LOCUS BG063381 611 bp mRNA linear EST 12-MAY-2010 DEFINITION H3006F02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3006F02 3', mRNA sequence. ACCESSION BG063381 VERSION BG063381.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 611) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063381.1. Other_ESTs: H3006F02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3006 row: F column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..611 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3006F02-3" /db_xref="taxon:10090" /clone="H3006F02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 126 a 169 c 143 g 173 t ORIGIN 1 ggagacaggg tttctctgta tagctctggc tgtcctggaa ctcactctgt agaccaggct 61 ggccttgaat acagaaatcc gcttgcctct gcctcaaagt gctgggatta aaggcatgtg 121 ccaccactgc ccagctggtt ttgttttctc aagacaagat ttctctatca gccctggctg 181 tcctggaact tgctctgtag atcaggctag ccttgaatcc acagagatca gtcttctgcc 241 ccctgagtgc tggaatcaaa gccatgtgcc agcacctcca gggcagagat gatgtttcta 301 atagtcttcc ttactgaaat ctgctgaggt aaggtatgca ctgacgtgtg tgtgtgtgtg 361 tgtgtgtgtg tgtgtgtgtc acagcatttc tttttccttt tgagacaggg tctcctgtag 421 ctcaggctgg cctcactagc ctcgaccttc tgatccttct gcctctacct tctgaatgtg 481 agaattacag gcatgcacca tcatgcccac tttaatgtct gccccagaac ccagagaccc 541 atgcatgcta ggcaagtact ccccacccca ccctggcctc agttctgagc atcagtactg 601 agtactcagc c //