LOCUS BC137226 6933 bp mRNA linear HUM 12-MAY-2008
DEFINITION Homo sapiens AF4/FMR2 family, member 4, mRNA (cDNA clone MGC:168846
IMAGE:9021223), complete cds.
ACCESSION BC137226
VERSION BC137226.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6933)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 6933)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 672 Row: j Column: 5.
FEATURES Location/Qualifiers
source 1..6933
/db_xref="H-InvDB:HIT000501180"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:168846 IMAGE:9021223"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_313"
/note="Vector: pCR-XL-TOPO with reversed insert; Clone
identification sequence tag: AAGCGCAC"
gene 1..6933
/gene="AFF4"
/gene_synonym="AF5Q31"
/gene_synonym="MCEF"
/db_xref="GeneID:27125"
/db_xref="HGNC:HGNC:17869"
/db_xref="MIM:604417"
CDS 307..3798
/gene="AFF4"
/gene_synonym="AF5Q31"
/gene_synonym="MCEF"
/codon_start=1
/product="AF4/FMR2 family, member 4"
/protein_id="AAI37227.1"
/db_xref="GeneID:27125"
/db_xref="HGNC:HGNC:17869"
/db_xref="MIM:604417"
/translation="MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSK
EDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGS
HQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSG
SSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDA
NWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYS
SQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAI
HTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDS
DGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSD
SEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGRE
QGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQ
PKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPT
AEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVE
EEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGE
KKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNR
ESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSK
NSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYS
ADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSE
TVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTE
HLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQK
IHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVR
YTRQGLHWLRQDAKLIS"
BASE COUNT 2074 a 1527 c 1465 g 1867 t
ORIGIN
1 caggtcttcg ttaacccgcc cccgccgcct cagtcagctg tcccagcccg ggcccgaccg
61 tcatggagcg gtgcggggcc tacgcctgtt ccgtgtgagg cgcatcgctg ccgccgccgc
121 cgacgccgcc ttcgtttgtc gaggcctccg gaacccccgc cgcctcccgg agcccctcgc
181 ccttggcagc ctgtcaccgc cccctgggcg ggctcgaatg cgcgtccggt gaaggtgcag
241 gcccggcgcc gccactgccg cagccaggag atggttcggg cctagcggag ccgggactgg
301 agcaacatga accgtgaaga ccggaatgtg ctgcgtatga aagaacggga aaggcggaat
361 caggaaattc agcagggcga agacgccttc ccacctagct ctcctctctt tgcagagcca
421 tacaaagtta ctagcaaaga agataagtta tcaagtcgta ttcagagtat gcttggaaac
481 tacgatgaaa tgaaggattt cataggagac agatctatac caaagcttgt tgcaattccc
541 aagcctacag taccaccatc agcagatgaa aaatctaacc caaatttctt tgaacagaga
601 catggaggct ctcatcagag tagcaaatgg actccagtag gacccgcacc cagcacttct
661 cagtctcaga aacggtcctc aggcttacag agtggacata gtagccagcg gaccagcgca
721 ggtagcagta gtggcactaa cagtagtggt cagaggcacg accgtgagtc atataacaat
781 agtgggagca gtagccggaa aaaaggccag catggatcag aacactccaa atcacgttct
841 tccagccctg gaaaacccca ggctgtttct tcattaaact ctagtcattc caggtctcat
901 gggaatgatc accatagcaa ggaacatcaa cgctccaaat cacctcggga ccctgatgca
961 aactgggatt ctccttcccg tgtacctttt tcaagtgggc agcactcaac tcaatctttc
