LOCUS BG064139 723 bp mRNA linear EST 26-JAN-2001
DEFINITION H3015H10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3015H10 3', mRNA sequence.
ACCESSION BG064139
VERSION BG064139.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 723)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3015H10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3015 row: H column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..723
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3015H10-3"
/db_xref="taxon:10090"
/clone="H3015H10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 177 a 186 c 162 g 198 t
ORIGIN
1 ccaatgccaa tttattttaa tcgggctctg tgttatctat gctttgcctc tttcctttat
61 aatcgtacaa cctttgaaaa ttacataagt tgtaatgatc taatactatt aatagccatt
121 caggaaaaaa aagttccctc ccgcccaaca accatccagg ggaaatgtaa agtcctcatt
181 agaggccagc tcaacgcggc ctctaccctg gtagaaataa aaatcaaaaa gatgaggaga
241 aaacaaatcg gctaagaggt gtgttgccag tgtctttcag gagtggggcc ctggctgttg
301 cctgggtggt gaaaggcaga gcctacagcc tgcaggaggg cagccgggcg gcctcacagc
361 tacatcttcc tcaccccggc acagccacag cccagtttag gtgtcgtgaa aatcctttag
421 cagggttctc ctccgctgct ctgctatgtg catctggttt tctaagtccc ctctgtcgta
481 gctgtctgca cgctcaactt tccatgaaag gttttcgatc tcagcctcca gctgagcctg
541 ctggtattca aacagcttct tcctgggacc ggattccatg tagtatgcat atggataggt
601 gtactgcagg gtatatcgac acttggctaa gagcttggca gcattctgta ggtactgcca
661 gtcaatccaa gttcccaggt tgttcataac cctctcctga atcttctcgt gaatccgctc
721 ata
//