LOCUS BC136465 4601 bp mRNA linear HUM 11-FEB-2009
DEFINITION Homo sapiens glutamyl-prolyl-tRNA synthetase, mRNA (cDNA clone
MGC:168077 IMAGE:9020454), complete cds.
ACCESSION BC136465
VERSION BC136465.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4601)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4601)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Ambion
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 670 Row: j Column: 4
single stranded.
FEATURES Location/Qualifiers
source 1..4601
/db_xref="H-InvDB:HIT000500483"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:168077 IMAGE:9020454"
/tissue_type="Testicle, PCR rescued clones"
/clone_lib="NIH_MGC_382"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CCATAGCC"
gene 1..4601
/gene="EPRS"
/gene_synonym="EARS"
/gene_synonym="GLUPRORS"
/gene_synonym="PARS"
/gene_synonym="PIG32"
/db_xref="GeneID:2058"
/db_xref="HGNC:HGNC:3418"
/db_xref="MIM:138295"
CDS 39..4577
/gene="EPRS"
/gene_synonym="EARS"
/gene_synonym="GLUPRORS"
/gene_synonym="PARS"
/gene_synonym="PIG32"
/codon_start=1
/product="glutamyl-prolyl-tRNA synthetase"
/protein_id="AAI36466.1"
/db_xref="GeneID:2058"
/db_xref="HGNC:HGNC:3418"
/db_xref="MIM:138295"
/translation="MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSE
NVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINEL
NHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAF
QSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAA
LLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETI
MKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQ
SCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTH
ALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGW
DDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYV
ALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMV
TFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICV
TYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQ
PYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSL
NNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQE
YKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVEC
LLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEV
VRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKS
EKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQ
VITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALE
KEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQ
WCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIP
VVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIP
GEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDK
EALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQ
FVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKI
LDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAK
CVCGKNPAKYYTLFGRSY"
BASE COUNT 1499 a 872 c 1081 g 1149 t
ORIGIN
1 ccaggcgtac tgacaggtgg accagcggac tggtggagat ggcgacgctc tctctgaccg
61 tgaattcagg agaccctccg ctaggagctt tgctggcagt agaacacgtg aaagacgatg
121 tcagcatttc cgttgaagaa gggaaagaga atattcttca tgtttctgaa aatgtgatat
181 tcacagatgt gaattctata cttcgctact tggctagagt tgcaactaca gctgggttat
241 atggctctaa tctgatggaa catactgaga ttgatcactg gttggagttc agtgctacaa
301 aattatcttc atgtgattcc tttacttcta caattaatga actcaatcat tgcctgtctc
361 tgagaacata cttagttgga aactccttga gtttagcaga tttatgtgtt tgggccaccc
421 taaaaggaaa tgctgcctgg caagaacagt tgaaacagaa gaaagctcca gttcatgtaa
481 aacgttggtt tggctttctt gaagcccagc aggccttcca gtcagtaggt accaagtggg
541 atgtttcaac aaccaaagct cgagtggcac ctgagaaaaa gcaagatgtt gggaaatttg
601 ttgagcttcc aggtgcggag atgggaaagg ttaccgtcag atttcctcca gaggccagtg
661 gttacttaca cattgggcat gcaaaagctg ctcttctgaa ccagcactac caggttaact
721 ttaaagggaa actgatcatg agatttgatg acacaaatcc tgaaaaagaa aaggaagatt
781 ttgagaaggt tatcttggaa gatgttgcaa tgttgcatat caaaccagat caatttactt
841 atacttcgga tcattttgaa actataatga agtatgcaga gaagctaatt caagaaggga
901 aggcttatgt ggatgatact cctgctgaac agatgaaagc agaacgtgag cagaggatag
961 actctaaaca tagaaaaaac cctattgaga agaatctaca aatgtgggaa gaaatgaaaa
1021 aagggagcca gtttggtcag tcctgttgtt tgcgagcaaa aattgacatg agtagtaaca
1081 atggatgcat gagagatcca accctttatc gctgcaaaat tcaaccacat ccaagaactg
