LOCUS BC139729 1583 bp mRNA linear HUM 05-APR-2007
DEFINITION Homo sapiens mitogen-activated protein kinase kinase 1, mRNA (cDNA
clone MGC:133320 IMAGE:40053655), complete cds.
ACCESSION BC139729
VERSION BC139729.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1583)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1583)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (04-APR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCG Plate: 16 Row: e Column: 10.
FEATURES Location/Qualifiers
source 1..1583
/db_xref="H-InvDB:HIT000391163"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:133320 IMAGE:40053655"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_282"
/note="Vector: pCR-Blunt II-TOPO; Clone identification
sequence tag: CCCTTTTT sequenced from the reverse primer"
gene 1..1583
/gene="MAP2K1"
/gene_synonym="MAPKK1"
/gene_synonym="MEK1"
/gene_synonym="MKK1"
/db_xref="GeneID:5604"
/db_xref="HGNC:HGNC:6840"
/db_xref="MIM:176872"
CDS 60..1241
/gene="MAP2K1"
/gene_synonym="MAPKK1"
/gene_synonym="MEK1"
/gene_synonym="MKK1"
/codon_start=1
/product="mitogen-activated protein kinase kinase 1"
/protein_id="AAI39730.1"
/db_xref="GeneID:5604"
/db_xref="HGNC:HGNC:6840"
/db_xref="MIM:176872"
/translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDE
QQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLE
IKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFG
CQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEF
QDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAG
V"
BASE COUNT 388 a 382 c 432 g 381 t
ORIGIN
1 gaggaagcga gaggtgctgc cctccccccg gagttggaag cgcgttaccc gggtccaaaa
61 tgcccaagaa gaagccgacg cccatccagc tgaacccggc ccccgacggc tctgcagtta
121 acgggaccag ctctgcggag accaacttgg aggccttgca gaagaagctg gaggagctag
181 agcttgatga gcagcagcga aagcgccttg aggcctttct tacccagaag cagaaggtgg
241 gagaactgaa ggatgacgac tttgagaaga tcagtgagct gggggctggc aatggcggtg
301 tggtgttcaa ggtctcccac aagccttctg gcctggtcat ggccagaaag ctaattcatc
361 tggagatcaa acccgcaatc cggaaccaga tcataaggga gctgcaggtt ctgcatgagt
421 gcaactctcc gtacatcgtg ggcttctatg gtgcgttcta cagcgatggc gagatcagta
481 tctgcatgga gcacatggat ggaggttctc tggatcaagt cctgaagaaa gctggaagaa
541 ttcctgaaca aattttagga aaagttagca ttgctgtaat aaaaggcctg acatatctga
601 gggagaagca caagatcatg cacagagatg tcaagccctc caacatccta gtcaactccc
661 gtggggagat caagctctgt gactttgggg tcagcgggca gctcatcgac tccatggcca
721 actccttcgt gggcacaagg tcctacatgt cgccagaaag actccagggg actcattact
781 ctgtgcagtc agacatctgg agcatgggac tgtctctggt agagatggcg gttgggaggt
841 atcccatccc tcctccagat gccaaggagc tggagctgat gtttgggtgc caggtggaag
901 gagatgcggc tgagacccca cccaggccaa ggacccccgg gaggcccctt agctcatacg
961 gaatggacag ccgacctccc atggcaattt ttgagttgtt ggattacata gtcaacgagc
1021 ctcctccaaa actgcccagt ggagtgttca gtctggaatt tcaagatttt gtgaataaat
1081 gcttaataaa aaaccccgca gagagagcag atttgaagca actcatggtt catgctttta
1141 tcaagagatc tgatgctgag gaagtggatt ttgcaggttg gctctgctcc accatcggcc
1201 ttaaccagcc cagcacacca acccatgctg ctggcgtcta agtgtttggg aagcaacaaa
1261 gagcgagtcc cctgcccggt ggtttgccat gtcgcttttg ggcctccttc ccatgcctgt
1321 ctctgttcag atgtgcattt cacctgtgac aaaggatgaa gaacacagca tgtgccaaga
1381 ttctactctt gtcattttta atattactgt ctttattctt attactatta ttgttcccct
1441 aagtggattg gctttgtgct tggggctatt tgtgtgtatg ctgatgatca aaacctgtgc
1501 caggctgaat tacagtgaaa ttttggtgaa tgtgggtagt cattcttaaa attgcactgc
1561 tgttcctgct ccatgactgg ctg
//