LOCUS BC146772 5617 bp mRNA linear HUM 12-JUL-2007
DEFINITION Homo sapiens roundabout, axon guidance receptor, homolog 2
(Drosophila), mRNA (cDNA clone MGC:166849 IMAGE:9007219), complete
cds.
ACCESSION BC146772
VERSION BC146772.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5617)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5617)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (16-JUN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Kazusa DNA Research Inst.
cDNA Library Preparation: Kazusa DNA Research Institute
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCF Plate: 9 Row: b Column: 9.
FEATURES Location/Qualifiers
source 1..5617
/db_xref="H-InvDB:HIT000435564"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:166849 IMAGE:9007219"
/tissue_type="Clones donated by Kazusa DNA Research Inst."
/clone_lib="Kazusa-KIAA-pBluescriptIISK+"
/lab_host="DH5alpha T1-resistant"
/note="Vector: pBluescript II SK+"
gene 1..5617
/gene="ROBO2"
/gene_synonym="SAX3"
/db_xref="GeneID:6092"
/db_xref="HGNC:HGNC:10250"
/db_xref="MIM:602431"
CDS 7..4143
/gene="ROBO2"
/gene_synonym="SAX3"
/codon_start=1
/product="roundabout, axon guidance receptor, homolog 2
(Drosophila)"
/protein_id="AAI46773.1"
/db_xref="GeneID:6092"
/db_xref="HGNC:HGNC:10250"
/db_xref="MIM:602431"
/translation="MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSK
GEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSK
PDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGH
PEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAE
LTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDY
TLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFP
CETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQ
ALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVIS
WLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTES
GATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSN
SWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQG
VDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATS
SWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPP
QSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRS
VIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVV
KQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLL
NAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGA
IYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDL
MGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELE
HYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFG
QQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYV
SGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQR
PRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSK
PSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL
"
BASE COUNT 1758 a 1206 c 1264 g 1389 t
ORIGIN
1 gtcaaaatga gtctgctgat gtttacacaa ctactgctct gtggattttt atatgttcgg
61 gttgatggat cgcgtcttcg ccaggaggac tttcccccgc ggattgtgga gcatccttcc
121 gatgtcatcg tctctaaggg cgagcccacg actctgaact gcaaggcgga gggccggcca
181 acgcccacca ttgagtggta caaagatggg gagcgagtgg agactgacaa ggacgatccc
241 cggtcccaca ggatgcttct gcccagcgga tccttattct tcttgcgcat cgtgcacggg
