LOCUS BC143744 3952 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens AF4/FMR2 family, member 2, mRNA (cDNA clone MGC:177275
IMAGE:9052258), complete cds.
ACCESSION BC143744
VERSION BC143744.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3952)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3952)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JUN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 16 Row: G Column: 10.
FEATURES Location/Qualifiers
source 1..3952
/db_xref="H-InvDB:HIT000502187"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:177275 IMAGE:9052258"
/tissue_type="Cerebellum, Lung, PCR rescued clones"
/clone_lib="NIH_MGC_347"
/lab_host="DH10B"
/note="Vector: pCR-XL-TOPO; Clone identification sequence
tag: GGCTAACA"
gene 1..3952
/gene="AFF2"
/gene_synonym="FRAXE"
/gene_synonym="MRX2"
/gene_synonym="OX19"
/db_xref="GeneID:2334"
/db_xref="HGNC:HGNC:3776"
/db_xref="MIM:309548"
CDS 52..3882
/gene="AFF2"
/gene_synonym="FRAXE"
/gene_synonym="MRX2"
/gene_synonym="OX19"
/codon_start=1
/product="AFF2 protein"
/protein_id="AAI43745.1"
/db_xref="GeneID:2334"
/db_xref="HGNC:HGNC:3776"
/db_xref="MIM:309548"
/translation="MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLF
SSGFDLFGEPYKVAEYTNKGDALANRVQNTLGNYDEMKNLLTNHSNQNHLVGIPKNSV
PQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVILNSTLIHSNRKSKPEWS
RDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNPSAKE
DSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYC
KTSMGQQKPTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSK
TKLPKFTILQTSEVSLPSDPSCVEEILRESQHLTPGFTLQKWNDPTTRASTKMLEDDL
KLSSDEDDLEPVKTLTTQCTATELYQAVEKAKPRNNPVNPPLATPQPPPAVQASGGSG
SSSESESSSESDSDTESSTTDSESNEAPRVATPEPEPPSTNKWQLDKWLNKVTSQNKS
FICGQNETPMETISLPPPIIQPMEVQMKVKTNASQVPAEPKERPLLSLIREKARPRPT
QKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTSTDEFTWPKPNITSSTPKEKE
SVELHDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREFIETDSST
SDSNTDQEETLQIKVLPPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEE
PTFSPIPVMQTEILSPLRDHENLKNLWVKIDLDLLSRVPGHSSLHAAPAKPDHKETAT
KPKRQTAVTAVEKPAPKGKRKHKPIEVAEKIPEKKQRLEEATTICLLPPCISPAPPHK
PPNTRENNSSRRANRRKEEKLFPPPLSPLPEDPPRRRNVSGNNGPFGQDKNIAMTGQI
TSTKPKRTEGKFCATFKGISVNEGDTPKKASSATITVTNTAIATATVTATAIVTTTVT
ATATATATTTTTTTTISTITSTITTGLMDSSHLEMMSWAALPLLSSSSTNVRRPKLTF
DDSVHNADYYMQEAKKLKHKADALFEKFGKAVNYADAALSFTECGNAMERDPLEAKSP
YTMYSETVELLRYAMRLKNFASPLASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKY
SRSLMEYFKQNASKVAQIPSPWVSNGKNTPSPVSLNNVSPINAMGNCNNGPVTIPQRI
HHMAASHVNITSNVLRGYEHWDMADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVRYV
RQGLCWLRIDAHLL"
BASE COUNT 1212 a 1096 c 830 g 814 t
ORIGIN
1 acccgctgcg atcagggaca ggcgcccgcc cgccgccgcc gcctggccgc tatggatcta
61 ttcgactttt tcagagactg ggacttggag cagcagtgtc actatgaaca agaccgtagt
121 gcacttaaaa aaagggaatg ggagcggagg aatcaagaag tccagcaaga agacgatctc
181 ttttcttcag gctttgatct ttttggggag ccatacaagg tagctgaata tacaaacaaa
241 ggtgatgcac ttgccaaccg agtccagaac acgcttggaa actatgatga aatgaagaat
301 ttgctaacta accattctaa tcagaatcac ctagtgggaa ttccaaagaa ttctgtgccc
361 cagaatccca acaacaaaaa tgaaccaagc ttttttccag aacaaaagaa cagaataatt
421 ccacctcacc aggataatac ccatccttca gcaccaatgc ctccaccttc tgttgtgata
481 ctgaattcaa ctctaataca cagcaacaga aaatcaaaac ctgagtggtc acgtgatagt
541 cataacccta gcactgtact ggcaagccag gccagtggtc agccaaacaa gatgcagact
601 ttgacacagg accagtctca agccaaactg gaagacttct ttgtctaccc agctgaacag
661 ccccagattg gagaagttga agagtcaaac ccatctgcaa aggaagacag taaccctaat
721 tctagtggag aagatgcttt caaagaaatc tttcaatcca attcaccgga agaatctgaa
781 ttcgccgtgc aagcgcctgg gtctccccta gtggcttcct ctttattagc tcctagcagt
