LOCUS BC140891 6638 bp mRNA linear HUM 12-MAY-2008
DEFINITION Homo sapiens mediator complex subunit 13, mRNA (cDNA clone
MGC:176568 IMAGE:9021759), complete cds.
ACCESSION BC140891
VERSION BC140891.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6638)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 6638)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-MAY-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 673 Row: p Column: 13.
FEATURES Location/Qualifiers
source 1..6638
/db_xref="H-InvDB:HIT000501631"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:176568 IMAGE:9021759"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_313"
/note="Vector: pCR-XL-TOPO with reversed insert; Clone
identification sequence tag: TGCTGACC"
gene 1..6638
/gene="MED13"
/gene_synonym="ARC250"
/gene_synonym="DRIP250"
/gene_synonym="HSPC221"
/gene_synonym="TRAP240"
/db_xref="GeneID:9969"
/db_xref="HGNC:HGNC:22474"
/db_xref="MIM:603808"
CDS 52..6576
/gene="MED13"
/gene_synonym="ARC250"
/gene_synonym="DRIP250"
/gene_synonym="HSPC221"
/gene_synonym="TRAP240"
/codon_start=1
/product="mediator complex subunit 13"
/protein_id="AAI40892.1"
/db_xref="GeneID:9969"
/db_xref="HGNC:HGNC:22474"
/db_xref="MIM:603808"
/translation="MSASFVPNGASLEDCHCNLFCLADLTGIKWKKYVWQGPTSAPIL
FPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPSFADLIHHDLSEE
EDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHL
SCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILCPFGLNGTLT
GQAFKMSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRM
IYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEV
QTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKR
KYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQ
QILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRF
SNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTP
DPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKK
DVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCL
SAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSH
EEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGS
KKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKE
YGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGC
SMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEY
GTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASA
QGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDC
NFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDEL
DIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFG
AADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLH
PWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRG
SYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPEN
EALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFS
QAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMT
NTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSS
NKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHP
DVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLR
CFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPL
PTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAG
QKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQK
LWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCG
ISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCT
HILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILP
ASPTGSPVHSPGSHYPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKA
GPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRF
VLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML"
BASE COUNT 1941 a 1428 c 1396 g 1873 t
ORIGIN
1 gtaatggcgg atggtgggtt gtggcgccgg cggcggctgc tgtgagggac gatgagtgcc
61 tccttcgtgc cgaacggggc cagcctggaa gattgtcact gtaacctctt ctgcctggct
121 gacttgacag gaattaagtg gaaaaaatat gtatggcaag gcccaacttc tgcccctatt
181 ctgtttcctg tgacagaaga agaccccatt ttgagcagtt ttagtcgctg ccttaaggca
