LOCUS BC143868 3601 bp mRNA linear HUM 08-JAN-2009
DEFINITION Homo sapiens phospholipase C, beta 4, mRNA (cDNA clone MGC:177401
IMAGE:9052384), complete cds.
ACCESSION BC143868
VERSION BC143868.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3601)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3601)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JUN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 16 Row: F Column: 21.
FEATURES Location/Qualifiers
source 1..3601
/db_xref="H-InvDB:HIT000502309"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:177401 IMAGE:9052384"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_311"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: AGATGGGC"
gene 1..3601
/gene="PLCB4"
/gene_synonym="PI-PLC"
/db_xref="GeneID:5332"
/db_xref="HGNC:HGNC:9059"
/db_xref="MIM:600810"
CDS 34..3561
/gene="PLCB4"
/gene_synonym="PI-PLC"
/codon_start=1
/product="phospholipase C, beta 4"
/protein_id="AAI43869.1"
/db_xref="GeneID:5332"
/db_xref="HGNC:HGNC:9059"
/db_xref="MIM:600810"
/translation="MAKPYEFNWQKEVPSFLQEGTVFDRYEEESFVFEPNCLFKVDEF
GFFLTWRSEGKEGQVLECSLINSIRSGAIPKDPKILAALEAVGKSENDLEGRIVCVCS
GTDLVNISFTYMVAENPEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNT
NGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPTAFSYEKFYELTQKICP
RTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYE
PDEDLKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTG
RQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAI
KETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRALPS
PNDLKRKILIKNKRLKPEVEKKQLEALRSMMEAGESASPANILEDDNEEEIESADQEE
EAHPEFKFGNELSADDLGHKEAVANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLS
TMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYP
KGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRR
PDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRT
RMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRH
ISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMG
IETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALASGVEAKKGIELI
PQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEK
STHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSEMINT
HSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENS
KAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEF
LEKQNEQAKEMQQMVKLEAEMDRRPATVV"
BASE COUNT 1232 a 725 c 804 g 840 t
ORIGIN
1 tcagtccaga gctgcccagt cttgaatata atcatggcca aaccttatga atttaactgg
61 cagaaggaag ttccctcctt tttgcaagaa ggaacagttt ttgacagata cgaggaggaa
121 tcctttgtgt ttgaacccaa ctgcctcttc aaagtggatg agtttggctt ctttctgaca
181 tggagaagtg aaggcaagga aggacaggtg ctagaatgct ccctcatcaa cagtattcgg
241 tcgggagcca taccaaagga tcccaaaatc ttggctgctc ttgaagctgt tggaaaatca
301 gaaaatgatc tggaagggcg gatagtttgt gtctgcagtg gcacagatct agtgaacatt
361 agttttacct acatggtggc tgaaaatcca gaagtaacta agcaatgggt agaaggcctg
421 agatcaatca tacacaactt cagggccaac aacgtcagtc caatgacatg cctcaagaaa
481 cactggatga aattggcatt tatgaccaac acaaatggta aaattccagt taggagtatt
541 actagaacat ttgcatcggg aaaaacagaa aaggtgatct ttcaagcact caaggagtta
601 ggtcttccca gtggaaagaa tgatgaaatt gagcccacag cattttctta tgaaaagttc
661 tatgaactga cacaaaagat ttgtcctcgg acagatatag aagatctttt caaaaaaatc
721 aatggagaca aaactgatta tttaacggta gaccaattag tgagctttct aaatgaacat
781 caacgagatc ctcgattgaa tgaaatttta tttccatttt atgatgccaa aagggcaatg
