LOCUS BG063204 605 bp mRNA linear EST 12-MAY-2010 DEFINITION H3004B08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3004B08 3', mRNA sequence. ACCESSION BG063204 VERSION BG063204.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 605) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063204.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3004 row: B column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..605 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3004B08-3" /db_xref="taxon:10090" /clone="H3004B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 172 c 185 g 110 t ORIGIN 1 gtctctgaac cgcggggtcg tgtttgtgtt tgacccgcgg gcgctggtgg cgtggcacgg 61 ctgaagccgg tgccagcggg gcggggcccg gcgcagcgga ggcggaggaa gacggagcgg 121 gagtccgggc aggcccggcg gcgccatgga actgggcccg gagccccccc accggcgccg 181 cctgttcttc gcttgcagcc ccacgcctgc gccgcagccc acggggaaga tgctgtttgg 241 cgcgtcagct gctggcggac tgtcccctgt caccaacctg acggtcacca tggaccagct 301 ggaaggactg ggcagtgact gtgagaaaat ggaagtgaga aataacagca gtctacagag 361 aatgggctcc tcagaatcga ccgattcagg tttctgtcta gattctcctg ggcccttgga 421 cagtaaagaa aaccttgaaa tttccctgac gagaataaat tccctacctc agaagctcct 481 gggatgtagc ccagccctga agaggagcca ctctgattct ctagaccacg acacctttca 541 cctcatcgac caggatgaaa ataaagaaaa tgaagcattt gaatttaaaa agccaatacg 601 acctg //