LOCUS BG063048 680 bp mRNA linear EST 12-MAY-2010 DEFINITION H3001E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3001E02 3', mRNA sequence. ACCESSION BG063048 VERSION BG063048.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 680) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG063048.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3001 row: E column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..680 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3001E02-3" /db_xref="taxon:10090" /clone="H3001E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 192 a 184 c 151 g 153 t ORIGIN 1 gcagtcaaag gtcagccact ttaattcagg gacatgaatc atttatcagt caaaaatgtg 61 tagaggcaga ggcaacaaca acaacaacaa caacaacaac gacaacaaca acagaagaac 121 caaaccccaa taaaataacc ctaaaacaga ccctgacaaa accaaagctc atgtctctga 181 actactccct ttagcagcaa tccctcaggt gaactgacca gggagccagc tgacattccc 241 atttcctaat gccttcattt tggagcctct gtgggaccct tgcctttcgg atacatctca 301 gatttggggg agaaagaagt cctttggaaa caaatctgaa gccaggtttc actgccatgg 361 cccctgcaga aggacccttg ccattttaca tcctccttcc aaggttaaaa ggcttaaagt 421 ccacggcctg tcttcttaac gttccccgag gggtccaggt gcttggctgt gactctagag 481 gacactgtga agctggatgc agtgaccaca agggctggag actcacagag gctctggaag 541 atggaaattc ctatggcttt ggagtgagga caaactgcct ctgcctgccc ttactggtcc 601 tcatcagtac actggtagga cagccagcct aggggcttgg ttccttaccc tgccccagca 661 gaggctttct gatgcctttc //