LOCUS BC146997 990 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens amylase, alpha 2A (pancreatic), mRNA (cDNA clone
MGC:182100 IMAGE:9056925), complete cds.
ACCESSION BC146997
VERSION BC146997.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 990)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 990)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JUN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 13 Row: B Column: 6
single stranded.
FEATURES Location/Qualifiers
source 1..990
/db_xref="H-InvDB:HIT000503243"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:182100 IMAGE:9056925"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_362"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: TGTTAGTC"
gene 1..990
/gene="AMY2A"
/gene_synonym="AMY2B"
/gene_synonym="PA"
/db_xref="GeneID:279"
/db_xref="HGNC:HGNC:477"
/db_xref="MIM:104650"
CDS 110..907
/gene="AMY2A"
/gene_synonym="AMY2B"
/gene_synonym="PA"
/codon_start=1
/product="amylase, alpha 2A (pancreatic)"
/protein_id="AAI46998.1"
/db_xref="GeneID:279"
/db_xref="HGNC:HGNC:477"
/db_xref="MIM:104650"
/translation="MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECE
RYLAPKGFGGVQVSPPNENVAIYNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
NVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
GDIENYNDATQVRDCRLTGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMW
PGDIKAILDKLHNLNSNWFPAGSKPFIYQEVHQYLYAYKISSYSLEN"
BASE COUNT 302 a 170 c 214 g 304 t
ORIGIN
1 aacagtttgc cctcaagtat ttattcatgc taatatttac tttgtaaaat gtgcttctta
61 caggaatata aatagtttct ggaaaggaca ctgacaactt caaagcaaaa tgaagttctt
121 tctgttgctt ttcaccattg ggttctgctg ggctcagtat tccccaaata cacaacaagg
181 acggacatct attgttcatc tgtttgaatg gcgatgggtt gatattgctc ttgaatgtga
241 gcgatattta gctccgaagg gatttggagg ggttcaggtc tctccaccaa atgaaaatgt
301 tgcaatttac aaccctttca gaccttggtg ggaaagatac caaccagtta gctataaatt
361 atgcacaaga tctggaaatg aagatgaatt tagaaacatg gtgactagat gtaacaatgt
421 tggggttcgt atttatgtgg atgctgtaat taatcatatg tgtggtaacg ctgtgagtgc
481 aggaacaagc agtacctgtg gaagttactt caaccctgga agtagggact ttccagcagt
541 cccatattct ggatgggatt tcaatgatgg taaatgtaaa actggaagtg gagatatcga
601 gaactacaat gatgctactc aggtcagaga ttgtcgtctg actggtcttc ttgatcttgc
661 actggagaag gattacgtgc gttctaagat tgccgaatat atgaaccatc tcattgacat
721 tggtgttgca gggttcagac ttgatgcttc caagcacatg tggcctggag acataaaggc
781 aattttggac aaactgcata atctaaacag taactggttc cctgcaggaa gtaaaccttt
841 catttaccag gaggtacatc aatacttata tgcctataaa atatcatctt attcgttaga
901 aaattaatgg aagatttaat taaaaatgca atttctgtag gataaggaat gagacattta
961 cataaaacag tgttctttaa cctcctcttc
//