LOCUS BG064264 571 bp mRNA linear EST 26-JAN-2001 DEFINITION H3017E09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3017E09 3', mRNA sequence. ACCESSION BG064264 VERSION BG064264.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 571) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3017E09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3017 row: E column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..571 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3017E09-3" /db_xref="taxon:10090" /clone="H3017E09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 153 a 135 c 131 g 152 t ORIGIN 1 gagataaggc ctcactgtag cccaggctag ccttgaactt gctatgtggt tgaagtcatc 61 cttgaacttc tgatcatcct gtccctccat cccaagtgct gagattacag gcatgtgtca 121 caggtcactt ctacgtggtg ttggggatcc aacccagggt tgcatgcatg ctagtcaaat 181 actctaccaa ctgggctaca cccccagcct taaagattgc ccttttaata actgacattt 241 gattctacta tgtctgctgt ggcttattaa atgaatggct ataactaagc aaaggaagaa 301 gactgaaacc cccaaaaaac agagttaatt aacattcgga accttcttta aaaggcaagc 361 tacaagatga tgcccagaat gatggagagg gagaaccatg ggggctggat gcagcgggtg 421 cctagaccga tggcaacgtc aggcctggct ggcacttgcc tatctagttc cttaacaact 481 tgggcctcgg atcagtattt taagatggtg gtacagatct ctatttataa gctcagctct 541 gccatgaaaa ctcttgatgc ttgccttgct c //