LOCUS BC146671 4663 bp mRNA linear HUM 12-JUL-2007
DEFINITION Homo sapiens mannosidase, endo-alpha, mRNA (cDNA clone MGC:164944
IMAGE:40148201), complete cds.
ACCESSION BC146671
VERSION BC146671.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4663)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4663)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (16-JUN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Novartis Institute for Biomedical Research
cDNA Library Preparation: Novartis Institute for Biomedical
Research
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 306 Row: i Column: 19.
FEATURES Location/Qualifiers
source 1..4663
/db_xref="H-InvDB:HIT000435535"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:164944 IMAGE:40148201"
/tissue_type="Donated clones,Novartis FGA collection"
/clone_lib="NIH_MGC_417"
/lab_host="DH5a"
/note="Vector: pCMV-SPORT6"
gene 1..4663
/gene="MANEA"
/gene_synonym="hEndo"
/db_xref="GeneID:79694"
/db_xref="HGNC:HGNC:21072"
CDS 116..1504
/gene="MANEA"
/gene_synonym="hEndo"
/codon_start=1
/product="mannosidase, endo-alpha"
/protein_id="AAI46672.1"
/db_xref="GeneID:79694"
/db_xref="HGNC:HGNC:21072"
/translation="MAKFRRRTCIILALFILFIFSLMMGLKMLRPNTATFGAPFGLDL
LPELHQRTIHLGKNFDFQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNY
LHVFYYSWYGNPQFDGKYIHWNHPVLEHWDPRIAKNYPQGRHNPPDDIGSSFYPELGS
YSSRDPSVIETHMRQMRSASIGVLALSWYPPDVNDENGEPTDNLVPTILDKAHKYNLK
VTFHIEPYSNRDDQNMYKNVKYIIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPE
KWANLLTTSGSRSIRNSPYDGLFIALLVEEKHKYDILQSGFDGIYTYFATNGFTYGSS
HQNWASLKLFCDKYNLIFIPSVGPGYIDTSIRPWNTQNTRNRINGKYYEIGLSAALQT
RPSLISITSFNEWHEGTQIEKAVPKRTSNTVYLDYRPHKPGLYLELTRKWSEKYSKER
ATYALDRQLPVS"
BASE COUNT 1665 a 746 c 686 g 1566 t
ORIGIN
1 cggcagtcag ctctatgttc gcggtcttaa cctctcctct ggccgagtcc ttgcaagaag
61 tgaattaccc gaccctgcaa aacacttact attttggagt ttaaagtatg tcatcatggc
121 aaagtttcgg agaaggactt gcatcatttt ggcacttttt attctattta ttttctctct
181 gatgatgggt ttaaaaatgc tgagaccaaa tacagctact tttggagctc cttttggact
241 tgaccttctt ccagaacttc atcaacgaac tattcatttg gggaaaaatt ttgatttcca
301 aaagagtgac agaatcaaca gtgaaacaaa taccaagaat ttaaaaagtg ttgaaatcac
361 tatgaaacct tccaaagcct ctgaacttaa cttggatgaa ctaccacctc tgaacaatta
421 tctacatgta ttttattaca gttggtatgg aaatccacaa tttgatggta aatatataca
481 ttggaatcat ccagtgttag agcattggga ccctagaata gccaagaatt atccacaagg
541 gagacacaac cctccagatg acattggctc cagcttttat cctgaattgg gaagttacag
601 ttctcgggat ccttctgtca tagaaactca catgagacaa atgcgctcag cttcaattgg
661 tgtactagcc ctctcttggt acccacctga tgtaaatgat gaaaatggag aacctactga
721 taacttggta cccactattt tggataaagc tcataaatat aacctaaagg ttacttttca
781 catagaacca tatagcaatc gagatgatca aaacatgtac aaaaatgtca agtatattat
841 agacaaatat ggaaatcatc cggcctttta caggtacaag acgaagactg gcaatgctct
901 tcctatgttt tatgtctatg attcctatat taccaagcct gaaaaatggg ccaatctgtt
961 aaccacctca gggtctcgga gtattcgcaa ttctccttat gatggactgt ttattgccct
1021 tctggtagaa gaaaaacata agtatgatat tcttcaaagt ggttttgatg gaatttacac
1081 atattttgcc acaaatggct ttacttatgg ctcatcacat cagaattggg ctagcctaaa
1141 attattttgt gataaataca acttaatatt tatcccaagt gtgggcccag gatacataga
1201 taccagcatc cgtccatgga acacgcaaaa cactcggaac cgaatcaatg ggaagtatta
1261 tgaaattggt ctgagtgccg cacttcagac acgccccagc ttaatttcta tcacctcttt
1321 taatgagtgg catgaaggaa ctcagattga aaaagctgtt cccaaaagaa ccagtaatac
1381 agtgtaccta gattaccgtc ctcataaacc aggtctttac ctagaactga ctcgcaagtg
1441 gtctgaaaaa tacagtaagg aaagagcaac ttatgcatta gatcgccagc tgcctgtttc
1501 ttaatgcatt gattaaagtt taatagttat caaaatcacc taatttttaa aaatagcttt
