LOCUS BG064084 794 bp mRNA linear EST 12-MAY-2010
DEFINITION H3015C11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3015C11 3', mRNA sequence.
ACCESSION BG064084
VERSION BG064084.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 794)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064084.1.
Other_ESTs: H3015C11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3015 row: C column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..794
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3015C11-3"
/db_xref="taxon:10090"
/clone="H3015C11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 253 a 172 c 158 g 211 t
ORIGIN
1 aggtacttct atgttcaaat gaacataatt ccatttgcaa aatgtaattg ctaatgtctt
61 ttccataaaa catggttggc aaaataaaaa aggcacagaa gggactaagt gggtaggttt
121 tcaactttca gagaagggta ccaaagcaca tgtcaaatat cgttcatgaa gaaaagctga
181 aaaataatat attcggtaat tcatgcacaa cttgagctac gcaaaacttt ttagaagtct
241 cctgaggctg gaggagaaaa tgagttttcc aatcacatgc gcctgtgaac agaataaagc
301 tacacataca ttcggtttta acagtggacc catgtttttc agaacaaaat gtgaggcaat
361 taagcaaagg aaaacaatac caaaatacct tcataaacaa acttgacctg tagcctctgt
421 cttgtgcgga gaatgtccaa taagatgtaa aatgcagcat acatagtcat gtaatctaga
481 aactttccat gataatttat tgcaaattgc acttgacagt tcaccacaac aatggaaagc
541 tggccccctg ttggtttgca cagtccttga gccccaaact gaagttgaag tcaccagtaa
601 aatgatctgg ataaaaagtt tgctgctgtg ctcaaccagc tggctccacc ttcagggtgc
661 gatccaaccc gggacactgc cttgtcctgc tcctttgtgg gactctcatc ctcaggcagg
721 tagtcaggta gctctgtgtt ctcttccact tccacagggg catcctgaaa agggacagca
781 tctcttctcc actc
//