LOCUS BC136469 4952 bp mRNA linear HUM 11-FEB-2009
DEFINITION Homo sapiens thrombospondin 1, mRNA (cDNA clone MGC:168081
IMAGE:9020458), complete cds.
ACCESSION BC136469
VERSION BC136469.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4952)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4952)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Ambion
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 670 Row: j Column: 8
single stranded.
FEATURES Location/Qualifiers
source 1..4952
/db_xref="H-InvDB:HIT000500486"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:168081 IMAGE:9020458"
/tissue_type="Testicle, PCR rescued clones"
/clone_lib="NIH_MGC_382"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: AGAGACTC"
gene 1..4952
/gene="THBS1"
/gene_synonym="THBS"
/gene_synonym="THBS-1"
/gene_synonym="TSP"
/gene_synonym="TSP1"
/db_xref="GeneID:7057"
/db_xref="HGNC:HGNC:11785"
/db_xref="MIM:188060"
CDS 154..3666
/gene="THBS1"
/gene_synonym="THBS"
/gene_synonym="THBS-1"
/gene_synonym="TSP"
/gene_synonym="TSP1"
/codon_start=1
/product="thrombospondin 1"
/protein_id="AAI36470.1"
/db_xref="GeneID:7057"
/db_xref="HGNC:HGNC:11785"
/db_xref="MIM:188060"
/translation="MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGS
GRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRG
TLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFV
QEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFV
FGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDE
LSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVD
SCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCS
TSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVT
CGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTC
GGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYP
DGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRF
TGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGY
AGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDD
DDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACA
ADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTC
DNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVP
NPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQ
NDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQ
SSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPG
QVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGG
RLGLFVFSQEMVFFSDLKYECRDP"
BASE COUNT 1277 a 1296 c 1274 g 1105 t
ORIGIN
1 agttcagggc tcctgtcgct ctccaggagc aacctctact ccggacgcac aggcattccc
61 cgcgcccctc cagccctcgc cgccctcgcc accgctcccg gccgccgcgc tccggtacac
121 acaggatccc tgctgggcac caacagctcc accatggggc tggcctgggg actaggcgtc
181 ctgttcctga tgcatgtgtg tggcaccaac cgcattccag agtctggcgg agacaacagc
241 gtgtttgaca tctttgaact caccggggcc gcccgcaagg ggtctgggcg ccgactggtg
301 aagggccccg acccttccag cccagctttc cgcatcgagg atgccaacct gatcccccct
361 gtgcctgatg acaagttcca agacctggtg gatgctgtgc gggcagaaaa gggtttcctc
421 cttctggcat ccctgaggca gatgaagaag acccggggca cgctgctggc cctggagcgg
481 aaagaccact ctggccaggt cttcagcgtg gtgtccaatg gcaaggcggg caccctggac
541 ctcagcctga ccgtccaagg aaagcagcac gtggtgtctg tggaagaagc tctcctggca
601 accggccagt ggaagagcat caccctgttt gtgcaggaag acagggccca gctgtacatc
661 gactgtgaaa agatggagaa tgctgagttg gacgtcccca tccaaagcgt cttcaccaga
721 gacctggcca gcatcgccag actccgcatc gcaaaggggg gcgtcaatga caatttccag
781 ggggtgctgc agaatgtgag gtttgtcttt ggaaccacac cagaagacat cctcaggaac
841 aaaggctgct ccagctctac cagtgtcctc ctcacccttg acaacaacgt ggtgaatggt
901 tccagccctg ccatccgcac taactacatt ggccacaaga caaaggactt gcaagccatc
961 tgcggcatct cctgtgatga gctgtccagc atggtcctgg aactcagggg cctgcgcacc
1021 attgtgacca cgctgcagga cagcatccgc aaagtgactg aagagaacaa agagttggcc
1081 aatgagctga ggcggcctcc cctatgctat cacaacggag ttcagtacag aaataacgag
1141 gaatggactg ttgatagctg cactgagtgt cactgtcaga actcagttac catctgcaaa
1201 aaggtgtcct gccccatcat gccctgctcc aatgccacag ttcctgatgg agaatgctgt
1261 cctcgctgtt ggcccagcga ctctgcggac gatggctggt ctccatggtc cgagtggacc
1321 tcctgttcta cgagctgtgg caatggaatt cagcagcgcg gccgctcctg cgatagcctc
1381 aacaaccgat gtgagggctc ctcggtccag acacggacct gccacattca ggagtgtgac
