LOCUS BG063422 650 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007B01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007B01 3', mRNA sequence. ACCESSION BG063422 VERSION BG063422.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 650) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063422.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3007 row: B column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..650 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007B01-3" /db_xref="taxon:10090" /clone="H3007B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 184 a 177 c 159 g 130 t ORIGIN 1 atctgcatgt ccccagggca cagcccagga tgggcattta atacaggctg gtgtgtgcag 61 aaccaaccac acagacatcc aacagcagcc tcattagata ctgaacccaa accaatactg 121 ccagcaagag aaggtcacta gtaattacac caaggtgaaa atgaccggcg ctgacaggca 181 tttttcaacg tccaaaggga caacccgtta cagatctttg cagcaggtta gtctaaagct 241 aactcactgc agccagccaa ctgcaggcag ctcaggacag aggtaagtgc agtcatgtcc 301 tcaggagagg agaccaagtc ctgactcctg acagagggat aagccctcaa tctctgggcc 361 agaagccaac agacttagca catcagaggt gacaccatac caaaggccaa gaggagacct 421 ctaggagggt agcctcctgc ctctggcttc cttcctgtcc tgcatggggt tttctaacct 481 tccacccagg cagtgaggca cgaactgtga acgaggggag ccgctgggtt ggtagtaaat 541 gctgtctccc tctctacaca tgttttactg cagtccataa aacagaacat ggtgttagag 601 cgctgtgcat cataagcacc caacaaaacc tagctgtgct agggtctgct //