LOCUS BC136553 3289 bp mRNA linear HUM 11-FEB-2009
DEFINITION Homo sapiens discs, large homolog 4 (Drosophila), mRNA (cDNA clone
MGC:168166 IMAGE:9020543), complete cds.
ACCESSION BC136553
VERSION BC136553.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3289)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3289)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Ambion
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 670 Row: m Column: 21
single stranded.
FEATURES Location/Qualifiers
source 1..3289
/db_xref="H-InvDB:HIT000500566"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:168166 IMAGE:9020543"
/tissue_type="Testicle, PCR rescued clones"
/clone_lib="NIH_MGC_382"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: GTAAGTTA"
gene 1..3289
/gene="DLG4"
/gene_synonym="PSD95"
/gene_synonym="SAP-90"
/gene_synonym="SAP90"
/db_xref="GeneID:1742"
/db_xref="HGNC:HGNC:2903"
/db_xref="MIM:602887"
CDS 735..3029
/gene="DLG4"
/gene_synonym="PSD95"
/gene_synonym="SAP-90"
/gene_synonym="SAP90"
/codon_start=1
/product="DLG4 protein"
/protein_id="AAI36554.1"
/db_xref="GeneID:1742"
/db_xref="HGNC:HGNC:2903"
/db_xref="MIM:602887"
/translation="MSQRPRAPRSALWLLAPPLLRWAPPLLTVLHSDLFQALLDILDY
YEASLSESQKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYVNGTEGEME
YEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFV
NEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGV
GNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTY
DVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRY
SPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGE
LRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQ
LMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDASDE
EWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETV
TQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVS
SREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAH
LHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEI
YHKVKRVIEDLSGPYIWVPARERL"
BASE COUNT 742 a 951 c 947 g 649 t
ORIGIN
1 ggcagaggca gagacatgga aagacagact ctagggttcc tgatgaaatc tatctcggcc
61 aacacaaaag ggagggtaca gtggtggggg gcacccaagc tagggtgtga gtaccctaag
121 tgtattcttc tgagatgtag gccattcact aactcttgga acagctacag tttcacagta
181 ggaagacccc cccagattca ctgcccctcc cttagtaaag cctctgagac cttcctgaac
241 attcccttct gtctttgccc tctgttcctt ccagagacca tgtgcccagg cagatggatt
301 cctcccgggc ctgagaggaa ctgcaggaat tctcctgcct cttacccgta aaaccccaac
361 ttctctagcc ctagggcagg aagtcccaaa caatttctac ccctttttct gcaattctca
421 ttggggtgag aggaggccca ggaggagaga gagctgggct cagcttcttt ttgagctgct
481 ggagccctct gtgaggaggc cctctttgct ggcttctcag gagagcgtgg ctaggttctg
541 cctgcctatg ggaagagggg gccagggtgt gtggagcaag atggtggcgg tgctggtgcc
601 ttgggacctg ggggaatggg acagctggtc ggctcagaga cggcctactt tactcacagc
661 tggaatttag tggggagaag cagctcaact ccaatcctgg aggattaggg agattaaagt
721 gagagaagag agagatgtcc cagagaccaa gagctcccag gtcagccctc tggctcctgg
