LOCUS BG064322 624 bp mRNA linear EST 12-MAY-2010 DEFINITION H3018C03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3018C03 3', mRNA sequence. ACCESSION BG064322 VERSION BG064322.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 624) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064322.1. Other_ESTs: H3018C03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3018 row: C column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..624 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3018C03-3" /db_xref="taxon:10090" /clone="H3018C03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 129 a 167 c 186 g 142 t ORIGIN 1 gggcatggaa aattctttat tgtgacactg ggcctaggtg gcagcaggtc atcgaggcac 61 tttgaggaag ggttcgtgta gtacttcaaa gagcctgcgg gccttctgtg ggcccaggcc 121 cgggcataag gctagatcct cccttgatgc ggtgaagagc tgttccaggg atccaaatgt 181 agccaggagg gtctggctgt cggtcttgtt cacagatttc acggtggtca gacactcagt 241 ggcccgtgat aggaagttct gctccagctt ttccataagg aggtcggctg gcttctgctc 301 atacgccttg taggtctcca ggtaccgccc tgcttcctct gcactccagg ccaggaccag 361 ggtgcagtca gccaagatgc acatcttagc cagctccttg agagcctgct ggggatcttt 421 cacatccact tgaaccagca gcacacgaag ggcgaagttc ttccccaggc tctgcagccg 481 ttcatggatg tagtctggat ggaggttgtg gtagcggagg ctgaggaaaa gggcgcaggt 541 gctctggccc agcacataat cgggaatcac ctcaccgaat tcccagggca cattgcgcac 601 aaacttcaac acggggttgc ccct //