1561 cgttttgagt tctggaaaga aaactgtcaa aatcagtata tactattagt tatatttaaa
1621 aatatttttt ttaattcttt acagataata ttatacttgt tacccttcac aataccacat
1681 gagaaaatat ctgagacaaa atgtatacaa atatattcct tatggcataa tttattgcat
1741 ttctgactga aatcaaaatt ctgatttgat ggcaattgaa ttttcatttt acaatagata
1801 aatgcttgtg ttacctaaag cacttagcac acagttaaat tatatttaca tcctagaccc
1861 aaataaatag gattgtgtgt atatttggga tatctattga agaaaaaaag aaaacccctt
1921 aaagataatg tacatgcttc atgtcatgtc tttaaaataa tttaatcaac tttattgtct
1981 tagtatttag actctggata actctacaat aatgaggaaa ttcttaagaa taacaaaatc
2041 actgtacctt cctctcaatt ttgctgtgaa cctgaaatgg ctttaaatta atactcttat
2101 tttttattta atttaattac ataaattaaa ccttaccatg accaaattgt gttaggaagg
2161 cctgctatct acagcacagt gtgtcatttg cagatttgtg gttacctata ccacgctagg
2221 tgttttgaca tgtttagtgt ttctgcttta cagtgctgaa ttccatattt tagaagctat
2281 gaaagtcctt ttatgaaaaa gttactgatt gcttctcagt tattaggaaa acagttgttt
2341 cacaattatt atgtagatat gatgcccaaa tatcattttt agtatatctt gtcgatcttt
2401 aagttgttac tattgtgtta ttcatgtctt taaatcagat accaaatatt ttttaggaaa
2461 gaaaaatgtt attactgtca ttaggttgtc ttttaatact ttaagttatt ttgacgaaaa
2521 gtaatagaga aaatttactt agcattttag attctagaga catggaaatg aaaattattt
2581 tatgtctaga gtaggtcctg aagtttggct ttacattaag tttagcactg tatcagaatg
2641 aagaaactaa tattttacat aaaaactaat actttcaatt ttttatatag taatatcccc
2701 attttgtaaa tgttagactt ttatcatacc tgtaagttaa aatacttgtt atcaataact
2761 tgtcatattg tgacaaattg atcacttgtg tacgaaaaat aaatctcctt aaaaactaaa
2821 taaaatgcac tgtattctta cagttaatgt ttataactat agtaaaaaat taatatatat
2881 cctattacat aaatgttatt tcttaggtgt tccattaaga agagcaatag aataatgcta
2941 aaaaataatg cctataaatc ttcagagtat aaagacatcc attcagaaac aaaaattagc
3001 actaaatttt ttataaaata gaccagatga caaaatttat tttattttta aacagtggtt
3061 ttgacacaaa ttatgttatt gaaaagcatt attaatgttt aatttattta aaattttgga
3121 atttgccatt tctcagacaa tgatcaggcc ttaggaaatt aatacagtag tagtaatcat
3181 tttctagggg aaaataaaag aataaatcac tatactgata ttttgatata agcaagcact
3241 tacatggtaa tcactatata gatccaacct gtggattttc ttcttatgtc catttaacta
3301 gaatatatta ttttaggtat aatttacaaa tgtcacacct aataatcttt tataatatac
3361 catatttcat taaagttttg ttagagaagt atctaccaca gaggagtttt tgtcattgtg
3421 tacgttgtgt atttgaaccc accatgacag aaagtaaatt ttaggaaata gttatgagat
3481 taagggaaaa tctataaaaa caaggttagc atattctcaa cacagatacc accactttct
3541 ttttcccatt atagacatgg tgaatccaca cagcatactt catctctgag ctttgttgtg
3601 attcctcaac acattaccct aaccagccag cagtaacaga tttcagagta agataaagca
3661 gattctgtct tcattgcaaa aagttattct caatggaaga atggcatctg atctcataat
3721 tactagttta tattaatata gtttttttct ccctttttaa taaaataatt acagtcatcc
3781 ctcagtgtct gtgggggatt ggttccagtt acccctatag ataccaaaat ctgcagatgc
3841 tcaagtccct gatataaact ggcatagtag ttgcatataa tctatgcaca tcctcctgta
3901 tacattaagt catctctaga ttatttataa cacttaatac aatgtaaatg ctatgtagtt
3961 gttataccat actggttagt gaataattac atgaaaaaaa agagtctgta catcttcaga
4021 gtttcagtcg gcaatttctt ggccatggat gtagaaccta cagataaggt gagccaactg
4081 cattaggaaa taactctaat aattctgtta attcttagga aaactttcaa aatcttcctc
4141 aggtatttat tacaactgcc tttaccattt tagttgtaac acagtttaaa ttgttatgat
4201 aacaagtaaa taagagcaaa gaatttattt cttaattcaa aactatacgt ttgaattcaa
4261 tatggtataa cttaaagtgg tataatacat acaatgcatg aatcataatg gattctttta
4321 taagttatta atttttatgg tttaatcagt ctaattgttt tgactgttat agaaaccaaa
4381 tattttactg tttcttttaa ggactaatat tgtcaaaaac tgctgttatt aacttcactt
4441 gagttgttta acttccttct gttttaagat tgtaattaaa aattactatt ttgttatatg
4501 gaatggttaa tttttaccta ataaaaacat agatgaaata cattgtaaaa aaaaaaaaaa
4561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//