1021 ccaccctcat tgatgtcaaa gtccaattca atgttacaga aacccactgc ctatgtgcgg
1081 cccatggacg gacaggagtc catggaacca aagctgtcct ctgagcacta cagcagccaa
1141 tcccatggca acagcatgac tgagctgaag cccagcagca aagcacatct caccaagctg
1201 aaaatacctt cccaaccact ggatgcatca gcttctggtg atgtgagctg tgtggatgaa
1261 atcctaaaag agatgacgca ttcatggcct ccccctctaa cggctattca tacaccatgc
1321 aaaacagaac cttccaaatt tccttttcca actaaggagt ctcagcagtc caattttggc
1381 actggagaac aaaaaagata taatccttct aaaacttcaa atgggcacca gtctaaatct
1441 atgttaaaag atgacttaaa actaagcagc agtgaagaca gtgatgggga acaggattgt
1501 gataagacaa tgccgaggag tacaccagga agtaactctg aaccttcaca ccataatagt
1561 gaaggagcag ataactccag ggatgattct agtagccaca gtggatctga aagcagctct
1621 ggatctgact cagagagtga aagtagttcc agtgacagtg aggcaaatga gccatcccag
1681 agtgcatctc ccgagcctga acccccgcca acaaacaaat ggcaacttga taattggctg
1741 aataaagtga acccacataa agtgtcaccc gcctcttcag tggacagtaa catcccatca
1801 tctcaaggct acaaaaagga aggccgagag cagggcactg ggaatagcta cactgataca
1861 agtggaccta aagaaacgag ttccgctact ccgggacgag actccaaaac catccaaaag
1921 ggatcagaaa gtgggcgtgg gaggcagaaa tctcctgcac agagtgacag cacaacacag
1981 agaagaactg taggcaaaaa acaacccaaa aaggctgaga aggcagctgc tgaagagcct
2041 cgtggaggcc tgaagataga aagtgaaacc cctgtagact tggctagcag catgccctcc
2101 agcagacaca aagcagccac caaaggctca aggaaaccca atataaagaa ggagtctaag
2161 tcttcccctc gacctacagc agagaaaaag aaatataagt caacaagtaa atcttcccag
2221 aaatcaaggg aaatcataga aacagatacc tcatcctcag attcagatga aagtgagagc
2281 cttcctcctt cctcacaaac tcctaagtac cccgagagca ataggactcc tgttaaaccc
2341 tcctcagtgg aggaagaaga tagctttttt cggcaacgaa tgttctctcc tatggaagag
2401 aaggaacttc tttcacccct cagtgagcct gatgacaggt acccacttat tgtgaagatt
2461 gacctgaatc ttttgactag aataccagga aagccttaca aagaaacaga gccgcccaag
2521 ggggaaaaga aaaatgtgcc agaaaagcac acgagagagg ctcagaaaca agcctcagaa
2581 aaagtttcca acaaaggcaa gaggaagcat aagaatgaag atgataaccg agccagtgag
2641 agcaagaaac ccaaaacgga ggacaagaat tcagcaggcc ataagccatc cagcaacaga
2701 gagtcatcta agcagagtgc tgcaaaagaa aaggatttgt tgccttctcc cgctgggcct
2761 gttccttcaa aagatccaaa aacagagcat ggctctcgga agaggactat tagtcagtct
2821 tcttccttaa agtcaagcag taacagcaac aaggagacga gtggcagcag caaaaacagt
2881 tcctccacat caaagcagaa gaagaccgaa gggaagactt ccagtagctc caaggaggtt
2941 aaggaaaagg ctccaagtag ctcctctaac tgtcctccat ctgcaccaac tcttgattct
3001 tctaagcctc ggagaacaaa gcttgtcttt gatgacagaa attattcagc agaccattat
3061 ttacaagaag caaaaaagct aaagcacaat gcagatgcat tgtctgatag gtttgagaaa
3121 gctgtatact atcttgatgc tgtggtatct ttcattgaat gtgggaatgc attagagaag
3181 aatgctcagg aatccaaatc cccattccct atgtattcag agacggtgga tctcatcaaa
3241 tacactatga agctaaagaa ttacttggca ccagatgcta cagctgcaga taaacgactc
3301 acagtacttt gcctgcgatg cgagtctttg ctgtacctga ggctgttcaa actgaagaag
3361 gaaaatgctc tgaagtactc aaagacactg acagagcacc tgaagaattc ttataataat
3421 tctcaagcac catcgcctgg cttgggaagc aaagctgtgg ggatgccttc ccctgtttct
3481 ccaaagctgt caccaggcaa ttcaggaaat tattcatctg gggccagtag tgcttctgca
3541 agtggttctt cagtgaccat tccacagaag atccaccaga tggcagccag ctatgttcag
3601 gtcacatcca acttcctcta tgccaccgaa atttgggacc aagctgaaca gctttccaaa
3661 gagcaaaaag aattctttgc tgaactggat aaagtaatgg gccctctcat ctttaatgca
3721 agcatcatga cagatctagt tcgttatacc cggcagggac tgcactggct tcgccaggat
3781 gccaagttga tatcttgaac tgaacacatt ctcgttgcct ctgattttct ccacaacact
3841 gtgtcacatc acgaaggaaa actgccataa cataccacct agtcgacact aagaatgagg
3901 aatagttttc tcctcgttgg ttcatgtgtt gttgtttttg ataatccaaa gcgatcatgt