781 cacccccact gctgcggtgg gcacccccac tcctcacagt gctgcatagc gacctcttcc
841 aggccttgct ggacatcctg gactattatg aggcttccct ctcagagagt cagaaatacc
901 gctaccaaga tgaagacacg ccccctctgg agcacagccc ggcccacctc cccaaccagg
961 ccaattctcc cccagtgatt gtcaacacag ataccctaga agccccagga tatgtgaacg
1021 ggaccgaggg ggagatggaa tacgaggaaa tcacattgga aaggggtaac tcaggtctgg
1081 gcttcagcat cgcaggtggc actgacaacc cacacatcgg tgacgaccca tccattttca
1141 tcaccaagat cattcctggt ggggctgcgg cccaggatgg ccgcctcagg gtcaacgaca
1201 gcatcctgtt tgtaaatgaa gtggacgtgc gcgaggtgac ccactcagcg gcggtggaag
1261 ccctcaaaga ggcaggctcc atcgttcgcc tctatgtcat gcgccggaag cccccggctg
1321 agaaggtcat ggagatcaag ctcatcaagg ggcctaaagg tcttggcttc agcatcgcag
1381 ggggcgtagg gaaccagcac atcccaggag ataatagcat ctatgtaaca aagatcatcg
1441 aagggggtgc tgcccacaag gatgggaggt tgcagattgg agacaagatc ctggcggtca
1501 acagtgtggg gctagaggac gtcatgcatg aagatgctgt ggcagccctg aagaacacgt
1561 atgatgttgt ctacctaaag gtggccaagc ccagcaatgc ctacctgagt gacagctatg
1621 ctcccccaga catcacaacc tcttattccc agcacctgga caatgagatc agtcacagca
1681 gctacctggg caccgactac cccacagcca tgacccccac ttcccctcgg cgctactctc
1741 cagtggccaa ggacctgctc ggggaggaag acattccccg agaaccgagg cgaattgtga
1801 tccaccgggg ctccacgggc ctgggcttca acatcgtggg tggcgaggac ggtgaaggca
1861 tcttcatctc ctttatcctg gccgggggcc ctgcagacct cagtggggag ctgcggaagg
1921 gggaccagat cctgtcggtc aacggtgtgg acctccgaaa tgccagccat gagcaggctg
1981 ccattgccct gaagaatgcg ggtcagacgg tcacgatcat cgctcagtat aaaccagaag
2041 agtacagccg attcgaggcc aagatccacg accttcggga acagctcatg aacagcagcc
2101 tgggctcagg gactgcgtcc ctgcggagca accccaaaag gggtttctac atcagggccc
2161 tgtttgatta cgacaagacc aaggactgcg gcttcctgag ccaggccctg agcttccgct
2221 ttggggatgt gctgcatgtc atcgatgcta gtgatgagga gtggtggcag gcacggcggg
2281 tccactctga cagtgagacc gacgacattg ggttcatccc cagcaaacgg cgggttgagc
2341 gacgagagtg gtcaaggtta aaggccaagg actggggctc cagctctgga tcgcagggtc
2401 gagaagactc ggttctgagc tacgagacag tgacgcagat ggaagtgcac tatgctcgcc
2461 ccatcatcat ccttgggccc accaaggacc gcgccaacga tgatcttctc tccgagttcc
2521 ccgacaagtt tggatcctgt gttccccata cgacacggcc caagcgggag tatgagatag
2581 atggccggga ttaccacttt gtgtcgtccc gggagaaaat ggagaaggac attcaggcgc
2641 acaagttcat tgaggccggc cagtacaaca gccacctcta tgggaccagc gtccagtccg
2701 tgcgagaggt ggcagagcag gggaagcact gcatcctcga tgtctcggcc aatgccgtgc
2761 ggcggctgca ggcggcccac ctgcacccca tcgccatctt catccgcccc cgctccctgg
2821 agaatgtgct agagattaac aagcggatca cagaggagca agcccgcaaa gccttcgaca
2881 gagccaccaa gctggagcag gagttcacag agtgcttctc agccatcgtg gagggtgaca
2941 gctttgagga gatctaccac aaggtgaagc gtgtcatcga ggacctctca ggcccctaca
3001 tctgggttcc agcccgagag agactctgat tcctgccctg gcttggcctg gactcgccct
3061 gcctccatca cctgggccct tggtctggac tgaattgccc aagcccttgg ctccccccgg
3121 cctccctccc accccttctt atttatttcc tttctaactg gatccagcct gttggagggg
3181 ggacactcct ctgcatgtat ccccgcaccc cagaactggg ctcctgaacg ccaggaacct
3241 ggggtctggg ggggagctgg gctccttgtt ccgagccctt gctccttag
//