LOCUS BC136348 3191 bp mRNA linear HUM 12-MAY-2008
DEFINITION Homo sapiens TAF5 RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 100kDa, mRNA (cDNA clone MGC:167958
IMAGE:9020335), complete cds.
ACCESSION BC136348
VERSION BC136348.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3191)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3191)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: LLDM Plate: 670 Row: e Column: 5.
FEATURES Location/Qualifiers
source 1..3191
/db_xref="H-InvDB:HIT000500373"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:167958 IMAGE:9020335"
/tissue_type="Testis, PCR rescued clones"
/clone_lib="NIH_MGC_374"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: TGGGCAAC"
gene 1..3191
/gene="TAF5"
/gene_synonym="TAFII100"
/db_xref="GeneID:6877"
/db_xref="HGNC:HGNC:11539"
/db_xref="MIM:601787"
CDS 19..2421
/gene="TAF5"
/gene_synonym="TAFII100"
/codon_start=1
/product="TAF5 RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 100kDa"
/protein_id="AAI36349.1"
/db_xref="GeneID:6877"
/db_xref="HGNC:HGNC:11539"
/db_xref="MIM:601787"
/translation="MAALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGP
NGGGGNVAASSSTGGDGGTPKPTVAVSAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQ
FLRQSKLREAEEALRREAGLLEEAVAGAGAPGEVDSAGAEVTSALLSRVTASAPGPAA
PDPPGTGASGATVVSGSASGPAAPGKVGSVAVEDQPDVSAVLSAYNQQGDPTMYEEYY
SGLKHFIECSLDCHRAELSQLFYPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQ
DDLRVLSSLTKKEHMKGNETMLDFRTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQ
EHLYIDIFDGMPRSKQQIDAMVGSLAGEAKREANKSKVFFGLLKEPEIEVPLDDEDEE
GENEEGKPKKKKPKKDSIGSKSKKQDPNAPPQNRIPLPELKDSDKLDKIMNMKETTKR
VRLGPDCLPSICFYTFLNAYQGLTAVDVTDDSSLIAGGFADSTVRVWSVTPKKLRSVK
QASDLSLIDKESDDVLERIMDEKTASELKILYGHSGPVYGASFSPDRNYLLSSSEDGT
VRLWSLQTFTCLVGYKGHNYPVWDTQFSPYGYYFVSGGHDRVARLWATDHYQPLRIFA
GHLADVNCTRFHPNSNYVATGSADRTVRLWDVLNGNCVRIFTGHKGPIHSLTFSPNGR
FLATGATDGRVLLWDIGHGLMVGELKGHTDTVCSLRFSRDGEILASGSMDNTVRLWDA
IKAFEDLETDDFTTATGHINLPENSQELLLGTYMTKSTPVVHLHFTRRNLVLAAGAYS
PQ"
BASE COUNT 888 a 684 c 796 g 823 t
ORIGIN
1 aggtggctca gccgcaagat ggcggcgctg gcggaggagc agacggaggt ggcggtcaag
61 ctagagcctg agggaccgcc aacgctgcta cctccgcagg cgggggacgg cgcaggcgag
121 ggtagcggcg gcactaccaa caacggcccc aacggcggcg gcgggaacgt tgcggcgtcg
181 tcgtccactg gcggggatgg cgggaccccc aagcccacgg tggctgtctc cgccgctgcc
241 ccggcggggg cggccccggt gcccgccgct gctccggacg ccggcgctcc gcatgaccga
301 cagactctac tggccgtgct gcagttccta cggcagagca aactccgcga ggccgaagag
361 gcgctgcgcc gtgaggccgg gctgctggag gaggcagtgg cgggcgccgg agccccggga
421 gaggtggaca gcgccggcgc tgaggtgacc agcgcgcttc tcagccgggt gaccgcctcg
481 gcccctggcc ctgcggcccc cgaccctccg ggcactggcg cttcgggggc cacggtcgtc
541 tcaggttcag cctcaggtcc tgcggctccg ggtaaagttg gaagtgttgc tgtggaagac
601 cagccagatg tcagtgccgt gttgtcagcc tacaaccaac aaggagatcc cacaatgtat
661 gaagaatact atagtggact gaaacacttc attgaatgtt ccctggactg ccatcgggca
