LOCUS BC146839 5507 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens solute carrier family 12 (sodium/potassium/chloride
transporters), member 2, mRNA (cDNA clone MGC:181942
IMAGE:9056767), complete cds.
ACCESSION BC146839
VERSION BC146839.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5507)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5507)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JUN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Ambion
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 684 Row: c Column: 5
single stranded.
FEATURES Location/Qualifiers
source 1..5507
/db_xref="H-InvDB:HIT000503099"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:181942 IMAGE:9056767"
/tissue_type="Testicle, PCR rescued clones"
/clone_lib="NIH_MGC_382"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: ACCGCTTC"
gene 1..5507
/gene="SLC12A2"
/gene_synonym="BSC"
/gene_synonym="BSC2"
/gene_synonym="NKCC1"
/db_xref="GeneID:6558"
/db_xref="HGNC:HGNC:10911"
/db_xref="MIM:600840"
CDS 108..3698
/gene="SLC12A2"
/gene_synonym="BSC"
/gene_synonym="BSC2"
/gene_synonym="NKCC1"
/codon_start=1
/product="SLC12A2 protein"
/protein_id="AAI46840.1"
/db_xref="GeneID:6558"
/db_xref="HGNC:HGNC:10911"
/db_xref="MIM:600840"
/translation="MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVSSVPE
DAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAA
AAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAA
GVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTM
DAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYT
AESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTIT
GLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVV
ELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFV
IGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANIS
GDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVYDTIVTELTNCTSA
ACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPK
IFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFF
LASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYV
IVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAP
NSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYA
PVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQ
YGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKP
ITHKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTK
KKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAF
EEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSST
ANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS"
BASE COUNT 1544 a 1094 c 1253 g 1616 t
ORIGIN
1 tctctcacct ggtcttgcgg ctgtggccac cgccggccag gggtgtggag ggcgtgctgc
61 cggagacgtc cgccgggctc tgcagttccg ccgggggtcg ggcagctatg gagccgcggc
121 ccacggcgcc ctcctccggc gccccgggac tggccggggt cggggagacg ccgtcagccg
181 ctgcgctggc cgcagccagg gtggaactgc ccggcacggc tgtgtcctcg gtgccggagg
241 atgctgcgcc cgcgagccgg gacggcggcg gggtccgcga tgagggcccc gcggcggccg
301 gggacgggct gggcagaccc ttggggccca ccccgagcca gagccgtttc caggtggacc
361 tggtttccga gaacgccggg cgggccgctg ctgcggcggc ggcggcggcg gcggcagcgg