3961 cagttggccc tttaatattt ccaatgtgaa agattattta aatgctttta aatctgcagc
4021 acattgataa gatggtttcc gtgagctatg ataagattga aattccagtt gcaattcata
4081 actaatgaat tgaaatttta tttattttaa tttttttaag ttcccagatt ggggctagtt
4141 taagacttag ttatttctgc ctataaattt actctgttga atatttagca taaatttaat
4201 gtagacattt ttatttatat accttgggag ctttattacc taatatcttt ttgtggtagc
4261 tgttctaaga gtaagaattg gttcgtctaa aataaggaca tattctttct acacctgcta
4321 cctgacctga tatttgaaat cttaactcca attttattat tgtggaattt acagttagta
4381 ttaaggatat tcttctacac atgaaaaaat cacttaaata tgagaaaatg gcattctaca
4441 tgtatgtact atgaaatgtg tctgatttgg tatgcattat ttcaaaaact gtgaatgtgt
4501 gttaataaca ctacagcaat cacacagtga ctatagcttg catttaatgc attacaaaag
4561 agggaggaca aactttctag aagaagcatt tcatgttgaa gggtttcata aaaggacaat
4621 cctgaacgtt acggatttta ttaaaaccat atgttatttt ttgttgctaa tcatttgaca
4681 cttaatcgaa acagaataag ttactgtgga aaaaaggatg atccaactcc tgaggattgc
4741 cgcctgtgtg tctttaattg attatctttg atagttagag ttatttttca gatttttccc
4801 cctcatactg gattgtgatg ctctgatatg agcatgcaat gactgataca gaaataggct
4861 ggagcttcgg tgtgtgcttt tgctatacag cttacagttc taatgcctga tgtagagatg
4921 atgaatttct cctttttaaa cctatcttct gtttactcct tttgcattat gaaggccctc
4981 ttcaatgatg tatgtttctg atggaatcag aaaatttcac actagaaaag tgtacctaac
5041 ttgattcagt gtgagctctt gggacaggga gtggagccag gggtagagct taaagaagct
5101 ggctctgtaa gagttgtata tcaaagtacc ttttgctaca aggtgcatat agaagtctaa
5161 gataagaaat gtaccctcta tactttattt ttcatgcccc tatcatgttt ggtgtgcctt
5221 aaatcttacc tttaaatgaa gactctatgg attgtttaca aatgaagttt tacactagga
5281 ccagatcgtc catacatgat tggtcttcta caataataat gttcaattct ttcattttaa
5341 atctttcttt agggtcatat aaagattagt tctttcagaa aagctattgc tttggtacat
5401 gacttagtct aaccagttgg gtaatgctgc tttgaaaaat agtttgtccc tctccccatt
5461 ccaaaaatga atccattcag tcagaaaaaa agattatcct actatttgta gatgattgct
5521 aaggcttgaa ataggaaagg ggaaagaagg gatagaaaag tttcaagtcc agtagtgcag
5581 tgaatccttt gtgtttgaga cttttttaca cttatttctg aagttagtga actttcattg
5641 tccctgtggc ctcatattta tagagcttct taactgtttt gtacctcaga ttgtttttag
5701 atgtcattga agattgagat gtacaaatta tgttgaattt aacctgtttc tcttggagaa
5761 attgcctctg atatctttct aacttaatct tagtgtgttc taggaacagt tttccataag
5821 aggacaactt ctcaatgatc atgatagtta tttagtaaga aataatggtt gttaatcaaa
5881 tacatgttat gtcttgtcct tctctgattt ttctgacttg gattccttaa tttgcagtga
5941 tgtcttagaa atggaaaaag tatcctctgg gctttcagac tttcagcaac aacagccagt
6001 gggatacctc tgagttcttg tgttctagtc tcttgtatgt cctgaaaggc agcaggagca
6061 tttttggggt cacactgata ttttgttaaa gcagagtttc ctgcatggtt tatggtgctg
6121 ctctaatatt ttctctttaa aactgtgtca atttttggag aatgaatttt tcaacctttt
6181 cattgtggag gatttttccc acaaggtagt aatgggcagt ggatcaaaat cctatcataa
6241 atgattgaat tttcaatgtc atagtgtgtg gaaatactac tccctttcct ccatgtctat
6301 cttcttagat gttctgtctt tgtcatcatc tgctccagca catactttgg actcctttac
6361 aggtgacctt cccccctccc tcctcaatag gtgtcctcag tgttttctaa gcatttaaaa
6421 ctgaatttga caagtggcta aaaacttagt ctgagacttt tacttagtat atggttaatt
6481 atagaaacaa caactatttt aaacagttac tctgttgact gctctttatg actacacttg
6541 tatgccagga ggcagcagcc tatacaccat tagactcttt acaagcaacc aacgaaccaa
6601 ttgcagtggt tgcagttgtc acagtagtct tgcctgggcc agactgggct gggctgtggg
6661 aagcattccc cttcagggga gtttccacag ctcagcccag gctccagtct ggctagagag
6721 catccagccc attccttgcc tcagtgccac ctttttctgg gcaagtagcc aagaagtgac
6781 tttatatgca gtgagtactt cagataaact tacagctaca gctgccgaag agtggagaag
6841 gttgaccacc atttatttaa ttctctagtg taaaaacaat tgagagcagt gtgcgtgagc
6901 ccacagacac ctgtgtttct gggagctctg gca
//