LOCUS BC144033 2532 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens guanylate cyclase 1, soluble, alpha 2, mRNA (cDNA
clone MGC:177570 IMAGE:9052553), complete cds.
ACCESSION BC144033
VERSION BC144033.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2532)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2532)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JUN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 17 Row: G Column: 1.
FEATURES Location/Qualifiers
source 1..2532
/db_xref="H-InvDB:HIT000502469"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:177570 IMAGE:9052553"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_362"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: AGCATAAG"
gene 1..2532
/gene="GUCY1A2"
/gene_synonym="GC-SA2"
/db_xref="GeneID:2977"
/db_xref="HGNC:HGNC:4684"
/db_xref="MIM:601244"
CDS 97..2358
/gene="GUCY1A2"
/gene_synonym="GC-SA2"
/codon_start=1
/product="GUCY1A2 protein"
/protein_id="AAI44034.1"
/db_xref="GeneID:2977"
/db_xref="HGNC:HGNC:4684"
/db_xref="MIM:601244"
/translation="MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPG
PLEPSPAAAAAAAAPAPTPAASAAAAAATAGARRVQRRRRVNLDSLGESISRLTAPSP
QTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYADHSNKEEIEDVSGILQCTAN
ILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHIRTSFGKQA
TLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKL
CSDVSNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFD
PSMSVLQLGEGLRKQLRCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKP
EASGSENKDKSKHVTEGHLTQLSVAGFNSLEVMEVKGQMIHVPESNSILFLGSPCVDK
LDELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEK
KKTVDLLYSIFPGDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISM
LNELYTRFDHQCGFLDIYKVETIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEV
LTPDGRPIQMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPT
TYQLLKREESFTFIPRSREELPDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVS
YNIGTMFLRETSL"
BASE COUNT 661 a 626 c 633 g 612 t
ORIGIN
1 cttaacgttg tcgcttgccg gtcccgccac cgccgcctcc gccgccgctc gcgtcctcgc
61 cgccaccgcc tcggccgctg cagctccgcc ggcagcatgt ctcgaaggaa gatttcgtcc
121 gagtccttca gctccctggg ctccgactac ctggagacca gcccggagga ggagggggag
181 tgccccctgt ctaggctctg ctggaatggc agccggagcc cgcccgggcc gctggagccc
241 agcccggccg cagctgccgc tgccgccgct ccggccccga ccccggctgc ttctgccgcc
301 gccgccgctg ccactgccgg ggccaggagg gtgcagcgcc ggaggcgggt caacctggac
361 tcgctgggcg agagcatcag ccgcctgacg gcgccctcgc ctcagacgat acagcagact
421 ctcaagagga cactgcagta ttatgaacat caagttattg gttacaggga tgcagaaaag
481 aatttccaca atatctctaa cagatgctcc tatgcagacc actccaacaa agaagaaatt
541 gaagatgtct caggaattct tcagtgtact gctaatatac tcggtttgaa gtttgaggaa
601 attcaaaaaa gatttggtga agagttcttt aatatatgct ttcatgagaa tgagagagtc
661 cttcgagctg taggtggcac tttgcaggac ttttttaacg gctttgatgc tttgttggaa
721 cacattagaa cttcttttgg aaaacaggcc actctggagt caccatcttt cctatgcaaa
781 gagctccctg aaggtactct catgctccac tacttccacc ctcaccatat tgtggggttt
841 gcaatgctgg ggatgattaa ggctgcagga aagaagatct atcggctgga tgtggaagtg
901 gaacaggttg caaatgagaa gctatgctct gatgtttcaa acccaggcaa ttgtagctgt
961 cttactttcc ttatcaaaga atgtgaaaat actaatatca tgaagaacct tccacaggga
1021 acctcccaag ttcctgcgga cctcagaatt agcatcaaca ccttctgtag agccttccct
1081 ttccacttga tgtttgatcc cagcatgtca gtccttcagt tgggggaagg tctaaggaag
1141 cagcttcgat gtgacactca caaagtgctc aagtttgagg actgcttcga gattgtatct
1201 ccaaaggtta atgccacctt tgaaagggtc ctgctgcgac tgtctacccc gtttgtgatt
1261 agaaccaagc ctgaggcttc tggctctgaa aataaagaca agagcaagca tgtcaccgag
1321 gggcatctga cgcagttatc agtggcagga ttcaacagtc ttgaggtgat ggaagtcaaa
1381 ggacaaatga tccatgttcc agaatcaaat tccattttat ttttgggctc tccatgtgtg
1441 gacaagttgg atgaactcat gggccgaggg ctacatctct cagacatccc tatccatgat
1501 gccacccgag atgtcatttt ggttggtgag caggcaaagg cccaagatgg gttgaagaaa
1561 aggatggata aattaaaggc aactttagaa agaactcacc aggccctgga agaagagaaa
1621 aagaagacag tggatcttct atattctatt ttccctggtg atgtagccca gcaattatgg
1681 caagggcagc aagtacaggc cagaaagttt gatgatgtca ccatgctctt ttcagacatt
1741 gttggcttca cagccatatg tgcccagtgt actcccatgc aagtaatcag catgctgaat
1801 gaactgtaca ccagatttga ccaccagtgt ggatttttgg atatttataa ggtggaaaca
1861 ataggtgatg cctactgtgt tgcagcaggg ctccacagaa aaagcctctg ccatgctaaa
1921 cccattgctc tgatggcctt gaagatgatg gaactttcag aagaggtgct gacacctgat
1981 ggaagaccga ttcagatgag gataggaatt cactcaggct ccgtgctggc tggagttgtt
2041 ggggtgcgaa tgccacgtta ttgcctgttt ggaaataatg tcacactggc aagcaaattc
2101 gagtcgggaa gtcaccctcg gcgcatcaat gtcagcccaa ccacttacca attattaaaa
2161 cgagaagaaa gtttcacatt cattccgcgg tctcgtgaag agcttccaga caactttcca
2221 aaggaaattc ctgggatctg ctatttcctg gaggtaagga ctggtccaaa gccaccaaag
2281 ccttctcttt cttcgtcgag aataaaaaag gtttcctaca acatcggcac catgttcctc
2341 cgggagacaa gcctctgaga cctgctacag atcaaagact cctccaaaaa gcacaagccc
2401 agaacatggg tcaccaatgg ggggtggaaa gagattgtgt ctctttcatt gctttgttga
2461 gaacaagcag caaaatttct gtattatgtc aggcaataat cctactaaaa ggtggaggtg
2521 accgctgtca at
//