301 cgcaggagta aacctgatga aggaagctac gtttgtgttg cgaggaacta tcttggtgaa
361 gcagtgagtc gaaatgcgtc tctggaagtg gcattgttac gagatgactt ccgacaaaac
421 cccacagatg ttgtagtggc agctggagag cctgcaatcc tggagtgcca gcctccccgg
481 ggacacccag aacccaccat ctactggaaa aaagacaaag ttcgaattga tgacaaggaa
541 gaaagaataa gtatccgtgg tggaaaactg atgatctcca ataccaggaa aagtgatgca
601 gggatgtata cttgtgttgg taccaatatg gtgggagaaa gggacagtga cccagcagag
661 ctgactgtct ttgaacgacc cacatttctc aggaggccaa ttaaccaggt ggtactggag
721 gaagaagctg tagaatttcg ttgtcaagtc caaggagatc ctcaaccaac tgtgaggtgg
781 aaaaaggatg atgcagactt gccaagagga aggtatgaca tcaaagacga ttacacacta
841 agaattaaaa agaccatgag tacagatgaa ggcacctata tgtgtattgc tgagaatcgg
901 gttggaaaaa tggaagcctc tgctacactc accgtccgag ctcccccaca gtttgtggtt
961 cggccaagag atcagattgt tgctcaaggt cgaacagtga catttccctg tgaaactaaa
1021 ggaaacccac agccagctgt tttttggcag aaagaaggca gccagaacct acttttccca
1081 aaccaacccc agcagcccaa cagtagatgc tcagtgtcac caactggaga cctcacaatc
1141 accaacattc aacgttccga cgcgggttac tacatctgcc aggctttaac tgtggcagga
1201 agcattttag caaaagctca actggaggtt actgatgttt tgacagatag acctccacct
1261 ataattctac aaggcccagc caaccaaacg ctggcagtgg atggtacagc gttactgaaa
1321 tgtaaagcca ctggtgatcc tcttcctgta attagctggt taaaggaggg atttactttt
1381 ccgggtagag atccaagagc aacaattcaa gagcaaggca cactgcagat taagaattta
1441 cggatttctg atactggcac ttatacttgt gtggctacaa gttcaagtgg agagacttcc
1501 tggagtgcag tgctggatgt gacagagtct ggagcaacaa tcagtaaaaa ctatgattta
1561 agtgacctgc cagggccacc atccaaaccg caggtcactg atgttactaa gaacagtgtc
1621 accttgtcct ggcagccagg tacccctgga acccttccag caagtgcata tatcattgag
1681 gctttcagcc aatcagtgag caacagctgg cagaccgtgg caaaccatgt aaagaccacc
1741 ctctatactg taagaggact gcggcccaat acaatctact tattcatggt cagagcgatc
1801 aacccccaag gtctcagtga cccaagtccc atgtcagatc ctgtgcgcac acaagatatc
1861 agcccaccag cacaaggagt ggaccacagg caagtgcaga aagagctagg agatgtcctt
1921 gtccgtcttc ataatccagt tgtgctgact cccaccacgg ttcaggtcac atggacggtt
1981 gatcgccaac cccagtttat ccaaggctac cgagtgatgt atcgtcagac ttcaggtctg
2041 caggcgacat cttcgtggca gaatttagat gccaaagtcc cgactgaacg aagtgctgtc
2101 ttagtcaacc tgaaaaaggg ggtgacttat gaaattaaag tacggccata ttttaatgag
2161 ttccaaggaa tggatagtga atctaaaacg gttcgtacta ctgaagaagc cccaagtgcc
2221 ccaccacagt ctgtcactgt actgacagtt ggaagctaca atagcacaag tattagtgtt
2281 tcctgggatc ctcctcctcc agatcaccag aatggaatta tccaagaata caagatctgg
2341 tgtctaggaa atgaaacgcg attccatatc aacaaaactg tggatgcagc cattcggtcc
2401 gtaataattg gtggattatt cccaggtatt caataccggg tagaggttgc agctagtacc
2461 agtgcagggg ttggagtaaa gagtgagcca cagccaataa taatcgggag acgcaatgaa
2521 gttgtcatta ctgaaaacaa taacagcata actgagcaaa tcactgatgt ggtgaagcaa
2581 ccagccttta tagctggtat tggtggtgcc tgctgggtaa ttctgatggg ttttagcata
2641 tggttgtatt ggcgaagaaa gaagaggaag ggactcagta attatgctgt tacgtttcaa
2701 agaggagatg gaggactaat gagcaatgga agccgtccag gtcttctcaa tgctggtgat
2761 cccagctatc catggcttgc tgattcttgg ccagccacga gcttgccagt aaataatagc
2821 aacagtggcc caaatgagat tggaaatttt ggccgtggag atgtgctgcc accagttcca
2881 ggccaagggg ataaaacagc aacgatgctc tcagatggag ccatttatag tagcattgac
2941 ttcactacca aaaccagtta caacagttcc agccaaataa cacaggctac cccatatgcc