841 cagatcattg agatgtatga acctgatgaa gatttgaaga aaaaaggcct tatatcaagt
901 gatgggtttt gcagatatct gatgtcagat gaaaacgccc cagtcttcct agatcgttta
961 gaactttacc aagaaatgga ccatcctctg gctcactact tcatcagttc ttcccataac
1021 acttatctca ctggcagaca gttcggcggg aagtcttcgg tagaaatgta cagacaggtt
1081 ctcctggctg gttgcagatg tgttgaactt gactgctggg atggaaaagg tgaagaccaa
1141 gaaccaataa taactcatgg aaaagcaatg tgtacagata tcctttttaa ggatgtaatt
1201 caagccatca aggaaactgc atttgtcaca tcagaatatc ctgtaattct ctcctttgaa
1261 aatcactgca gcaaatatca acagtacaag atgtccaaat attgcgaaga tctatttggg
1321 gatctcctgt tgaaacaagc acttgaatca catccacttg aaccaggcag ggctttgcca
1381 tcccccaatg acctcaaaag aaaaatactc ataaaaaaca agcggctgaa acctgaagtt
1441 gaaaaaaaac agctggaagc tttgagaagc atgatggaag ctggagaatc tgcctcccca
1501 gcaaacatct tagaggacga taatgaagag gagatcgaaa gtgctgacca agaggaggaa
1561 gctcaccccg aattcaaatt tggaaatgaa ctttctgctg atgacttggg tcacaaggaa
1621 gctgttgcaa atagcgtcaa gaagggcctg gtcactgtag aagatgagca ggcgtggatg
1681 gcatcttata aatatgtagg tgctaccact aatatccatc catatttgtc cacaatgatc
1741 aactacgccc agcctgtaaa gtttcaaggt ttccatgtgg cagaagaacg caatattcat
1801 tataacatgt cttcttttaa tgaatcagtc ggtcttggct acttgaagac acatgcaatt
1861 gaatttgtca attataacaa acggcaaatg agtcgcattt accccaaggg aggccgagtc
1921 gattccagta attacatgcc tcagattttc tggaacgctg gctgccagat ggtttcactg
1981 aactatcaaa ccccagattt agcgatgcaa ttgaatcagg gaaaatttga gtataatgga
2041 tcgtgcgggt accttctcaa accagatttc atgaggcggc ctgatcgaac atttgacccc
2101 ttctctgaaa ctcctgttga tggtgttatt gcagccactt gctcagtgca ggttatatca
2161 ggtcaattct tatcagataa gaaaattggc acctacgtag aggtggatat gtatgggttg
2221 cccactgaca ccatacgtaa ggaattccga actcgcatgg ttatgaataa tggactcaat
2281 ccagtttaca atgaagagtc atttgtattt cggaaggtga tcctgccgga cctggctgtc
2341 ttgagaatag ctgtgtatga tgataacaac aagctgattg gccagaggat cctcccgctt
2401 gatggcctcc aagccggata tcgacacatt tcccttcgaa atgagggaaa taaaccatta
2461 tcactaccaa caattttctg caatattgtt cttaaaacat atgtgcctga tggatttgga
2521 gatatcgtgg atgctttatc agatccaaag aaatttctct caattacaga aaagagagca
2581 gaccaaatga gagctatggg cattgaaact agtgacatag ccgacgtgcc cagtgacact
2641 tccaaaaatg acaagaaagg aaaggccaac accgccaaag caaatgtgac ccctcagagt
2701 agctctgagc tcagaccaac caccacggct gccctggcct ctggtgtgga agccaagaaa
2761 ggtattgaac ttatccctca agtaaggata gaagacttaa agcagatgaa ggcttacttg
2821 aagcatttaa agaaacagca gaaggagcta aattctttaa agaagaaaca tgcaaaggaa
2881 cacagtacca tgcagaagtt acactgcacg caagttgaca aaattgtggc acagtatgac
2941 aaagagaagt cgactcatga gaaaatccta gagaaggcaa tgaagaagaa ggggggaagt
3001 aattgtctcg aaatgaaaaa agaaacagaa atcaaaattc agacgctgac atcagatcac
3061 aaatctaagg tcaaagagat tgtagcacag cacacaaagg aatggtcaga aatgatcaat
3121 acccacagtg ctgaggagca agaaatccga gacctgcacc tcagccagca gtgtgagctg
3181 ctgaaaaagc tactcatcaa tgcccacgag cagcaaaccc agcagctgaa actgtcccat
3241 gacagggaaa gcaaggaaat gcgagcacac caggctaaga tttctatgga aaatagcaaa
3301 gccatcagcc aagataaatc tatcaagaat aaagcagaac gggaaaggcg agtcagggag
3361 ttaaacagca gcaacactaa aaagtttctg gaagaaagaa agagacttgc catgaagcag
3421 tccaaagaaa tggatcagtt gaaaaaagtc cagcttgaac atctagaatt cctagagaaa
3481 cagaatgagc aggcgaagga gatgcagcag atggtgaaat tggaagccga gatggaccgc
3541 agaccagcaa cagtagtatg aaactccaaa atgcaaactg aagcagcaaa cccacaaagc
3601 a
//