LOCUS BC132995 1735 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens amylase, alpha 1A (salivary), mRNA (cDNA clone
MGC:164626 IMAGE:40147017), complete cds.
ACCESSION BC132995
VERSION BC132995.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1735)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1735)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 9 Row: N Column: 17.
FEATURES Location/Qualifiers
source 1..1735
/db_xref="H-InvDB:HIT000391046_06"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:164626 IMAGE:40147017"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_362"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CCCCGCCA"
gene 1..1735
/gene="AMY1A"
/gene_synonym="AMY1B"
/db_xref="GeneID:276"
/db_xref="HGNC:HGNC:474"
/db_xref="MIM:104700"
CDS 170..1705
/gene="AMY1A"
/gene_synonym="AMY1B"
/codon_start=1
/product="amylase, alpha 1A (salivary)"
/protein_id="AAI32996.1"
/db_xref="GeneID:276"
/db_xref="HGNC:HGNC:474"
/db_xref="MIM:104700"
/translation="MKLFWLLFTIGFCWAQYSSNTQQGRTSIVHLFEWRWVDIALECE
RYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
NVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
GDIENYNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASKHMW
PGDIKAILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK
LGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDAR
LYKMAVGFMLAHPYGFTRVMSSYRWPRYFENGKDVNDWVGPPNDNGVTKEVTINPDTT
CGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWT
FSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAE
SKL"
BASE COUNT 503 a 305 c 419 g 508 t
ORIGIN
1 cggtctgtca gggttggaaa gtccaagcca taggacccag tttcctttct tagcttacgt
61 tatctaccag agcaccgtgg gctgttactt gccttgagtt ggaagcggtt cgcatttata
121 ccggaatata aatagtttct ggaaaggaca ctgacaactt caaagcaaaa tgaagctctt
181 ttggttgctt ttcaccattg ggttctgctg ggctcagtat tcctcaaata cacaacaagg
241 acgaacatct attgttcatc tgtttgaatg gcgatgggtt gatattgctc ttgaatgtga
301 gcgatattta gctcccaagg gatttggagg ggttcaggtc tctccaccaa atgaaaatgt
361 tgccattcac aaccctttca gaccttggtg ggaaagatac caaccagtta gctataaatt
421 atgcacaaga tctggaaatg aagatgaatt tagaaacatg gtgactagat gcaacaatgt
481 tggggttcgt atttatgtgg atgctgtaat taatcatatg tgtggtaatg ctgtgagtgc
541 aggaacaagc agtacctgtg gaagttactt caaccctgga agtagggact ttccagcagt
601 cccatattct ggatgggatt ttaatgatgg taaatgtaaa actggaagtg gagatatcga
661 gaactataat gatgctactc aggtcagaga ttgtcgtctg tctggtcttc tcgatcttgc
721 actggggaag gattatgtgc gttctaagat tgccgaatat atgaaccatc tcattgacat
781 tggtgttgca gggttcagaa ttgatgcttc caagcacatg tggcctggag acataaaggc
841 aattttggac aaactgcata atctaaacag taactggttc ccggaaggta gtaaaccttt
901 catttaccag gaggtaattg atctgggtgg tgagccaatt aaaagcagtg actactttgg
961 taatggccgg gtgacagaat tcaagtatgg tgcaaaactc ggcacagtta ttcgcaagtg
1021 gaatggagag aagatgtctt acttaaagaa ctggggagaa ggttggggtt tcatgccttc
1081 tgacagagcg cttgtctttg tggataacca tgacaatcaa cgaggacatg gcgctggagg
1141 agcctctata cttaccttct gggatgctag gctgtacaaa atggcagttg gatttatgct
1201 tgctcatcct tatggattta cacgagtaat gtcaagctac cgttggccaa gatattttga
1261 aaatggaaaa gatgttaatg attgggttgg gccaccaaat gataatggag taactaaaga
1321 agttactatt aatccagaca ctacttgtgg caatgactgg gtctgtgaac atcgatggcg
1381 ccaaataagg aacatggtta atttccgcaa tgtagtggat ggccagcctt ttacaaactg
1441 gtatgataat gggagcaacc aagtggcttt tgggagagga aacagaggat tcattgtttt
1501 caacaatgat gactggacat tttctttaac tttgcaaact ggtcttcctg ctggcacata
1561 ctgtgatgtc atttctggag ataaaattaa tggcaactgc acaggcatta aaatctacgt
1621 ttctgatgat ggcaaagctc atttttctat tagtaactct gctgaagatc catttattgc
1681 aattcatgct gaatctaaat tgtaaaattt aaaattaaat gcaaatccgc aaagc
//