LOCUS BC137438 3248 bp mRNA linear HUM 12-MAY-2008
DEFINITION Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial, mRNA (cDNA
clone MGC:169059 IMAGE:9021436), complete cds.
ACCESSION BC137438
VERSION BC137438.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3248)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3248)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 673 Row: c Column: 2.
FEATURES Location/Qualifiers
source 1..3248
/db_xref="H-InvDB:HIT000501340"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:169059 IMAGE:9021436"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_311"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: GGGCGGCG"
gene 1..3248
/gene="IARS2"
/db_xref="GeneID:55699"
/db_xref="HGNC:HGNC:29685"
CDS 75..3113
/gene="IARS2"
/codon_start=1
/product="isoleucyl-tRNA synthetase 2, mitochondrial"
/protein_id="AAI37439.1"
/db_xref="GeneID:55699"
/db_xref="HGNC:HGNC:29685"
/translation="MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRS
VSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRE
RKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPI
EIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGK
YEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLL
KPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAA
DKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVH
TAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTA
KNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALN
GMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWW
TLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEG
KDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGG
QDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADF
NPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFY
FSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPK
SVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFE
IIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREE
SSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK"
BASE COUNT 914 a 649 c 806 g 879 t
ORIGIN
1 cttggtttcc tggggtcctg ccccttcaag ctggggcggg agcggaggac cccgctctca
61 ggggttgccg gaccatgcgt tgggggctgc gccctcgcgg gccgggcgcg gccgccctgg
121 ccactgcccg aagtttgtgg gggacgcccc gccttccctg cagcccggga tggcaagggg
181 cgacgaagag gcttctggtg cggtcggtct ccggggccag taaccaccag ccgaactcga
241 atagtggcag ataccgggac acggtgctgc tgccgcagac gagcttcccc atgaagctgc
301 tgggccgcca gcagccggac acggagctgg agatccagca gaaatgtgga ttttcagaac
361 tttattcatg gcaaagagaa agaaaagtaa agacagaatt ttgccttcat gatggacctc
421 cttatgcaaa cggtgaccct catgttggac atgctttaaa taagattttg aaagacatag
481 ccaatcgatt ccatatgatg aatggctcca aaatacattt tgtgcccggc tgggattgtc
541 atgggttgcc cattgaaata aaagtattat cagaacttgg tagagaagct cagaatcttt
601 cagctatgga aattagaaag aaagctagat catttgctaa agcagccatt gagaaacaga
661 aatcagcatt tattcgttgg ggaataatgg cagattggaa taattgctac tatacatttg
721 atgggaagta tgaagccaaa cagttgagaa ctttttacca aatgtatgat aagggcttgg
781 tttatcgatc ttacaaacct gtgttttggt ctccgtcatc taggactgca ttggctgaag
841 cagaacttga atataatcct gagcatgtca gtcgttcaat atatgtaaaa tttcctctct
901 taaagccttc tccaaaattg gcatctctta tagatggttc atctcctgtt agtattttgg
961 tctggaccac acaaccttgg acgattccag ccaatgaagc tgtttgctat atgcctgaat
1021 caaagtatgc tgttgtgaaa tgttctaagt ctggagacct ctacgtactg gcggcagata
1081 aagtagcatc tgttgcttct actttggaaa caacatttga gactatttca acactttcag
1141 gtgtagattt ggaaaatggt acttgcagtc atccattaat tcctgataaa gcctctcctc
1201 ttttacctgc aaatcatgtg accatggcaa aaggaacggg attggttcac acagccccag
1261 ctcatggtat ggaagactac ggtgtagcgt ctcagcacaa cctgcccatg gattgtctag
1321 tggacgaaga tggagttttc acagatgttg caggtcctga acttcaaaac aaggctgtcc
1381 ttgaagaggg aactgatgtg gttataaaga tgcttcagac tgcaaagaat ttgttgaaag
1441 aggagaaatt ggtgcatagc tatccgtatg actggaggac caagaaacct gtggttattc
1501 gtgccagcaa gcagtggttt ataaacatca cggatattaa gactgcagcc aaggaattgt
1561 taaaaaaggt gaaatttatt cctggatcag cactgaatgg catggttgaa atgatggaca
1621 ggcggccata ttggtgtata tcaaggcaaa gagtttgggg tgttccaatt cctgtgtttc
1681 atcataagac caaggatgaa tacttgatca acagccaaac cactgagcat attgttaaac
1741 tagtggaaca acacggcagt gatatctggt ggactcttcc ccctgaacaa cttcttccaa
1801 aagaagtctt atctgaggtt ggtggccctg atgccttgga atatgtgcca ggtcaggata
1861 ttttggacat ctggtttgat agcggaactt catggtctta tgttcttcca ggtcctgacc
1921 aaagagcaga tttgtacttg gaaggaaaag accagctcgg gggttggttt cagtcatcct
1981 tattaacaag tgtggcagca aggaagagag caccttataa gacagtgatt gttcatggat
2041 ttacccttgg agaaaaggga gaaaagatgt ccaagtctct tgggaatgtc attcatcctg
2101 atgttgtcgt taatggagga caagatcaaa gcaaagagcc tccgtatggt gctgatgtcc
2161 ttcgctggtg ggtagctgat tccaatgtct tcaccgaagt tgcaattggc ccatccgtgc
2221 tcaatgctgc cagagatgat attagcaagc ttaggaatac acttcgcttt cttttgggaa
2281 atgtggctga tttcaaccca gaaacagatt ccatccctgt aaacgatatg tatgtcatag
2341 accagtacat gctacactta ctgcaggatt tggcaaacaa gattaccgaa ttatacaaac
2401 aatatgattt tggaaaagtt gttcggctgt tacggacgtt ttataccaga gagctctcta
2461 acttttattt cagtataatc aaagataggc tctattgtga aaaggaaaat gaccccaaac
2521 gacgctcttg tcagactgca ttagttgaaa ttttggatgt aatagttcgt tcttttgctc
2581 ccattcttcc tcacctggct gaagaggtgt tccagcacat accttatatt aaagagccca
2641 agagtgtttt ccgtactggg tggattagta ctagttctat ctggaaaaag cccgggttgg
2701 aagaagctgt ggagagtgcg tgtgcaatgc gagactcatt tcttggaagc atccctggca
2761 aaaatgcagc tgagtacaag gttatcactg tgatagaacc tggactgctt tttgagataa
2821 tagagatgct gcagtctgaa gagacttcca gcacctctca gttgaatgaa ttaatgatgg
2881 cttctgagtc aactttactg gctcaggaac cacgagagat gactgcagat gtaatcgagc
2941 ttaaagggaa attcctcatc aacttagaag gtggtgatat tcgtgaagag tcttcctata
3001 aagtaattgt catgccgact acgaaagaaa aatgcccccg ttgttggaag tatacagcgg
3061 agtcttcaga tacactgtgt cctcgatgtg cagaagttgt cagtggaaaa tagtattaac
3121 agctcactcg agcaagaacc ctcctgacag tactggctag aagtttggat ggattattta
3181 caatatagga aagaaagcca agatttaggt aatgagtgga tgagtaaatg gtggaggatg
3241 ggagtcaa
//