3001 acgacacaga tcttgcattc caacagcata catgaattgg ctgtcgatct gcctgatcca
3061 caatggaaaa gctcaattca gcaaaaaaca gatctgatgg gatttggtta ttctctacct
3121 gatcagaaca aaggtaacaa tggtgggaaa ggtggaaaaa agaagaaaaa taaaaactct
3181 tctaaaccac agaaaaacaa tggatccact tgggccaatg tccctctacc tcccccccca
3241 gtccagcccc ttcctggcac ggagctggaa cactatgcag tggaacaaca agaaaatggc
3301 tatgacagtg atagctggtg cccaccattg ccagtacaaa cttacttaca ccaaggtctg
3361 gaagatgaac tggaagaaga tgatgatagg gtcccaacac ctcctgttcg aggcgtggct
3421 tcttctcctg ctatctcctt tggacagcag tccactgcaa ctcttactcc atccccacgg
3481 gaagagatgc aacccatgct gcaggctcac ctggatgagt tgacaagagc ctatcagttt
3541 gatatagcaa aacaaacatg gcacattcaa agcaataatc aacctccaca gcctccagtt
3601 ccaccgttag gttatgtgtc tggagccttg atttctgatt tggaaacgga tgttgcagat
3661 gatgatgccg acgacgaaga ggaagcttta gaaatcccca ggcccctgag agcactggac
3721 cagactcctg gatccagcat ggacaatcta gacagctctg tgacaggaaa agcctttacc
3781 tcctctcaaa gacctcgacc taccagccca ttttctactg acagtaacac cagtgcagcc
3841 ctgagtcaaa gtcagaggcc tcggcccact aaaaaacaca agggagggcg gatggaccaa
3901 caaccagcat tgcctcatcg aagggaagga atgacagatg aggaggcctt ggtgccctat
3961 agcaagccca gtttcccatc tccaggtggc cacagctcat caggaacagc ttcttctaag
4021 ggatccactg gacctaggaa aaccgaggtg ttgagagcag gccaccagcg caatgccagc
4081 gaccttcttg acataggata tatgggctcc aacagtcaag gacagtttac aggtgaatta
4141 tagtaaatga gaggagacat acaaagctgc tctgaaggac catcaggtcc ggactcatgg
4201 aagtgatgac tctaaacagt gcaatgaaca atttatttat gtactattaa aagaactgta
4261 aatgcaatgt aaagacacac agccacacat atcccacaga tattttcatt gtgttcttct
4321 cttaagtaca ccacccacct taactctttc ttgtcaggag tatataaaaa agaaagaaaa
4381 caaaactcgc cctacaggaa gaaaaggatt ctcctctgta tataatttct tttgtgcatt
4441 gctatgcaag ctcactcttt ttagctctgc tcatattatt gtctgttctt attggtctgt
4501 tgtactatat gtgaattaat aggctgtggt gccatatatt aacttttaat tgtgtaactt
4561 ttatgtttaa attttgcact gcaattttat ttggtgataa gcacaaatct ctactcctca
4621 tgacatgaag aaaaagactg aatgtgaagg gagtttctgt actgtaagct agattggata
4681 atgatggctg taacaaatca tgttagatgg ttttcagttg gggtgtagaa ataggaagat
4741 gcaaaggaac aatggtgttg gcaaagtctt ctttgaatat cagggactga gtcaataaaa
4801 aaaatagtag aaaggtggct tttactattg acaaaagccg gggtcaaaaa aagtagttta
4861 agtcttaaga ctgaatatgc attaaagtat gcaggtagca aagatgtaat aaatttgctt
4921 aaaaaaagaa attaaagttt tatttagaat caattttacc tgtcattgta attgacccat
4981 ctgagaatta caataagcaa gaggaaatta aggtgttttg caagagctgt atttatatta
5041 cagtttttta aaaacatttt ctgaattatc gtaattaagc tctccaactc gttaagtcag
5101 aatataatat gaagttcccc aaggaaacga acaaaatgaa ctctagaata tctagcaaat
5161 agttaaagaa gcaatttatt attagggcat actcgggctg tttccaaata taaactctat
5221 tgcaatatct tatttcatct ttctaataca tgtacagtgc acactagagg atagagctgc
5281 atcacttaaa ttcatgactt aaaaaataat acagtttata tacaacttgt tttttatttg
5341 attaagaagt gaagtttatg ccacccaatg tatagccaaa ttgtacgtgc ttaaaaaaca
5401 gtgccgagag tatgttcagt tcgcagtaag tagatttatt ggaataaata ttctatggta
5461 cattctcaga aattggcttc caactaaaat acgtttgacc cattttgaat aaggaaattg
5521 aaaagaaaaa tttaaaagga gaaaaaaatg catgtttata aactttttaa ataaaaccag
5581 accttgtaag tggacattaa aaaaaaaaaa aaaaaaa
//