1141 gaaataaata caatgtttat ccaacatatg attttgcctg ccccatagtt gacagcatcg
1201 aaggtgttac acatgccctg agaacaacag aataccatga cagagatgag cagttttact
1261 ggattattga agctttaggc ataagaaaac catatatttg ggaatatagt cggctaaatc
1321 tcaacaacac agtgctatcc aaaagaaaac tcacatggtt tgtcaatgaa ggactagtag
1381 atggatggga tgacccaaga tttcctacgg ttcgtggtgt actgagaaga gggatgacag
1441 ttgaaggact gaaacagttt attgctgctc agggctcctc acgttcagtc gtgaacatgg
1501 agtgggacaa aatctgggcg tttaacaaaa aggttattga cccagtggct ccacgatatg
1561 ttgcattact gaagaaagaa gtgatcccag tgaatgtacc tgaagctcag gaggagatga
1621 aagaagtagc caaacaccca aagaatcctg aggttggctt gaagcctgtg tggtatagtc
1681 ccaaagtttt cattgaaggt gctgatgcag agactttttc ggagggtgag atggttacat
1741 ttataaattg gggcaacctc aacattacaa aaatacacaa aaatgcagat ggaaaaatca
1801 tatctcttga tgcaaagttg aatttggaaa acaaagacta caagaaaacc actaaggtca
1861 cttggcttgc agagactaca catgctcttc ctattccagt aatctgtgtc acttatgagc
1921 acttgatcac aaagccagtg ctaggaaaag acgaggactt taagcagtat gtcaacaaga
1981 acagtaagca tgaagagcta atgctagggg atccctgcct taaggatttg aaaaaaggag
2041 atattataca actccagaga agaggattct tcatatgtga tcaaccttat gaacctgtta
2101 gcccatatag ttgcaaggaa gccccgtgtg ttttgatata cattcctgat gggcacacaa
2161 aggaaatgcc aacatcaggg tcaaaggaaa agaccaaagt agaagccaca aaaaatgaga
2221 cctctgctcc ttttaaggaa agaccaacac cttctctgaa taataattgt actacatctg
2281 aggattcctt ggtcctttac aatagagtgg ctgttcaagg agatgtggtt cgtgaattaa
2341 aagccaagaa agcaccaaag gaagatgtag atgcagctgt aaaacagctt ttgtctttga
2401 aagctgaata taaggagaaa actggccagg aatataaacc tggaaaccct cctgctgaaa
2461 taggacagaa tatttcttct aattcctcag caagtattct ggaaagtaaa tctctgtatg
2521 atgaagttgc tgcacaaggg gaggtggttc gtaagctaaa agctgaaaaa tcccctaagg
2581 ctaaaataaa tgaagctgta gaatgcttac tgtccctgaa ggctcagtat aaagaaaaaa
2641 ctgggaagga gtacatacct ggtcagcccc cattatctca aagttcggat tcaagcccaa
2701 ccagaaattc tgaacctgct ggtttagaaa caccagaagc gaaagtactt tttgacaaag
2761 tagcttctca aggggaagta gttcggaaac ttaaaactga aaaagcccct aaggatcaag
2821 tagatatagc tgttcaagaa ctccttcagc taaaggcaca gtacaagtct ttgataggag
2881 tagagtataa gcctgtgtcg gccactggag ctgaggacaa agataagaag aagaaagaaa
2941 aagaaaataa atctgaaaag cagaataagc ctcagaaaca aaatgatggc caaaggaaag
3001 acccttctaa aaaccaagga ggtgggctct catcaagtgg agcaggagaa gggcaggggc
3061 ctaagaaaca gaccaggttg ggtcttgagg caaaaaaaga agaaaatctt gctgattggt
3121 attctcaggt catcacaaag tcagaaatga ttgaatacca tgacataagt ggctgttata
3181 ttcttcgtcc ctgggcctat gccatttggg aagccatcaa ggactttttt gatgctgaga
3241 tcaagaaact tggtgttgaa aactgctact tccccatgtt tgtgtctcaa agtgcattag
3301 agaaagagaa gactcatgtt gccgactttg ccccagaggt tgcttgggtt acaagatctg
3361 gcaaaaccga gctggcagaa ccaattgcca ttcgtcctac tagtgaaaca gtaatgtatc
3421 ctgcatatgc aaaatgggta cagtcacaca gagacctgcc catcaagctc aatcagtggt
3481 gcaatgtggt gcgttgggaa ttcaagcatc ctcagccttt cctacgtact cgtgaatttc
3541 tttggcagga agggcacagt gcttttgcta ccatggaaga ggcagcggaa gaggtcttgc
3601 agatacttga cttatatgct caggtatatg aagaactcct ggcaattcct gttgttaaag
3661 gaagaaagac ggaaaaggaa aaatttgcag gaggagacta tacaactaca atagaagcat
3721 ttatatctgc tagtggaaga gctatccagg gaggaacatc acatcattta gggcagaatt
3781 tttccaaaat gtttgaaatc gtttttgaag atccaaagat accaggagag aagcaatttg
3841 cctatcaaaa ctcctggggc ctgacaactc gaactattgg tgttatgacc atggttcatg
3901 gggacaacat gggtttagta ttaccacccc gtgtagcatg tgttcaggtg gtgattattc
3961 cttgtggcat taccaatgca ctttctgaag aagacaaaga agcgctgatt gcaaaatgca
4021 atgattatcg aaggcgatta ctcagtgtta acatccgcgt tagagctgat ttacgagata
4081 attattctcc aggttggaaa ttcaatcact gggagctcaa gggagttccc attagacttg
4141 aagttgggcc acgtgatatg aagagctgtc agtttgtagc cgtcagacga gatactggag
4201 aaaagctgac agttgctgaa aatgaggcag agactaaact tcaagctatt ttggaagaca
4261 tccaggtcac ccttttcaca agggcttctg aagaccttaa gactcatatg gttgtggcta
4321 atacaatgga agactttcag aagatactag attctggaaa gattgttcag attccattct
4381 gtggggaaat tgactgtgag gactggatca aaaagaccac tgccagggat caagatcttg
4441 aacctggtgc tccatccatg ggagctaaaa gcctttgcat ccccttcaaa ccactctgtg
4501 aactgcagcc tggagccaaa tgtgtctgtg gcaagaaccc tgccaagtac tacaccttat
4561 ttggtcgcag ctactgaggg atgaacgaaa gccccctctt c
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