LOCUS BC137459 1279 bp mRNA linear HUM 12-MAY-2008
DEFINITION Homo sapiens mitogen-activated protein kinase kinase 1, mRNA (cDNA
clone MGC:169082 IMAGE:9021459), complete cds.
ACCESSION BC137459
VERSION BC137459.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1279)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1279)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 673 Row: d Column: 1.
FEATURES Location/Qualifiers
source 1..1279
/db_xref="H-InvDB:HIT000501357"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:169082 IMAGE:9021459"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_311"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: TGCCTTGA"
gene 1..1279
/gene="MAP2K1"
/gene_synonym="MAPKK1"
/gene_synonym="MEK1"
/gene_synonym="MKK1"
/db_xref="GeneID:5604"
/db_xref="HGNC:HGNC:6840"
/db_xref="MIM:176872"
CDS 64..1245
/gene="MAP2K1"
/gene_synonym="MAPKK1"
/gene_synonym="MEK1"
/gene_synonym="MKK1"
/codon_start=1
/product="mitogen-activated protein kinase kinase 1"
/protein_id="AAI37460.1"
/db_xref="GeneID:5604"
/db_xref="HGNC:HGNC:6840"
/db_xref="MIM:176872"
/translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDE
QQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLE
IKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFG
CQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEF
QDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAG
V"
BASE COUNT 327 a 319 c 366 g 267 t
ORIGIN
1 gagggaggaa gcgagaggtg ctgccctccc cccggagttg gaagcgcgtt acccgggtcc
61 aaaatgccca agaagaagcc gacgcccatc cagctgaacc cggcccccga cggctctgca
121 gttaacggga ccagctctgc ggagaccaac ttggaggcct tgcagaagaa gctggaggag
181 ctagagcttg atgagcagca gcgaaagcgc cttgaggcct ttcttaccca gaagcagaag
241 gtgggagaac tgaaggatga cgactttgag aagatcagtg agctgggggc tggcaatggc
301 ggtgtggtgt tcaaggtctc ccacaagcct tctggcctgg tcatggccag aaagctaatt
361 catctggaga tcaaacccgc aatccggaac cagatcataa gggagctgca ggttctgcat
421 gagtgcaact ctccgtacat cgtgggcttc tatggtgcgt tctacagcga tggcgagatc
481 agtatctgca tggagcacat ggatggaggt tctctggatc aagtcctgaa gaaagctgga
541 agaattcctg aacaaatttt aggaaaagtt agcattgctg taataaaagg cctgacatat
601 ctgagggaga agcacaagat catgcacaga gatgtcaagc cctccaacat cctagtcaac
661 tcccgtgggg agatcaagct ctgtgacttt ggggtcagcg ggcagctcat cgactccatg
721 gccaactcct tcgtgggcac aaggtcctac atgtcgccag aaagactcca ggggactcat
781 tactctgtgc agtcagacat ctggagcatg ggactgtctc tggtagagat ggcggttggg
841 aggtatccca tccctcctcc agatgccaag gagctggagc tgatgtttgg gtgccaggtg
901 gaaggagatg cggctgagac cccacccagg ccaaggaccc ccgggaggcc ccttagctca
961 tacggaatgg acagccgacc tcccatggca atttttgagt tgttggatta catagtcaac
1021 gagcctcctc caaaactgcc cagtggagtg ttcagtctgg aatttcaaga ttttgtgaat
1081 aaatgcttaa taaaaaaccc cgcagagaga gcagatttga agcaactcat ggttcatgct
1141 tttatcaaga gatctgatgc tgaggaagtg gattttgcag gttggctctg ctccaccatc
1201 ggccttaacc agcccagcac accaacccat gctgctggcg tctaagtgtt tgggaagcaa
1261 caaagagcga gtcccctgc
//