LOCUS BC140768 4444 bp mRNA linear HUM 11-FEB-2009
DEFINITION Homo sapiens vacuolar protein sorting 8 homolog (S. cerevisiae),
mRNA (cDNA clone MGC:176445 IMAGE:9021636), complete cds.
ACCESSION BC140768
VERSION BC140768.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4444)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4444)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-MAY-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 13 Row: A Column: 18
single stranded.
FEATURES Location/Qualifiers
source 1..4444
/db_xref="H-InvDB:HIT000501522"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:176445 IMAGE:9021636"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_313"
/note="Vector: pCR-XL-TOPO with reversed insert; Clone
identification sequence tag: CAATCTCA"
gene 1..4444
/gene="VPS8"
/db_xref="GeneID:23355"
/db_xref="HGNC:HGNC:29122"
CDS 108..4394
/gene="VPS8"
/codon_start=1
/product="vacuolar protein sorting 8 homolog (S.
cerevisiae)"
/protein_id="AAI40769.1"
/db_xref="GeneID:23355"
/db_xref="HGNC:HGNC:29122"
/translation="MENEPDHENVEQSLCAKTSEEELNKSFNLEASLSKFSYIDMDKE
LEFKNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFILEDPTLLNIDTIDSHSYDT
SSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAI
AVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGF
AKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRV
MGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPS
LKVWMTFPYGRMDPSSVPLLAWHFVAVQNYVNPMLAFCRGDVVHFLLVKRDESGAIHV
TKQKHLHLYYDLINFTWINSRTVVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSS
HFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLL
KQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILFHYADRALKKCPD
QGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQMYDKLSENSVAKGVFLECLEPYIL
SDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENRLYDAM
IYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPL
GDIPEDLVPLVKNQVFEFLIRLHSAEASPEEEIYPYIRTLLHFDTREFLNVLALTFED
FKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLF
DQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYE
REHQYDKIIDCYLRDPLREEEVFNYIHNILSIPGHSAEEKQSVWQKAMDHIEELVSLK
PCKAAELVATHFSGHIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQELLQISPCITE
QFIELLCQFNPTQVIETLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDIHGAFLI
MLERLQSKLQEVTHQGENTKEDPSLKDVEDTMVETIALCQRNSHNLNQQQREALWFPL
LEAMMAPQKLSSSAIPHLHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKL
GEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICL
QQYKRRQEMADEIIVFSCGHLYHSFCLQNKECTVEFEGQTRWTCYKCSSSNKVGKLSE
NSSEIKKGRITPSQVKMSPSYHQSKGDPTAKKGTSEPVLDPQQIQAFDQLCRLYRGSS
RLALLTELSQNRSSESYRPFSGSQSAPAFNSIFQNENFQLQLIPPPVTED"
BASE COUNT 1343 a 891 c 979 g 1231 t
ORIGIN
1 ggaatggcag caagctcaga gataatcatt caggtcttcg tgagctaagg ccaaacaaac
61 aaaaaacact tgctttgatg gactgaatac tgttattaga agtaaatatg gaaaatgaac
121 cagaccatga aaatgtggaa cagagcctct gtgccaagac gagcgaagaa gagctgaata
181 agtctttcaa tctagaagct tcactttcaa aattctctta catagatatg gacaaggaac
241 tggagttcaa aaatgatctg attgatgaca aggagtttga tattcctcaa gttgatactc
301 ctccaacact ggaaagcata ctaaatgaga ctgatgatga agatgagtct tttattcttg
361 aggatcctac attgttaaac attgatacta ttgattctca ctcctatgat acttcatctg
421 tggcaagctc agatagtggt gacaggacca acttaaaaag gaagaagaaa ttacctgatt
481 ctttttcact tcatggatca gttatgcgcc attcactttt gaagggaatt tctgcccaga
541 tagtgtctgc agctgacaaa gtagatgctg gcttgcctac agcaattgca gtatccagtc
601 tgatagcagt gggtacatct catggattgg ctttaatatt tggaaaagat cagaatcaag
661 ctttgcgact ctgtctgggt agcactagtg ttggaggtca gtatggcgct atctctgccc
721 tcagtatcaa caatgattgc tcaagacttc tttgtggctt tgctaaagga cagatcacca
781 tgtgggattt ggccagtgga aaacttctaa gatcaataac agatgctcat cctccaggaa
841 cagcaatatt gcatatcaag tttacagatg atccaactct tgcaatttgc aacgacagcg
901 gaggctctgt ttttgaattg acatttaaga gagtgatggg agtgagaacc tgtgaatcta
961 gatgtctgtt cagtggttcc aagggtgaag tctgctgtat tgagcctctg cattctaagc
1021 ctgagttgaa agatcatccc atcacacagt tttcattatt ggccatggca tccttgacaa