421 cggcggctgg tgctggggcg ggggccaagc agacccccgc ggacggggaa gccagcggcg
481 agagcgagcc ggctaaaggc agcgaggaag ccaagggccg cttccgcgtg aacttcgtgg
541 acccagctgc ctcctcgtcg gctgaagaca gcctgtcaga tgctgccggg gtcggagtcg
601 acgggcccaa cgtgagcttc cagaacggcg gggacacggt gctgagcgag ggcagcagcc
661 tgcactccgg cggcggcggc ggcagtgggc accaccagca ctactattat gatacccaca
721 ccaacaccta ctacctgcgc accttcggcc acaacaccat ggacgctgtg cccaggatcg
781 atcactaccg gcacacagcc gcgcagctgg gcgagaagct gctccggcct agcctggcgg
841 agctccacga cgagctggaa aaggaacctt ttgaggatgg ctttgcaaat ggggaagaaa
901 gtactccaac cagagatgct gtggtcacgt atactgcaga aagtaaagga gtcgtgaagt
961 ttggctggat caagggtgta ttagtacgtt gtatgttaaa catttggggt gtgatgcttt
1021 tcattagatt gtcatggatt gtgggtcaag ctggaatagg tctatcagtc cttgtaataa
1081 tgatggccac tgttgtgaca actatcacag gattgtctac ttcagcaata gcaactaatg
1141 gatttgtaag aggaggagga gcatattatt taatatctag aagtctaggg ccagaatttg
1201 gtggtgcaat tggtctaatc ttcgcctttg ccaacgctgt tgcagttgct atgtatgtgg
1261 ttggatttgc agaaaccgtg gtggagttgc ttaaggagca ttccatactt atgatagatg
1321 aaatcaatga tatccgaatt attggagcca ttacagtcgt gattctttta ggtatctcag
1381 tagctggaat ggagtgggaa gcaaaagctc agattgttct tttggtgatc ctacttcttg
1441 ctattggtga tttcgtcata ggaacattta tcccactgga gagcaagaag ccaaaagggt
1501 tttttggtta taaatctgaa atatttaatg agaactttgg gcccgatttt cgagaggaag
1561 agactttctt ttctgtattt gccatctttt ttcctgctgc aactggtatt ctggctggag
1621 caaatatctc aggtgatctt gcagatcctc agtcagccat acccaaagga acactcctag
1681 ccattttaat tactacattg gtttacgtag gaattgcagt atctgtaggt tcttgtgttg
1741 ttcgagatgc cactggaaac gtttatgaca ctatcgtaac agagctaaca aactgtactt
1801 ctgcagcctg caaattaaac tttgattttt catcttgtga aagcagtcct tgttcctatg
1861 gcctaatgaa caacttccag gtaatgagta tggtgtcagg atttacacca ctaatttctg
1921 caggtatatt ttcagccact ctttcttcag cattagcatc cctagtgagt gctcccaaaa
1981 tatttcaggc tctatgtaag gacaacatct acccagcttt ccagatgttt gctaaaggtt
2041 atgggaaaaa taatgaacct cttcgtggct acatcttaac attcttaatt gcacttggat
2101 tcatcttaat tgctgaactg aatgttattg caccaattat ctcaaacttc ttccttgcat
2161 catatgcatt gatcaatttt tcagtattcc atgcatcact tgcaaaatct ccaggatggc
2221 gtcctgcatt caaatactac aacatgtgga tatcacttct tggagcaatt ctttgttgca
2281 tagtaatgtt cgtcattaac tggtgggctg cattgctaac atatgtgata gtccttgggc
2341 tgtatattta tgttacctac aaaaaaccag atgtgaattg gggatcctct acacaagccc
2401 tgacttacct gaatgcactg cagcattcaa ttcgtctttc tggagtggaa gaccacgtga
2461 aaaactttag gccacagtgt cttgttatga caggtgctcc aaactcacgt ccagctttac
2521 ttcatcttgt tcatgatttc acaaaaaatg ttggtttgat gatctgtggc catgtacata
2581 tgggtcctcg aagacaagcc atgaaagaga tgtccatcga tcaagccaaa tatcagcgat
2641 ggcttattaa gaacaaaatg aaggcatttt atgctccagt acatgcagat gacttgagag
2701 aaggtgcaca gtatttgatg caggctgctg gtcttggtcg tatgaagcca aacacacttg
2761 tccttggatt taagaaagat tggttgcaag cagatatgag ggatgtggat atgtatataa
2821 acttatttca tgatgctttt gacatacaat atggagtagt ggttattcgc ctaaaagaag
2881 gtctggatat atctcatctt caaggacaag aagaattatt gtcatcacaa gagaaatctc