241 gatgtacttg gtgtttggcg gcgagatcaa agacctggaa gaagagaatt gtggatattt
301 tggtggggtg aagaccccag ttttgctgac cttattcacc atgacttatc agaagaagaa
361 gatggagtgt gggagaatgg actttcctat gaatgccgta ctctgctttt caaagcagtt
421 cacaatctat tggaacggtg tttaatgaac aggaattttg tacgtattgg caagtggttt
481 gtaaagcctt atgaaaaaga tgaaaaacct ataaataaaa gtgaacactt gtcctgctcc
541 ttcacctttt tcttgcatgg agacagcaat gtttgtacca gtgtggaaat taaccaacat
601 caacctgtat accttctcag tgaagagcat atcacccttg ctcaacagtc taatagccca
661 tttcaagtta tcttatgccc atttggacta aatggcactc tcacaggaca ggcattcaag
721 atgtctgatt cagctacaaa aaaattaatt ggtgaatgga aacagttcta tcctatctca
781 tgttgcttga aggagatgtc tgaagaaaaa caggaagata tggattggga agatgattct
841 ttagctgcag tagaagttct tgttgctggt gtccgaatga tctacccagc atgctttgtt
901 ctagtccctc agtcagacat tcctactcct agccctgtgg gatccactca ctgttcatct
961 tcttgcttgg gtgtccacca agtgcctgct tccacaagag atcctgctat gtcttcggtt
1021 acgcttacac cacctacgtc tcctgaggaa gtccaaacag ttgatcctca gtctgtccag
1081 aagtgggtca aattttcttc agtatctgat ggcttcaact ccgatagtac tagccaccat
1141 ggtgggaaaa tacccagaaa attagcaaat catgtggtgg atagagtttg gcaagaatgc
1201 aatatgaaca gagcacagaa caagaggaag tattctgctt catcaggtgg tctatgcgaa
1261 gaagcgacag ctgctaaagt ggcatcctgg gattttgttg aagccacaca aagaacaaat
1321 tgcagttgtt tgaggcacaa aaatctcaag tcaagaaatg ctggacaaca aggacaggca
1381 ccatctttag gtcagcaaca acaaatactt cctaagcaca agaccaatga gaagcaagaa
1441 aagagtgaaa agccacagaa acgccccttg actccttttc accatcgtgt gtctgttagt
1501 gatgatgttg gcatggacgc agattcagcc agccaaagac ttgtgatctc tgctccagac
1561 agtcaagtga gattttcaaa tatccgaact aatgatgtag caaagactcc tcagatgcat
1621 ggcaccgaaa tggcaaattc acctcaacca cccccactta gtcctcaccc ttgtgatgtg
1681 gttgatgaag gagtgactaa aacaccttca actcctcaga gtcaacattt ttatcaaatg
1741 ccaacaccag atcccttggt tccttctaaa ccaatggaag ataggataga cagtttgtcc
1801 cagtctttcc cacctcaata tcaggaagct gtagaaccta cagtatatgt tggtacagca
1861 gtaaacttgg aagaagatga agccaatata gcctggaagt attacaagtt cccaaagaaa
1921 aaagatgtag agtttttacc acctcaacta ccaagtgata aattcaagga tgatccagtt
1981 ggaccttttg gacaggaaag tgtaacatca gttacagagt taatggtgca atgtaagaaa
2041 cctttaaaag tttctgatga attagtgcag caatatcaaa ttaaaaacca gtgtctttca
2101 gcaatagcat ctgatgcaga acaagaacct aaaattgatc catatgcatt tgttgaagga
2161 gatgaggaat tcctttttcc tgataaaaaa gatagacaaa atagtgagag agaagctgga
2221 aaaaaacaca aggtagaaga tgggacatct agtgtaacag tgttatcaca tgaagaagat
2281 gctatgtcat tatttagtcc ctctatcaag caagatgctc cacgccctac tagtcatgcc
2341 cgtcctccat caacaagttt gatttatgac tcagacctgg ctgtctctta tactgacctt
2401 gataatctct tcaattctga tgaagatgaa ctaacacctg gatctaaaaa atcagcaaat
2461 ggatcagatg ataaagccag ctgcaaggaa tcaaagacag gaaatctgga cccattatct
2521 tgcataagca ctgcagatct tcataaaatg tatcctacac caccatcatt ggaacaacat
2581 attatgggat tttccccaat gaatatgaat aataaagaat atggtagtat ggatacaaca
2641 cctggaggaa ctgttctaga aggaaatagt tctagtatag gagcgcagtt caaaattgag
2701 gttgatgagg gattctgtag ccccaaacct tctgaaatta aagatttttc ttatgtctat
2761 aagcctgaaa attgtcaaat tctagtggga tgttccatgt ttgcacctct aaaaactcta
2821 ccaagccaat atctgccccc tatcaaattg ccagaagagt gtatttaccg tcagagttgg
2881 actgttggaa aattggaatt gctttcttca gggccttcaa tgccattcat caaagagggt
2941 gatggaagta atatggatca agaatatggc actgcttata cacctcaaac tcatacttct
3001 tttgggatgc ctcctagcag tgcacctcct agtaacagcg gagcaggaat tcttccttct
3061 ccatccaccc ctcggtttcc aactccaagg actccaagga ctcctcggac tcctcgtgga
3121 gctggtggac ctgctagtgc tcaaggttca gtcaaatatg aaaattcaga cttgtattca
3181 ccagcttcta ccccatctac atgcagaccc cttaattctg ttgaacctgc aactgtccct
3241 tccatccctg aagcacacag tctttatgta aacctcatcc tttcagaatc agttatgaat
3301 ttgtttaaag actgtaactt tgatagttgt tgcatctgtg tttgcaacat gaacatcaag
3361 ggtgccgatg ttggagttta cattccagat ccaacgcagg aagcacaata taggtgtacc