841 ggcctttcag ttcaaaactt cccaccaggg ctttactgca aaacaagcat ggggcagcaa
901 aagccaactg catacgtcag acccatggat ggccaggacc aggcaccgga catctcacca
961 acactgaaac cttcaattga atttgagaac agctttggga atctgtcatt tggaacactc
1021 ttggatggaa aacccagtgc agccagttca aagactaaac tgccaaagtt caccatcctc
1081 caaacaagtg aagtaagcct tcccagtgat ccaagctgtg ttgaagaaat cttgcgggaa
1141 tcgcagcatc tgaccccagg attcacctta caaaagtgga atgacccaac caccagagct
1201 tctacaaaga tgcttgagga tgacctgaag ctgagcagtg atgaagatga ccttgagcct
1261 gtgaagacct tgaccactca gtgcactgcc actgagctct accaggctgt tgaaaaggca
1321 aaacctagga ataatcctgt gaacccaccc ttggccactc cccagccccc acctgcagtg
1381 caagccagcg ggggttctgg cagctccagc gaatcggaga gcagctctga gtcggattca
1441 gacactgaaa gtagcaccac tgacagcgaa tctaatgagg cacctcgtgt ggcaactcca
1501 gagcctgagc caccctcaac caacaagtgg caactggata aatggcttaa caaagtgaca
1561 tcccagaaca agtcttttat ttgtggccaa aatgaaacac ccatggagac tatttctctg
1621 cctcctccaa tcatccaacc aatggaagtc cagatgaaag tgaagacgaa tgccagtcag
1681 gtcccagctg aacccaaaga aaggcctctc ctcagtctca ttagggagaa agcccgtcca
1741 cggcccactc agaaaattcc agaaacaaag gctttgaagc ataagttgtc aacaactagt
1801 gagacagtgt ctcaaaggac aattgggaaa aaacagccca aaaaagttga gaagaacacc
1861 agcactgacg agtttacctg gcccaaacca aatattacca gcagcactcc caaagaaaaa
1921 gaaagtgtgg agcttcatga cccaccaaga ggccgcaaca aagccactgc ccacaaacca
1981 gcccctagga aagaaccaag acctaacatc cctttggctc ccgagaagaa gaagtacaga
2041 gggcctggca agattgtgcc aaagtctcgg gaattcattg aaacagattc atctacatct
2101 gactccaaca cagatcagga agagaccctg caaatcaaag tcctgcctcc gtgcattatt
2161 tctggaggta atactgccaa atccaaggaa atctgtggtg ccagcctgac cctcagcacc
2221 ttaatgagta gcagtggcag caacaacaac ttatccatca gtaatgaaga gccaacattt
2281 tcacctattc ctgtcatgca aactgaaatc ctgtcccctc tgcgagatca tgagaacctg
2341 aaaaacctct gggtgaagat tgaccttgac ttactctcta gagtacctgg ccacagctca
2401 ctccatgcag cacctgccaa gccagaccac aaggagactg ccacaaaacc caagcgtcag
2461 acagctgtca cagctgtgga gaaaccagcc cctaagggca aacgtaagca caagccaata
2521 gaagttgcag agaagatccc tgagaagaag cagcgcctgg aggaggccac aactatctgc
2581 ttgctccctc cttgcatctc accagcccca ccccacaagc ctcccaacac tagagaaaat
2641 aattcatcca ggagagcaaa tagaagaaag gaagaaaaac tatttcctcc tccactttcc
2701 ccactgccag aggaccctcc acgccgcaga aatgtcagtg gcaataatgg tccctttggt
2761 caagacaaaa acatcgccat gactggacaa atcacatcta ccaaacctaa gagaactgaa
2821 ggcaaattct gtgctacttt caaagggata tcggtaaatg agggagacac tccaaaaaag
2881 gcatcctctg ccaccatcac tgtcaccaat actgctattg ccactgctac tgtcactgct
2941 actgccattg tcaccaccac tgtcacagct actgccaccg ccacggccac caccacaact
3001 actaccacta ccatttccac catcacctct accatcacta ctggcctcat ggatagcagt
3061 cacctggaga tgatgtcctg ggcggctctg ccccttctat ccagcagcag cactaatgtc
3121 cggagaccca agctcacttt tgatgactcg gttcacaatg ctgattatta catgcaagaa
3181 gctaagaagc tgaagcacaa agctgatgca ctgttcgaga aatttggcaa agctgtgaat
3241 tatgctgatg ccgccctctc cttcactgaa tgtggcaatg ccatggaacg cgaccctctg
3301 gaagcaaagt ccccatacac catgtactct gagactgtgg agctcctcag gtatgcaatg
3361 aggctgaaga actttgcaag tcccttggct tcggatgggg acaaaaagct agcagtacta
3421 tgctaccgat gtttatcact cctctatttg agaatgttta agctgaagaa ggaccatgct
3481 atgaagtact ccagatcact gatggaatat tttaagcaaa atgcttcaaa agtcgcacag
3541 ataccctctc catgggtaag caatggaaag aacactccat ccccagtgtc tctcaacaac
3601 gtctccccca tcaacgcaat ggggaactgt aacaatggcc cagtcaccat tccccagcgc
3661 attcaccaca tggctgccag ccacgtcaac atcactagca atgtgttacg gggctatgaa
3721 cactgggata tggccgacaa actgacaaga gaaaacaaag aattctttgg tgatctggac
3781 acgctgatgg ggcctctgac ccagcacagc agcatgacca atcttgtccg ctacgttcgc
3841 caaggactgt gttggctgcg catcgatgcc cacttgttgt agtgggtgtt ctcagatctc
3901 tagcatcacg acccatcact ctacctctac cagcgcactg atggtcactg gt
//