LOCUS BC132997 1736 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens amylase, alpha 1A (salivary), mRNA (cDNA clone
MGC:164628 IMAGE:40147019), complete cds.
ACCESSION BC132997
VERSION BC132997.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1736)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1736)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 9 Row: N Column: 19.
FEATURES Location/Qualifiers
source 1..1736
/db_xref="H-InvDB:HIT000391048_06"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:164628 IMAGE:40147019"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_362"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CTTTGCCC"
gene 1..1736
/gene="AMY1A"
/gene_synonym="AMY1B"
/db_xref="GeneID:276"
/db_xref="HGNC:HGNC:474"
/db_xref="MIM:104700"
CDS 171..1706
/gene="AMY1A"
/gene_synonym="AMY1B"
/codon_start=1
/product="amylase, alpha 1A (salivary)"
/protein_id="AAI32998.1"
/db_xref="GeneID:276"
/db_xref="HGNC:HGNC:474"
/db_xref="MIM:104700"
/translation="MKLFWLLFTIGFCWAQYSSNTQQGRTSIVHLFEWRWVDIALECE
RYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
NVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
GDIENYNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASKHMW
PGDIKAILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK
LGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDAR
LYKMAVGFMLAHPYGFTRVMSSYRWPRYFENGKDVNDWVGPPNDNGVTKEVTINPDTT
CGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWT
FSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAE
SKL"
BASE COUNT 503 a 305 c 419 g 509 t
ORIGIN
1 tcggtctgtc agggttggaa agtccaagcc ataggaccca gtttcctttc ttagcttacg
61 ttatctacca gagcaccgtg ggctgttact tgccttgagt tggaagcggt tcgcatttat
121 accggaatat aaatagtttc tggaaaggac actgacaact tcaaagcaaa atgaagctct
181 tttggttgct tttcaccatt gggttctgct gggctcagta ttcctcaaat acacaacaag
241 gacgaacatc tattgttcat ctgtttgaat ggcgatgggt tgatattgct cttgaatgtg
301 agcgatattt agctcccaag ggatttggag gggttcaggt ctctccacca aatgaaaatg
361 ttgccattca caaccctttc agaccttggt gggaaagata ccaaccagtt agctataaat
421 tatgcacaag atctggaaat gaagatgaat ttagaaacat ggtgactaga tgcaacaatg
481 ttggggttcg tatttatgtg gatgctgtaa ttaatcatat gtgtggtaat gctgtgagtg
541 caggaacaag cagtacctgt ggaagttact tcaaccctgg aagtagggac tttccagcag
601 tcccatattc tggatgggat tttaatgatg gtaaatgtaa aactggaagt ggagatatcg
661 agaactataa tgatgctact caggtcagag attgtcgtct gtctggtctt ctcgatcttg
721 cactggggaa ggattatgtg cgttctaaga ttgccgaata tatgaaccat ctcattgaca
781 ttggtgttgc agggttcaga attgatgctt ccaagcacat gtggcctgga gacataaagg
841 caattttgga caaactgcat aatctaaaca gtaactggtt cccggaaggt agtaaacctt
901 tcatttacca ggaggtaatt gatctgggtg gtgagccaat taaaagcagt gactactttg
961 gtaatggccg ggtgacagaa ttcaagtatg gtgcaaaact cggcacagtt attcgcaagt
1021 ggaatggaga gaagatgtct tacttaaaga actggggaga aggttggggt ttcatgcctt
1081 ctgacagagc gcttgtcttt gtggataacc atgacaatca acgaggacat ggcgctggag
1141 gagcctctat acttaccttc tgggatgcta ggctgtacaa aatggcagtt ggatttatgc
1201 ttgctcatcc ttatggattt acacgagtaa tgtcaagcta ccgttggcca agatattttg
1261 aaaatggaaa agatgttaat gattgggttg ggccaccaaa tgataatgga gtaactaaag
1321 aagttactat taatccagac actacttgtg gcaatgactg ggtctgtgaa catcgatggc
1381 gccaaataag gaacatggtt aatttccgca atgtagtgga tggccagcct tttacaaact
1441 ggtatgataa tgggagcaac caagtggctt ttgggagagg aaacagagga ttcattgttt
1501 tcaacaatga tgactggaca ttttctttaa ctttgcaaac tggtcttcct gctggcacat
1561 actgtgatgt catttctgga gataaaatta atggcaactg cacaggcatt aaaatctacg
1621 tttctgatga tggcaaagct catttttcta ttagtaactc tgctgaagat ccatttattg
1681 caattcatgc tgaatctaaa ttgtaaaatt taaaattaaa tgcaaatccg caaagc
//