3421 tgtggcttca gtgctgtcat gaacagaaaa tttggaaaca attcaggatt atttcttgaa
3481 gatgaactag atatcatagg acgcaataca gactgtggca aagaagcaga aaaacgtttt
3541 gaagctctca gggctacctc tgctgaacat gttaatggag gactaaagga atctgaaaaa
3601 ttatctgatg atttgatatt attgctacaa gatcagtgca ctaatttatt ttcacccttt
3661 ggagcagcag accaagatcc ttttcctaaa agtggtgtaa ttagcaattg ggtacgtgtt
3721 gaagagcgtg actgttgcaa tgactgctac cttgcattag aacatgggcg tcagttcatg
3781 gataacatgt caggaggaaa agttgatgaa gcacttgtga aaagttcatg cttacacccc
3841 tggtccaaaa gaaacgatgt gagtatgcag tgctcacagg atatacttcg aatgctcctc
3901 tctcttcagc cagttcttca ggatgccatt cagaaaaaaa gaacagtaag accttggggt
3961 gttcagggtc ctctcacttg gcaacaattt cataaaatgg ctggccgagg ctcttatgga
4021 actgatgaat ccccagaacc actgccaatc cccacatttt tgttgggtta tgattatgat
4081 tatctggtgc tttctccatt tgctcttcct tattgggaga gacttatgct ggaaccctat
4141 ggatctcaaa gagatatagc ctatgttgta ctgtgtccag aaaatgaagc cttgttaaat
4201 ggagcaaaaa gcttttttag agatcttact gcaatatatg agtcctgtcg attaggtcaa
4261 catagacctg tttctcgact gttaacagat gggatcatga gagttggatc tactgcatca
4321 aagaaactat cagaaaagtt ggtagcagaa tggttttctc aggcagctga tggtaacaat
4381 gaagcatttt ctaaactcaa gctttatgca caagtctgca gatatgacct aggtccttat
4441 cttgcttccc tgccattgga cagctctcta ctttcccagc caaatttagt tgcccctaca
4501 agtcagtctt tgattactcc acctcagatg acaaatactg gaaatgctaa tactccatct
4561 gccaccttag catctgcagc gagcagcact atgacagtga cttcaggtgt tgccatatct
4621 acttcagttg ccacagctaa ttcaactttg accacagctt caacttcatc ttcatcatcc
4681 tccaacttga atagtggagt atcatcaaat aaactacctt cgtttccacc ctttggcagt
4741 atgaacagta atgctgcagg atccatgtct acacaagcaa atacagttca gagtggtcag
4801 ctaggagggc aacagacatc agctctacag acagctggga tttctggaga atcatcttca
4861 cttcccactc agccgcatcc tgatgtgtct gaaagcacga tggatcggga taaagtggga
4921 atccccacag atggtgattc acatgcagtc acgtatccac ctgcaattgt tgtttatata
4981 attgatcctt ttacatacga aaatacagac gagagcacta actcttctag tgtgtggaca
5041 ttggggctac ttcgatgctt tctagaaatg gtccagactc ttcctcctca tatcaagagt
5101 actgtttctg tacagattat tccttgtcag tacctgttgc aacctgtgaa gcatgaagat
5161 agagaaatct atccccagca tttaaaatcc ctggcttttt cggcctttac ccagtgtcgg
5221 aggccacttc caacatcaac caatgtgaaa acattgactg gctttggtcc aggtttagcc
5281 atggaaactg cccttagaag tcctgataga ccagagtgta ttcgacttta tgcacctcct
5341 tttattctgg ctccagtgaa ggacaaacag acagagctag gagaaacatt tggagaagct
5401 ggacagaaat ataatgttct ttttgtggga tactgtttat cacatgatca aaggtggatt
5461 cttgcatctt gcacagatct atatggagaa cttttagaaa cttgtatcat taacatcgat
5521 gttccaaata gggctcgtcg gaaaaaaagt tctgctagaa aatttggtct acagaaactt
5581 tgggagtggt gcttaggact tgtacaaatg agttcattgc catggagagt tgtaattggt
5641 cgtctaggaa ggattggtca tggagaattg aaagattgga gctgtttgct gagtcgtcga
5701 aacttgcagt ctctaagtaa aaggctcaaa gacatgtgta gaatgtgtgg tatatctgct
5761 gcagactccc ctagcattct cagtgcttgc ttggtggcaa tggagccgca aggctctttt
5821 gttattatgc cagattctgt gtcaactggt tctgtatttg gaagaagcac gactctaaat
5881 atgcagacat ctcagctaaa taccccacag gatacatcat gtactcatat acttgtgttt
5941 cctacttctg cttctgtgca agtagcttca gctacttata ccactgaaaa tttggattta
6001 gctttcaatc ccaacaatga tggagcagat ggaatgggta tctttgattt gttagacaca
6061 ggagatgatc ttgaccctga tatcattaat atccttcctg cttctccaac tggttctcct
6121 gtacattctc caggatctca ttacccccat ggaggtgatg cgggcaaggg tcagagtact
6181 gatcggctac tatcaacaga acctcatgag gaagtaccta atattcttca gcaaccattg
6241 gcccttggtt actttgtatc aactgccaaa gcaggtccat tacctgactg gttctggtca
6301 gcatgtcctc aagcacaata tcagtgtccc ctttttctta aggcctcttt gcacctccac
6361 gtgccttcag tgcaatctga cgagctgctt cacagtaaac actcccaccc acttgactca
6421 aatcagactt cagatgtcct caggtttgtt ttggaacagt acaatgcact ctcctggcta
6481 acctgtgacc ctgcaaccca ggacagacgc tcatgtctcc caattcattt tgtggtgctg
6541 aatcagttat ataactttat tatgaatatg ctgtgatctt catttgatgg aactgtgcaa
6601 gaaaagaaca aggaaaaatg gatgtttcgc tgcaggat
//