LOCUS BG063590 744 bp mRNA linear EST 12-MAY-2010 DEFINITION H3009B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3009B04 3', mRNA sequence. ACCESSION BG063590 VERSION BG063590.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 744) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063590.1. Other_ESTs: H3009B04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3009 row: B column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..744 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3009B04-3" /db_xref="taxon:10090" /clone="H3009B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 212 a 191 c 155 g 186 t ORIGIN 1 cacttttata attttattgt tttttttttc tcttcacatc ttttaaaata gaaatagcct 61 atgaacaaca gtttcttctc ccaaaacctc aattcaattg attgcctcag aaaggctgtc 121 agaacatcac ttggaggcac aggtcagtgg cacacttaat gcctttgccc tggggtcaat 181 ccccaacact ggagagggga gagacaaaaa caaaaaacta caaatcaaaa cttaaacact 241 gccatctcac tccctgcaca actactggac tcagcaggca gcaagttcaa atttctgccc 301 acaaaaatca gctggtcagt actaaatctg ttgtcagcca gacttctctc tgctcgtctc 361 tttgcagttc ttccatcagc gaatcccagg ctgatgggca tctttcaggg cacagtgaaa 421 ttagaaggag cagtagtcag tcaggttcta gtcacctctc ctccaccagc tggccagctg 481 ggaaactatc tccagcctcg ctcccggctt tatcttcttc ttccttaaga cattgctcgc 541 agttgcacag aacaatgact acagtgtcca ttaacctgag aagtggtttg agcctgacat 601 ctttcctgag ctaaaaatga gaaaaagcag agctacggcc ttcctgccac agggtcaaga 661 gagggaaagc tctgaagggc agcgctggga agcaatgaac tctcctcaag acgagggaca 721 agagggatgt gctgcagcca aggg //