LOCUS BG062950 524 bp mRNA linear EST 12-MAY-2010 DEFINITION H3002D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002D03 3', mRNA sequence. ACCESSION BG062950 VERSION BG062950.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 524) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG062950.1. Other_ESTs: H3002D03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3002 row: D column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..524 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3002D03-3" /db_xref="taxon:10090" /clone="H3002D03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 115 c 121 g 150 t ORIGIN 1 agtgttgcag tggagactcc cttgaggtct cccatttatt taagtttata tgctatggtt 61 agagaagtaa cttcattgag ctattatata tgggtatgcc caactatgac cttattagat 121 cccttacgaa ttcaggagtc taatattcac aacatggtca ctgcctttta gtttcttata 181 caaacatgcc cccctttccc ccttttaaag atatgtgcct ggagagagtg ggaaaaaaaa 241 aaccaaccca aaacatcaag ggcttgctag aaagatgaag cctagaaaag ataaaatccc 301 cgtggcagtt ctgctcagcg gcttgctgtc tgtgagcgga ggcacagtgc tcacgtaggg 361 cagcactctg cccagtggtt tcttggtcag gatggtgtga aagcccttga ggttttctgt 421 gtttggtggg aggaactgga aatcccattt actggatcca ctcgatgttc aggcaccgtg 481 ctatgaacgc aaacatatcc gagacttctt ctatcacttt ggcg //