1081 aaatactggt cattggattg aaaccatcct tgaaagtatg gatgactttt ccctatggcc
1141 ggatggatcc ttccagtgtg ccactgctgg cctggcactt tgtagcagta caaaattacg
1201 tgaatcccat gcttgccttc tgcagaggag atgttgttca ttttctattg gtaaagagag
1261 atgaatctgg agcaatacat gttactaagc aaaagcatct tcacctatac tatgacctca
1321 tcaactttac ctggataaat tcacgcacag ttgtgctctt agacagcgta gagaagttgc
1381 atgtgattga tcggcaaaca caagaggaat tggagacagt ggagatctca gaagttcagc
1441 tggtctacaa tagcagccat ttcaaatcac tagccactgg aggaaatgtt agccaggcac
1501 tggctttggt tggagagaag gcttgttatc aatccatcag tagctatggt ggtcagatct
1561 tttatttggg gacaaaatct gtttatgtga tgatgctgag gagctggaga gagagagtgg
1621 atcatctcct gaaacaagat tgtcttacag aagcgttggc tcttgcgtgg tctttccatg
1681 aaggaaaagc aaaagcagta gtgggattat caggggatgc cagtaagcga aaggctattg
1741 ttgcagaccg gatggtagaa atcctattcc attatgcaga tcgagctctg aaaaagtgcc
1801 cagaccaagg aaaaatccaa gtgatggagc agcattttca ggatatggtg cctgtcatag
1861 ttgattactg ccttctgctg cagcgaaagg atcttttatt tagtcagatg tatgataaat
1921 taagtgagaa ttcagtggcc aaaggagtat ttttggagtg ccttgagcca tatattttaa
1981 gtgataaatt ggtgggaatc acaccccaag taatgaaaga cttgattgtt catttccaag
2041 ataaaaaatt aatggaaaat gtggaagcgc tcattgtaca tatggatatc accagcctag
2101 atattcagca ggtagttctc atgtgttggg aaaatcgttt atatgatgct atgatctatg
2161 tctacaacag aggcatgaat gaatttatta gtccaatgga gaaacttttc agagtcattg
2221 ctcctcctct gaatgcagga aaaacactaa cagatgaaca agttgttatg ggcaataagc
2281 tccttgtata tattagctgt tgtctagcag gtcgtgccta tccccttggt gacatccctg
2341 aagatctggt tcccttggtt aaaaaccagg tttttgaatt tctaattcgc ctgcattcag
2401 cagaggcttc tcctgaggaa gaaatctatc cttacattcg gactttgcta cattttgaca
2461 caagagaatt tctcaatgta ttggcactga cttttgaaga ttttaaaaat gacaagcaag
2521 ctgtggaata tcaacagcga attgtggata ttttgttgaa agttatggtg gagaattcag
2581 actttacccc ctcacaagta ggatgtctct ttaccttcct tgctcggcag cttgcaaagc
2641 ctgacaacac cttgtttgta aacagaacac tttttgatca ggtccttgaa ttcctttgta
2701 gtcctgacga tgactcccga cactctgaaa gacagcaggt ccttttagaa ttgctgcagg
2761 ctggaggcat agttcaattt gaagagagtc gactcatccg gatggcagaa aaagctgagt
2821 tctatcaaat ttgtgaattt atgtatgaaa gagaacacca atatgacaaa attattgatt
2881 gctacttacg tgaccctctg cgagaggaag aagtctttaa ttacattcac aatatcttat
2941 ccattcccgg acacagtgca gaggagaagc agtctgtatg gcagaaagca atggatcata
3001 ttgaggaact cgtgtccctg aagccttgta aagctgcgga gctggttgcc acccactttt
3061 ctggacatat tgaaacggtc attaaaaaac ttcagaacca ggttttgctt ttcaaatttt
3121 tgaggagtct tcttgaccca agggaaggta ttcatgtaaa tcaagaatta ctgcaaatat
3181 ctccttgtat cacagagcag ttcattgagc tgttgtgtca gttcaaccca acccaagtta
3241 tagagactct gcaagtcctt gagtgctacc gtctggaaga aactattcag attactcaga
3301 agtatcaact tcatgaagtc accgcttatc tattggaaaa gaaaggagat attcatggtg
3361 ccttcctaat aatgttagag agactacaaa gcaaacttca agaggtaaca catcaaggtg
3421 aaaataccaa agaggatccc tcattgaagg atgttgaaga tactatggtg gagaccattg
3481 ctctttgcca gagaaattca cataatttga accagcagca acgtgaggcc ctttggtttc
3541 cgttattgga ggcaatgatg gcccctcaga agctgtccag ttcagccatt cctcatctac
3601 actctgaagc tctgaagtct ttgaccatgc aagttttaaa tagcatggca gcatttattg
3661 cccttccatc aatcttgcaa agaatcttac aggatccagt ttatggaaaa ggaaaacttg
3721 gagaaatcca gggacttatc ttgggaatgt tagatacctt taactatgaa caaaccctgc
3781 tggaaacaac aaccagcctt ctaaaccaag atctccattg gtcattgtgt aacctgagag
3841 cttcggtcac cagaggactg aatcccaaac aagattactg ctctatatgt ttgcagcagt
3901 acaagagacg ccaagaaatg gctgatgaaa taattgtctt tagctgtggc catttgtatc
3961 actcattctg cctacaaaac aaagaatgca ctgtggaatt tgagggccaa acaagatgga
4021 catgctacaa atgcagttca agtaacaaag taggaaaact cagtgaaaat tcatctgaaa
4081 ttaaaaaggg aaggataacc ccatcacagg taaaaatgtc tccatcgtat catcagtcca
4141 aaggggatcc cactgctaaa aagggaacct cagaacctgt tctggatcca cagcaaatcc
4201 aagcatttga tcagctttgc cgtctctacc gaggaagctc caggctggct ctcctcacgg
4261 aactctccca gaatcgcagc agcgagagct ataggccatt cagtggctcg cagagtgctc
4321 ctgctttcaa cagcatcttc cagaatgaga acttccagct gcagctcatt cctccacctg
4381 tgactgagga ttgatgactc catggagcct ggcccaggag aaccagagat gatcccgagg
4441 cagc
//