LOCUS BG063050 774 bp mRNA linear EST 12-MAY-2010
DEFINITION H3001E04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3001E04 3', mRNA sequence.
ACCESSION BG063050
VERSION BG063050.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 774)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG063050.1.
Other_ESTs: H3001E04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3001 row: E column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..774
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3001E04-3"
/db_xref="taxon:10090"
/clone="H3001E04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 203 a 181 c 164 g 226 t
ORIGIN
1 atttacattt atataattta tattttgaat ttatattatg cgcaatatta ttttttaatt
61 tatttattca ttatatgtaa gtatactgta gctgtcctca aatactccag aagaaagagg
121 gcatcagatt tcgttacaga tggttgtgag ccaccatgtg gttgctggga tttgaactcg
181 ggaccctcgg aagagcagtc agcgctctta accactgagc catctcgcca gccccacaat
241 attattatta ttattattat tattattgta ttggtgttct gcttaaatgt atatctgttc
301 accatgtgtg tatggtgaca gcagagacta gaagaagatg ttatatcccc tggaactgga
361 cttacagact gttgtgaact ttcatgttgg ttccaagaat gaactcaggt cctctagaag
421 agtagccaac gttcttaact gctgagccct ccctctggct ggcatctcag cttttcctgt
481 cgacgctggg atgggatacg aactcgggtc ctcatgctta cacggcaagt tctttactgc
541 ctggaccacc tcccagcctt gtcactgatc ctctctatcc agcaatggag aagaagggtc
601 ccagcaacag caaaccctga gctagaccat tcccaaagac ttctccatct catgtctttg
661 caagacagga actcccatca agccttgaca gaagtcacag caggctaccc cccagcattt
721 gggaggcaga ggcagaggca gaggcaggca ggtctctgtc aatgcaaggt catc
//