2941 ctggcaccaa ggatgtggta gtaagtgtgg aatatagtaa aaagtccgat ttagatactt
3001 ccaaaccact cagtgaaaaa ccaattacac acaaagaatc caaaggccct attgtgcctt
3061 taaatgtagc tgaccaaaag cttcttgaag ctagtacaca gtttcagaaa aaacaaggaa
3121 agaatactat tgatgtctgg tggctttttg atgatggagg tttgacctta ttgatacctt
3181 accttctgac gaccaagaaa aaatggaaag actgtaagat cagagtattc attggtggaa
3241 agataaacag aatagaccat gaccggagag cgatggctac tttgcttagc aagttccgga
3301 tagacttttc tgatatcatg gttctaggag atatcaatac caaaccaaag aaagaaaata
3361 ttatagcttt tgaggaaatc attgagccat acagacttca tgaagatgat aaagagcaag
3421 atattgcaga taaaatgaaa gaagatgaac catggcgaat aacagataat gagcttgaac
3481 tttataagac caagacatac cggcagatca ggttaaatga gttattaaag gaacattcaa
3541 gcacagctaa tattattgtc atgagtctcc cagttgcacg aaaaggtgct gtgtctagtg
3601 ctctctacat ggcatggtta gaagctctat ctaaggacct accaccaatc ctcctagttc
3661 gtgggaatca tcagagtgtc cttaccttct attcataaat gttctataca gtggacagcc
3721 ctccagaatg gtacttcagt gcctagtgta gtaactgaaa tcttcaatga cacattaaca
3781 tcacaatggc gaatggtgac ttttctttca cgatttcatt aatttgaaag cacacaggaa
3841 agttgctcca ttgataacgt gtatggagac ttcggtttta gtcaattcca tatctcaatc
3901 ttaatggtga ttcttctttg ttgaactgaa gtttgtgaga gtagttttcc tttgctactt
3961 gaatagcaat aaaagcgtgt taactttttg attgatgaaa gaagtacaaa aagcctttag
4021 ccttgaggtg ccttctgaaa ttaaccaaat ttcatccata tatcctcttt tataaactta
4081 tagaatgtca aactttgcct tcaactgttt ttatttctag tctcttccac tttaaaacaa
4141 aatgaacact gcttgtcttc ttccattgac catttagtgt tgagtactgt atgtgttttg
4201 ttaattctat aaaggtatct gttagatatt aaaggtgaga attagggcag gttaatcaaa
4261 aatggggaag gggaaatggt aaccaaaaag taaccccatg gtaaggttta tatgagtata
4321 tgtgaatata gagctaggaa aaaaagcccc cccaaatacc tttttaaccc ctctgattgg
4381 ctattattac tatatttatt attatttatt gaaaccttag ggaagattga agattcatcc
4441 catacttcta tataccatgc ttaaaaatca cgtcattctt taaacaaaaa tactcaagat
4501 catttatatt tatttggaga gaaaactgtc ctaatttaga atttccctca aatctgaggg
4561 acttttaaga aatgctaaca gatttttctg gaggaaattt agacaaaaca atgtcattta
4621 gtagaatatt tcagtattta agtggaattt cagtatactg tactatcctt tataagtcat
4681 taaaataatg tttcatcaaa tggttaaatg gaccactggt ttcttagaga aatgttttta
4741 ggcttaattc attcaattgt caagtacact tagtcttaat acactcaggt ttgaacagat
4801 tattctgaat attaaaattt aatccattct taatatttta aaacttttgt taagaaaaac
4861 tgccagtttg tgcttttgaa atgtctgttt tgacatcata gtctagtaaa attttgacag
4921 tgcatatgta ctgttactaa aagctttata tgaaattatt aatgtgaagt ttttcattta
4981 taattcaagg aaggatttcc tgaaaacatt tcaagggatt tatgtctaca tatttgtgtg
5041 tgtgtgtgta tatatatgta atatgcatac acagatgcat atgtgtatat ataatgaaat
5101 ttatgttgct ggtattttgc attttaaagt gatcaagatt cattaggcaa actttggttt
5161 aagtaaacat atgttcaaaa tcagattaac agatacaggt ttcatagaga acaaaggtga
5221 tcatttgaag ggcatgctgt aatttcacac aattttccag ttcaaaaatg gagaatactt
5281 cgcctaaaat actgttaagt gggttaattg atacaagttt ctgtggtgga aaatttatgc
5341 aggttttcac gaatcctttt tttttttttt ttttttttga gacggagtct cgctctgttg
5401 ccacgctgga atgcagtaac gtgatctcgg ctcactgcga cctccacctc cccagttcaa
5461 gcgattctcc tgcctcagcc tccctagtag ctgggactac gggtgca
//