LOCUS BC136486 2450 bp mRNA linear HUM 11-FEB-2009
DEFINITION Homo sapiens proprotein convertase subtilisin/kexin type 1, mRNA
(cDNA clone MGC:168098 IMAGE:9020475), complete cds.
ACCESSION BC136486
VERSION BC136486.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2450)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2450)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Ambion
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 670 Row: k Column: 1
single stranded.
FEATURES Location/Qualifiers
source 1..2450
/db_xref="H-InvDB:HIT000500503"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:168098 IMAGE:9020475"
/tissue_type="Testicle, PCR rescued clones"
/clone_lib="NIH_MGC_382"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: GCAAGCCG"
gene 1..2450
/gene="PCSK1"
/gene_synonym="BMIQ12"
/gene_synonym="PC1"
/gene_synonym="PC3"
/gene_synonym="SPC3"
/db_xref="GeneID:5122"
/db_xref="HGNC:HGNC:8743"
/db_xref="MIM:162150"
CDS 122..2383
/gene="PCSK1"
/gene_synonym="BMIQ12"
/gene_synonym="PC1"
/gene_synonym="PC3"
/gene_synonym="SPC3"
/codon_start=1
/product="proprotein convertase subtilisin/kexin type 1"
/protein_id="AAI36487.1"
/db_xref="GeneID:5122"
/db_xref="HGNC:HGNC:8743"
/db_xref="MIM:162150"
/translation="MERRAWSLQCTAFVLFCAWCALNSAKAKRQFVNEWAAEIPGGPE
AASAIAEELGYDLLGQIGSLENHYLFKHKNHPRRSRRSAFHITKRLSDDDRVIWAEQQ
YEKERSKRSALRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKG
VVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAM
QANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKT
VEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSA
SQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIF
ALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALV
DLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQ
FEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGT
WTLRITDMSGRIQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMV
DPGEEQPTQENPKENTLVSKSPSSSSVGGRRDELEEGAPSEAMLRLLQSAFSKNSPPK
QSPKKSPTAKLNIPYENFYEALEKLNKPSQLKDSEDSLYNDYVDVFYNTKPYKHRDDR
LLQALVDILNEEN"
BASE COUNT 688 a 562 c 641 g 559 t
ORIGIN
1 accaggagcc aggagtggtc cagagcccga gggtgggaag ggggagtctg tctggctttt
61 ctcctatctt gcttcttttt cctcttccct tcccactctt gttcaagcga gtgtgtgagc
121 tatggagcga agagcctgga gtctgcagtg cactgctttc gtcctctttt gcgcttggtg
181 tgcactgaac agtgcaaaag cgaaaaggca atttgtcaat gaatgggcag cggagatccc
241 cgggggcccg gaagcagcct cggccatcgc cgaggagctg ggctatgacc ttttgggtca
301 gattggttca cttgaaaatc actacttatt caaacataaa aaccacccca gaaggtctcg
361 aaggagtgcc tttcatatca ctaagagatt atctgatgat gatcgtgtga tatgggctga
421 acaacagtat gaaaaagaaa gaagtaaacg ttcagctcta agggactcag cactaaatct
481 cttcaatgat cccatgtgga atcagcaatg gtacttgcaa gataccagga tgacggcagc
541 cctgcccaag ctggaccttc atgtgatacc tgtttggcaa aaaggcatta cgggcaaagg
601 agttgttatc accgtactgg atgatggttt ggagtggaat cacacggaca tttatgccaa
661 ctatgatcca gaggctagct atgattttaa tgataatgac catgatccat ttccccgata
721 tgatcccaca aacgagaaca aacacgggac cagatgtgca ggagaaattg ccatgcaagc
781 aaataatcac aaatgcgggg ttggagttgc atacaattcc aaagttggag gcataagaat
841 gctggatggc attgtgacgg atgctattga ggccagttca attggattca atcctggaca
901 cgtggatatt tacagtgcaa gctggggccc taatgatgat gggaaaactg tggaggggcc
961 tggccggcta gcccagaagg cttttgaata tggtgtcaaa caggggagac aggggaaggg
1021 gtccatcttc gtctgggctt cgggaaacgg ggggcgtcag ggagataatt gtgactgtga
1081 tggctacaca gacagcatct acaccatctc catcagcagt gcctcccaac aaggcctatc
1141 cccctggtac gctgagaagt gctcctccac actggccacc tcttacagca gcggagatta
1201 caccgaccag agaatcacga gcgctgacct gcacaatgac tgcacggaga cgcacacagg
1261 cacctcggcc tctgcacctc tggctgctgg catcttcgct ctggccctgg aagcaaaccc
1321 aaatctcacc tggcgagata tgcagcacct ggttgtctgg acctctgagt atgacccgct
1381 ggccaataac cctggatgga aaaagaatgg agcaggcttg atggtgaata gtcgatttgg
1441 atttggcttg ctaaatgcca aagctctggt ggatttagct gaccccagga cctggaggag
1501 cgtgcctgag aagaaagagt gtgttgtaaa ggacaatgac tttgagccca gagccctgaa
1561 agctaatgga gaagttatca ttgaaattcc aacaagagct tgtgaaggac aagaaaatgc
1621 tatcaagtcc ctggagcatg tacaatttga agcaacaatt gaatattccc gaagaggaga
1681 ccttcatgtc acacttactt ctgctgctgg aactagcact gtgctcttgg ctgaaagaga
1741 acgggataca tctcctaatg gctttaagaa ttgggacttc atgtctgttc acacatgggg
1801 agagaaccct ataggtactt ggactttgag aattacagac atgtctggaa gaattcaaaa
1861 tgaaggaaga attgtgaact ggaagctgat tttgcacggg acctcttctc agccagagca
1921 tatgaagcag cctcgtgtgt acacgtccta caacactgtt cagaatgaca gaagaggggt
1981 ggagaagatg gtggatccag gggaggagca gcccacacaa gagaacccta aggagaacac
2041 cctggtgtcc aaaagcccca gcagcagcag cgtagggggc cggagggatg agttggagga
2101 gggagcccct tccgaggcca tgctgcgact cctgcaaagt gctttcagta aaaactcacc
2161 gccaaagcaa tcaccaaaga agtccccaac tgcaaagctc aacatccctt atgaaaactt
2221 ctacgaagcc ctggaaaagc tgaacaaacc ttcccagctt aaagactctg aagacagtct
2281 gtataatgac tatgttgatg ttttttataa cactaaacct tacaagcaca gagacgaccg
2341 gctgcttcaa gctctggtgg acattctgaa tgaggaaaat taaaataagt gtgtggtccc
2401 aagttggaaa tattcatgct tcttccttac cctgcgattt tgcctgtgtc
//