721 gagttgtccc aactttttta tcctctgttt gtgcacatgt acttggagct agtctacaat
781 caacatgaga atgaagcaaa gtcattcttt gagaagttcc atggagatca ggaatgttat
841 taccaggatg acctacgagt attatctagt cttaccaaaa aggaacacat gaaagggaat
901 gagaccatgt tggattttcg aacaagtaaa tttgttctgc gtatttcccg tgactcgtac
961 caactcttga agaggcatct tcaggagaaa cagaacaatc agatatggaa catagttcag
1021 gagcacctct acattgacat ctttgatggg atgccgcgta gtaagcaaca gatagatgcg
1081 atggtgggaa gtttggcagg agaggctaaa cgagaggcaa acaaatcaaa ggtatttttt
1141 ggtttattaa aagaaccaga aattgaggta cctttggatg acgaggatga agagggagaa
1201 aatgaagaag gaaaacctaa aaagaagaag cctaaaaaag atagtattgg atccaaaagc
1261 aaaaaacaag atcccaatgc tccacctcag aacagaatcc ctcttcctga gttgaaagat
1321 tcagataagt tggataagat aatgaatatg aaagaaacca ccaaacgagt gcgccttggg
1381 ccggactgct taccctccat ttgtttctat acatttctca atgcttacca gggtctcact
1441 gcagtggatg tcactgatga ttctagtctg attgctggag gttttgcaga ttcaactgtc
1501 agagtgtggt cggtaacacc caaaaagctt cgtagtgtca aacaagcatc agatcttagt
1561 cttatagaca aagaatcaga tgatgtctta gaaagaatca tggatgagaa aacagcaagt
1621 gagttgaaga ttttgtatgg tcacagtggg cctgtctacg gagccagctt cagtccggat
1681 aggaactatc tgctttcctc ttcagaggac ggaactgtta gattgtggag ccttcaaaca
1741 tttacttgtt tggtgggata taaaggacac aactatccag tatgggacac acaattttct
1801 ccatatggat attattttgt gtcagggggc catgaccgag tagctcggct ctgggctaca
1861 gaccactatc agcctttaag aatatttgcc ggccatcttg ctgatgtgaa ttgtaccaga
1921 ttccatccaa attctaatta tgttgctacg ggctctgcag acagaactgt gcggctctgg
1981 gacgtcctga atggtaactg tgtaaggatc ttcactggac acaagggacc aattcattcc
2041 ttgacatttt ctcccaatgg gagattcctg gctacaggag caacagatgg cagagtgctt
2101 ctttgggata ttggacatgg tttgatggtt ggagaattaa aaggccacac tgatacagtc
2161 tgttcactta ggtttagtag agatggtgaa attttggcat caggttcaat ggataataca
2221 gttcgattat gggatgctat caaagccttt gaagatttag agaccgatga ctttactaca
2281 gccactgggc atataaattt acctgagaat tcacaggagt tattgttggg aacatatatg
2341 accaaatcaa caccagttgt acaccttcat tttactcgaa gaaacctggt tctagctgca
2401 ggagcttata gtccacaata aaccatcggt attaaagacc ttttggaagc tactgttttt
2461 aaaaagggag actaaaagca aatacctcag tgattaatat ttaagctaca gagaatgttt
2521 ttgtctatat ggatctggaa gtatgctgct tggaaaaatc tgaacaggac agttccacgt
2581 ttctatagca accacatttg actaatttcc gttagttgaa taagaggtat tatgatcatg
2641 gaggggacat ttatggtgct ttggattgtg tggaaactat gcattttctg ttcaaatgct
2701 attttaattt attacattta gaaaaaaagt tgatttcaat aattcatcct gcttcaagat
2761 tcaaattcag aaatatacta tcatcttgaa ttttagctga agaatcctat gagcatgtat
2821 gtttctgctg taaaaacgta gttactgtat ggcactcaaa aactatgtta aatgatccac
2881 taactttttt tttcttggcc catgattaat ggaatgtatg taactaggta gggttccttt
2941 cttagatcta gaggaagtac agccacccac tgacatctga atttatatac ctgttgagtt
3001 ttgagtgcac ccaaacactc gataaaccag gtgaagaaat ttagcttcca tgttctactt
3061 cagctaaaac agctacatac aacctagtac acttgaagtc agacagacat ttcagttgct
3121 tacctccagt actgagcctt gctttgggaa actaaaagat ttagaccaag tcactgccag
3181 tttttgcctt t
//