1441 aagagattta aacaggatgg tggctggagc cactggtccc cgtggtcatc ttgttctgtg
1501 acatgtggtg atggtgtgat cacaaggatc cggctctgca actctcccag cccccagatg
1561 aacgggaaac cctgtgaagg cgaagcgcgg gagaccaaag cctgcaagaa agacgcctgc
1621 cccatcaatg gaggctgggg tccttggtca ccatgggaca tctgttctgt cacctgtgga
1681 ggaggggtac agaaacgtag tcgtctctgc aacaacccca caccccagtt tggaggcaag
1741 gactgcgttg gtgatgtaac agaaaaccag atctgcaaca agcaggactg tccaattgat
1801 ggatgcctgt ccaatccctg ctttgccggc gtgaagtgta ctagctaccc tgatggcagc
1861 tggaaatgtg gtgcttgtcc ccctggttac agtggaaatg gcatccagtg cacagatgtt
1921 gatgagtgca aagaagtgcc tgatgcctgc ttcaaccaca atggagagca ccggtgtgag
1981 aacacggacc ccggctacaa ctgcctgccc tgccccccac gcttcaccgg ctcacagccc
2041 ttcggccagg gtgtcgaaca tgccacggcc aacaaacagg tgtgcaagcc ccgtaacccc
2101 tgcacggatg ggacccacga ctgcaacaag aacgccaagt gcaactacct gggccactat
2161 agcgacccca tgtaccgctg cgagtgcaag cctggctacg ctggcaatgg catcatctgc
2221 ggggaggaca cagacctgga tggctggccc aatgagaacc tggtgtgcgt ggccaatgcg
2281 acttaccact gcaaaaagga taattgcccc aaccttccca actcagggca ggaagactat
2341 gacaaggatg gaattggtga tgcctgtgat gatgacgatg acaatgataa aattccagat
2401 gacagggaca actgtccatt ccattacaac ccagctcagt atgactatga cagagatgat
2461 gtgggagacc gctgtgacaa ctgtccctac aaccacaacc cagatcaggc agacacagac
2521 aacaatgggg aaggagacgc ctgtgctgca gacattgatg gagacggtat cctcaatgaa
2581 cgggacaact gccagtacgt ctacaatgtg gaccagagag acactgatat ggatggggtt
2641 ggagatcagt gtgacaattg ccccttggaa cacaatccgg atcagctgga ctctgactca
2701 gaccgcattg gagatacctg tgacaacaat caggatattg atgaagatgg ccaccagaac
2761 aatctggaca actgtcccta tgtgcccaat gccaaccagg ctgaccatga caaagatggc
2821 aagggagatg cctgtgacca cgatgatgac aacgatggca ttcctgatga caaggacaac
2881 tgcagactcg tgcccaatcc cgaccagaag gactctgacg gcgatggtcg aggtgatgcc
2941 tgcaaagatg attttgacca tgacagtgtg ccagacatcg atgacatctg tcctgagaat
3001 gttgacatca gtgagaccga tttccgccga ttccagatga ttcctctgga ccccaaaggg
3061 acatcccaaa atgaccctaa ctgggttgta cgccatcagg gtaaagaact cgtccagact
3121 gtcaactgtg atcctggact cgctgtaggt tatgatgagt ttaatgctgt ggacttcagt
3181 ggcaccttct tcatcaacac cgaaagggac gatgactatg ctggatttgt ctttggctac
3241 cagtccagca gccgctttta tgttgtgatg tggaagcaag tcacccagtc ctactgggac
3301 accaacccca cgagggctca gggatactcg ggcctttctg tgaaagttgt aaactccacc
3361 acagggcctg gcgagcacct gcggaacgcc ctgtggcaca caggaaacac ccctggccag
3421 gtgcgcaccc tgtggcatga ccctcgtcac ataggctgga aagatttcac cgcctacaga
3481 tggcgtctca gccacaggcc aaagacgggt ttcattagag tggtgatgta tgaagggaag
3541 aaaatcatgg ctgactcagg acccatctat gataaaacct atgctggtgg tagactaggg
3601 ttgtttgtct tctctcaaga aatggtgttc ttctctgacc tgaaatacga atgtagagat
3661 ccctaatcat caaattgttg attgaaagac tgatcataaa ccaatgctgg tattgcacct
3721 tctggaacta tgggcttgag aaaaccccca ggatcacttc tccttggctt ccttcttttc
3781 tgtgcttgca tcagtgtgga ctcctagaac gtgcgacctg cctcaagaaa atgcagtttt
3841 caaaaacaga ctcagcattc agcctccaat gaataagaca tcttccaagc atataaacaa
3901 ttgctttggt ttccttttga aaaagcatct acttgcttca gttgggaagg tgcccattcc
3961 actctgcctt tgtcacagag cagggtgcta ttgtgaggcc atctctgagc agtggactca
4021 aaagcatttt caggcatgtc agagaaggga ggactcacta gaattagcaa acaaaaccac
4081 cctgacatcc tccttcagga acacggggag cagaggccaa agcactaagg ggagggcgca
4141 tacccgagac gattgtatga agaaaatatg gaggaactgt tacatgttcg gtactaagtc
4201 attttcaggg gattgaaaga ctattgctgg atttcatgat gctgactggc gttagctgat
4261 taacccatgt aaataggcac ttaaatagaa gcaggaaagg gagacaaaga ctggcttctg
4321 gacttcctcc ctgatcccca cccttactca tcacctgcag tggccagaat tagggaatca
4381 gaatcaaacc agtgtaaggc agtgctggct gccattgcct ggtcacattg aaattggtgg
4441 cttcattcta gatgtagctt gtgcagatgt agcaggaaaa taggaaaacc taccatctca
4501 gtgagcacca gctgcctccc aaaggagggg cagccgtgct tatattttta tggttacaat
4561 ggcacaaaat tattatcaac ctaactaaaa cattcctttt ctcttttttc ctgaattatc
4621 atggagtttt ctaattctct cttttggaat gtagattttt tttaaatgct ttacgatgta
4681 aaatatttat tttttactta ttctggaaga tctggctgaa ggattattca tggaacagga
4741 agaagcgtaa agactatcca tgtcatcttt gttgagagtc ttcgtgactg taagattgta
4801 aatacagatt atttattaac tctgttctgc ctggaaattt aggcttcata cggaaagtgt
4861 ttgagagcaa gtagttgaca tttatcagca aatctcttgc aagaacagca caaggaaaat
4921 cagtctaata agctgctctg ccccttgtgc tc
//