LOCUS BC133655 813 bp mRNA linear HUM 06-MAR-2007
DEFINITION Homo sapiens BRF1 homolog, subunit of RNA polymerase III
transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA
clone IMAGE:40003194), complete cds.
ACCESSION BC133655
VERSION BC133655.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 813)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 813)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-MAR-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCG Plate: 18 Row: g Column: 12
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..813
/db_xref="H-InvDB:HIT000391104"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:40003194"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_283"
/note="Vector: pCR-Blunt II-TOPO with reversed insert;
Clone identification sequence tag: AGTATTCT sequenced from
the forward primer"
gene 1..813
/gene="BRF1"
/gene_synonym="BRF"
/gene_synonym="hBRF"
/gene_synonym="TAFIII90"
/gene_synonym="TF3B90"
/gene_synonym="TFIIIB90"
/db_xref="GeneID:2972"
/db_xref="HGNC:HGNC:11551"
/db_xref="MIM:604902"
CDS 192..746
/gene="BRF1"
/gene_synonym="BRF"
/gene_synonym="hBRF"
/gene_synonym="TAFIII90"
/gene_synonym="TF3B90"
/gene_synonym="TFIIIB90"
/codon_start=1
/product="BRF1 protein"
/protein_id="AAI33656.1"
/db_xref="GeneID:2972"
/db_xref="HGNC:HGNC:11551"
/db_xref="MIM:604902"
/translation="MTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKE
VISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQL
EQVLSKKLEEVEGEISSYQDAIEIELENSPPSRREGRRRPESLPRPLRGKAPVPLVGG
LSSGMAAPDVAEGASPCVLRRGWP"
BASE COUNT 183 a 227 c 253 g 150 t
ORIGIN
1 ccgccaagga cacacccacc gaggccacag ctgcgatttc atagtgtggg accccactgg
61 caaagacacg ctggctgctg tggccgtggt ttctgtccca gccgtctgtt tccatagaaa
121 aagacccgtg cctgtatatt ccacgctttg cgcacctgct ggaattcggg gagaagaacc
181 acgaggtgtc catgactgcc ctgaggctcc tacagaggat gaagcgggac tggatgcaca
241 caggccggcg cccctcgggc ctctgcggag cagcgctcct ggttgcagcc agaatgcatg
301 acttcaggag gactgtgaag gaggtcatca gtgtggtcaa agtgtgtgag tccacgctgc
361 ggaagaggct cacggaattt gaagacaccc ccaccagtca gttgaccatt gatgagttca
421 tgaagatcga cctggaggag gagtgcgacc ccccctcgta cacagctggg cagaggaagc
481 tgcggatgaa gcagcttgaa caagtcctgt caaaaaaact ggaggaggtt gaaggtgaaa
541 tatccagtta ccaggatgca attgagattg aactagaaaa cagcccccca tcgaggaggg
601 agggcagacg gaggcccgag agcctcccca ggcctcttcg tgggaaggcc ccagtaccac
661 tcgtaggagg tctcagctct ggcatggctg ccccggatgt ggccgagggg gcttcaccct
721 gtgtccttag gagggggtgg ccttgaggca gagccgtgcc tcactgaccc ccaggggcct
781 catcctcccc atggaatggg ctgtatgtcc tgc
//