LOCUS BC136563 2690 bp mRNA linear HUM 12-MAY-2008
DEFINITION Homo sapiens spondin 1, extracellular matrix protein, mRNA (cDNA
clone MGC:168176 IMAGE:9020553), complete cds.
ACCESSION BC136563
VERSION BC136563.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2690)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2690)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 670 Row: n Column: 7.
FEATURES Location/Qualifiers
source 1..2690
/db_xref="H-InvDB:HIT000500576"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:168176 IMAGE:9020553"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_362"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CCCCATCC"
gene 1..2690
/gene="SPON1"
/gene_synonym="f-spondin"
/db_xref="GeneID:10418"
/db_xref="HGNC:HGNC:11252"
/db_xref="MIM:604989"
CDS 211..2634
/gene="SPON1"
/gene_synonym="f-spondin"
/codon_start=1
/product="spondin 1, extracellular matrix protein"
/protein_id="AAI36564.1"
/db_xref="GeneID:10418"
/db_xref="HGNC:HGNC:11252"
/db_xref="MIM:604989"
/translation="MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYC
SRILRAQGTRREGYTEFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENR
EGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILK
ASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEK
THPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQS
DEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAED
LCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDP
EGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSP
WSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWI
TWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEECSPSSCLMTEWGE
WDECSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKCMMPECHTIPCLLSPWSEWS
DCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPECPIDCELTEWSQWSECNK
SCGKGHVIRTRMIQMEPQFGGAPCPETVQRKKCRIRKCLRNPSIQKLRWREARESRRS
EQLKEESEGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTVKKRFKSSQFTSCKDKKE
IRACNVHPC"
BASE COUNT 659 a 764 c 779 g 488 t
ORIGIN
1 ctctcgttcg cttggctcag ctcagctcag ctcagcgcag ctccgcggcc gccaagccga
61 ggcgggcacg gtctccgagt cgcggacgcc agctccgagc tccctctctc cgccgcgcct
121 ccgccaggtc gcgccttcgt cgggaccact tcgggcagga gtcgcgtggc gaaggcctgc
181 ggccgcgaca caaagttggg ggccgcgaag atgaggctgt ccccggcgcc cctgaagctg
241 agccggactc cggcactgct ggccctggcg ctgcccctgg ccgcggcgct ggccttctcc
301 gacgagaccc tggacaaagt gcccaagtca gagggctact gcagccgtat cctgcgcgcc
361 cagggcacgc ggcgcgaggg ctacaccgag ttcagcctcc gcgtggaggg cgaccccgac
421 ttctacaagc cgggaaccag ctaccgcgta acactttcag ctgctcctcc ctcctacttc
481 agaggattca cattaattgc cctcagagag aacagagagg gtgataagga agaagaccat
541 gctgggacct tccagatcat agacgaagaa gaaactcagt ttatgagcaa ttgccctgtt
601 gcagtcactg aaagcactcc acggaggagg acccggatcc aggtgttttg gatagcacca
661 ccagcgggaa caggctgcgt gattctgaag gccagcatcg tacaaaaacg cattatttat
721 tttcaagatg agggctctct gaccaagaaa ctttgtgaac aagattccac atttgatggg
781 gtgactgaca aacccatctt agactgctgt gcctgcggaa ctgccaagta cagactcaca
841 ttttatggga attggtccga gaagacacac ccaaaggatt accctcgtcg ggccaaccac
901 tggtctgcga tcatcggagg atcccactcc aagaattatg tactgtggga atatggagga
961 tatgccagcg aaggcgtcaa acaagttgca gaattgggct cacccgtgaa aatggaggaa
1021 gaaattcgac aacagagtga tgaggtcctc accgtcatca aagccaaagc ccagtggcca
1081 gcctggcagc ctctcaacgt gagagcagca ccttcagctg aattttccgt ggacagaacg
1141 cgccatttaa tgtccttcct gaccatgatg ggccctagtc ccgactggaa cgtaggctta
1201 tctgcagaag atctgtgcac caaggaatgt ggctgggtcc agaaggtggt gcaagacctg
1261 attccctggg acgctggcac cgacagcggg gtgacctatg agtcacccaa caaacccacc
1321 attccccagg agaaaatccg gcccctgacc agcctggacc atcctcagag tcctttctat
1381 gacccagagg gtgggtccat cactcaagta gccagagttg tcatcgagag aatcgcacgg
1441 aagggtgaac aatgcaatat tgtacctgac aatgtcgatg atattgtagc tgacctggct
1501 ccagaagaga aagatgaaga tgacacccct gaaacctgca tctactccaa ctggtcccca
1561 tggtccgcct gcagctcctc cacctgtgac aaaggcaaga ggatgcgaca gcgcatgctg
1621 aaagcacagc tggacctcag cgtcccctgc cctgacaccc aggacttcca gccctgcatg
1681 ggccctggct gcagtgacga agacggctcc acctgcacca tgtccgagtg gatcacctgg
1741 tcgccctgca gcatctcctg cggcatgggc atgaggtccc gggagaggta tgtgaagcag
1801 ttcccggagg acggctccgt gtgcacgctg cccactgagg aaacggagaa gtgcacggtc
1861 aacgaggagt gctctcccag cagctgcctg atgaccgagt ggggcgagtg ggacgagtgc
1921 agcgccacct gcggcatggg catgaagaag cggcaccgca tgatcaagat gaaccccgca
1981 gatggctcca tgtgcaaagc cgagacatca caggcagaga agtgcatgat gccagagtgc
2041 cacaccatcc catgcttgct gtccccatgg tccgagtgga gtgactgcag cgtgacctgc
2101 gggaagggca tgcgaacccg acagcggatg ctcaagtctc tggcagaact tggagactgc
2161 aatgaggatc tggagcaggt ggagaagtgc atgctccctg aatgccccat tgactgtgag
2221 ctcaccgagt ggtcccagtg gtcggaatgt aacaagtcat gtgggaaagg ccacgtgatt
2281 cgaacccgga tgatccaaat ggagcctcag tttggaggtg caccctgccc agagactgtg
2341 cagcgaaaaa agtgccgcat ccgaaaatgc cttcgaaatc catccatcca aaagctacgc
2401 tggagggagg cccgagagag ccggcggagt gagcagctga aggaagagtc tgaaggggag
2461 cagttcccag gttgtaggat gcgcccatgg acggcctggt cagaatgcac caaactgtgc
2521 ggaggtggaa ttcaggaacg ttacatgact gtaaagaaga gattcaaaag ctcccagttt
2581 accagctgca aagacaagaa ggagatcaga gcatgcaatg ttcatccttg ttagcaaggg
2641 tacgagttcc ccagggctgc actctagatt ccagagtcac caatggctgg
//