LOCUS BG063032 732 bp mRNA linear EST 26-JAN-2001
DEFINITION H3001C06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3001C06 3', mRNA sequence.
ACCESSION BG063032
VERSION BG063032.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 732)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3001 row: C column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..732
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3001C06-3"
/db_xref="taxon:10090"
/clone="H3001C06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 197 a 186 c 165 g 182 t
ORIGIN
1 cgcgttgggc tatttattaa caacatgagc aaagggcttc aaagactggt ggatccaggc
61 cgcttctttt tcttccttct ttggataaca cctattgtct ggtccaaatc tgggaacttc
121 cgattggcag ctgactccaa ataggcccaa agttcatctg tctctgaatc ttcaaatgga
181 atttggttgg gctctagagg ttcttaaggg ctcatcctcc cagttatccc ggcctgacag
241 gagaaagcag ttcagataag acttaaaata aacctaattc tctagcccac gtgaatagcg
301 gatactggga atcctcaaga ctctgtccaa gccacatgag gctaataggg gctttgaagg
361 gtaacactgc agcccacaaa aactacctgc aagccccttc aaacagccca atagataccc
421 ttaccgaaaa tcaatttctg ccagctgccc atgagtgttc cctacgatga cggaattgcc
481 ctcaggagta agggtcatag ctgtcagtgg gtactctcca taggaggcct ctaggactgg
541 cacaaagagn ataaagnact ggatcataga cacggacctg gtggtaccct gtgcatgtga
601 caagtttctg tgacccaggg atgaactgtg tatcttggtc ccaaatagga actcggagat
661 caagccaatc attccgcaca tttgtggccc tgaatacagg ctcctcagac ccctgtgagt
721 cccatacttt ca
//