LOCUS BG063632 720 bp mRNA linear EST 12-MAY-2010 DEFINITION H3009F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3009F05 3', mRNA sequence. ACCESSION BG063632 VERSION BG063632.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 720) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063632.1. Other_ESTs: H3009F05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3009 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..720 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3009F05-3" /db_xref="taxon:10090" /clone="H3009F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 179 a 195 c 188 g 158 t ORIGIN 1 ctctcgaagc actttattag ccaatatatc tcttcaaagg gctcacttgg ggctgtgcaa 61 gaacccagcc tgataggtgt ccccatctcc aggtcatgcc agcgacaagc caacagcctg 121 cgtaggtggc tggagctaag ccagaaggag ccgaaatccg aggaccttca cctagacatc 181 ctggggctcc gctaaagacc ctcatgctcc tgtgtggaaa gatcctttgg cagccacaac 241 cgagaggact agagggagcc cagtgctcct gcaaagcaga tcttctgttc tgccttcagc 301 ctcaagttga agtccttggg tgcctcgaag atgagcatga tgtggagctc aggttgaact 361 cgcccagggg ctcacccttg tgcatgggaa tgccctcctt gttacaaagc tcaggtcatt 421 gtagtacctt gctgtacctg gggctgtttg tgtgcaagtc ctaggaacaa gaaggttgag 481 cctgtcctca actctgccca gcccaggtgg cagagacaaa ggcctaacta atcaacactc 541 ccagtatcag ctgtgacttt gacaaggagg ggcaggggtt agacataaat gctgaaaggc 601 tagtgccagc agcagccatt aaaacctaaa aaggcagagt gggtacagat tttctcaccc 661 aagccaactc taccctcctg cagggctcat ccttgcttct gatcagggat ctggcagaag //