3018E06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3018E06 3', mRNA sequence. ACCESSION BG064347 VERSION BG064347.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 673) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064347.1. Other_ESTs: H3018E06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3018 row: E column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..673 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3018E06-3" /db_xref="taxon:10090" /clone="H3018E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 165 a 174 c 182 g 152 t ORIGIN 1 ggattttctt tttttagtaa aacattcttt tattaaaaga acaagtgctg tttacgaact 61 gcccttcgta caaataacat ccgttataca aagatacaag acccaggcta tgcacaattc 121 caggcttgga ggtcgcaggg gaacaccgtc tcttgggctg aggatataaa ggtttcagaa 181 agaatgaaaa atgagcccct gggtttgcaa tctgtggctt cccttccttg ctcccttagg 241 aagggtctgc tacatggaaa caggctggga tggaaggaag ggagccacag tccttcgggt 301 cttcagtccc aagtggggct ggagaaagtg aacacaggag aaagaggacg gtcctcagtc 361 cccaacgctt cagtaggtca agacaaggtg gtctgctggg aactagacac acctgtaatc 421 caggaggaag cggctggagg gaacagaggg actccctgac cccacctccc tccgtcctag 481 cgggcacctc tccctggccc cacctccctc tgtcctagtg ggcacctctc cccaggcacc 541 agcagtctgt tactcccttt ggccctctgc actcttggga tgactgagac tagcagggcg 601 ggcctgtgtc tctgaaaaca ggtcagcatt tccagggtgg gaagaggaaa tggaactggt 661 agcctctttg ggc //