LOCUS BC132674 6626 bp mRNA linear HUM 06-DEC-2007
DEFINITION Homo sapiens mediator complex subunit 14, mRNA (cDNA clone
MGC:164305 IMAGE:40146696), complete cds.
ACCESSION BC132674
VERSION BC132674.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6626)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 6626)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 9 Row: A Column: 8.
FEATURES Location/Qualifiers
source 1..6626
/db_xref="H-InvDB:HIT000390729"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:164305 IMAGE:40146696"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_364"
/lab_host="DH10B"
/note="Vector: pCR-XL-TOPO with reversed insert; Clone
identification sequence tag: GCCTGTCC"
gene 1..6626
/gene="MED14"
/gene_synonym="CRSP150"
/gene_synonym="CSRP"
/gene_synonym="DRIP150"
/gene_synonym="EXLM1"
/gene_synonym="RGR1"
/gene_synonym="TRAP170"
/db_xref="GeneID:9282"
/db_xref="HGNC:HGNC:2370"
/db_xref="MIM:300182"
CDS 110..4474
/gene="MED14"
/gene_synonym="CRSP150"
/gene_synonym="CSRP"
/gene_synonym="DRIP150"
/gene_synonym="EXLM1"
/gene_synonym="RGR1"
/gene_synonym="TRAP170"
/codon_start=1
/product="mediator complex subunit 14"
/protein_id="AAI32675.1"
/db_xref="GeneID:9282"
/db_xref="HGNC:HGNC:2370"
/db_xref="MIM:300182"
/translation="MAPVQLENHQLVPPGGGGGGSGGPPSAPAPPPPGAAVAAAAAAA
ASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLAL
VKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDV
LTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVA
NGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQ
LVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKC
LSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKI
DHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSE
CLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQS
IKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSY
KYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVE
SKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAI
RLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQG
PSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFS
EVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQ
EMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHI
RLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQD
ARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHS
TVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPH
GTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPR
AGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLC
SPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQ
LKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIM
KLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQ
EPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIF
AAVRDLMANLTLPPGGRP"
BASE COUNT 1832 a 1507 c 1316 g 1971 t
ORIGIN
1 gtaggcggtg gcgactctgc ccgctctcgt ttcggcgcgg tgaccgagcg cccgggaggc
61 tcgaggaccg catcgtgtgc cgttgcgcca agcccggtcc tgcgccgcca tggccccagt
121 gcagctggag aaccaccagc tggtcccgcc cggaggcggc ggcgggggca gcggcggacc
181 cccgtcagcc ccagcccctc ctcccccggg agccgccgtg gcggcggccg ctgcagctgc
241 ggctagcccg ggctaccggc tgagcaccct cattgaattt ctgctgcacc gggcctactc
301 ggagcttatg gtgttgacgg acctactgcc aaggaaatct gatgtggaaa ggaaaataga
361 aatagtgcag tttgctagcc ggacacgcca actcttcgtt cgattattag ctttagtgaa
421 atgggctaat aatgctggca aagtggaaaa atgtgcgatg atttcaagct ttttagatca
481 gcaagccatc ctgtttgtgg acactgctga tcgcctggcc tcgttagcta gagatgctct
541 ggtccatgca cgcctgccta gttttgccat cccatatgcc attgatgtac taactactgg
601 atcttaccca cggctgccaa cctgcattag ggacaaaatt attcctccag acccaattac
661 caaaattgaa aaacaagcca cactccatca gctgaatcag attcttagac atcggcttgt
721 aaccacagat cttcctcctc agttagcaaa tcttacagtt gcaaatggcc gggtgaagtt
781 tcgtgttgaa ggagaatttg aagccacctt gactgtgatg ggagatgacc ctgatgttcc
841 atggcgtctt ctcaagctag aaattctagt tgaggataag gaaacaggag atgggcgagc
901 tttggttcat agcatgcaaa tcagcttcat ccatcaactg gtgcagtcta ggctctttgc
961 tgatgagaaa cctcttcagg atatgtacaa ctgcctacat tctttctgtt tatcacttca
1021 gttagaagtg ttacattccc aaactctaat gttaatccga gaacggtggg gagaccttgt
1081 gcaggtggaa aggtatcatg ctggaaagtg cctctccctt tcagtttgga atcaacaggt
1141 tcttgggaga aaaactggaa cagcatctgt tcacaaagtt acaattaaaa ttgatgagaa
1201 tgatgtctcc aagcctttac agatttttca cgatcctcct ttgccagctt ctgattccaa
1261 attagtagaa agagccatga agatcgacca cttatcaata gaaaaactcc tgattgacag
1321 tgtccatgca agagctcatc agaagctcca agaactgaag gccattctta gaggcttcaa
1381 tgccaatgaa aactcttcca tagagactgc actcccagct cttgttgttc ccatcttgga
1441 gccctgtggt aattcagagt gtctgcacat ttttgtagat ttacattctg gaatgtttca
1501 attgatgctt tatggacttg accaggccac tctggatgac atggagaagt ctgtgaatga
1561 tgatatgaaa cgaatcatac cctggattca gcaacttaag ttttggcttg gacaacagcg
1621 ttgcaagcag tctataaaac atctgcctac gataagcagt gaaacattgc agctttccaa
1681 ttactcaact catcctattg gaaacctttc taagaataaa ctgttcatta aacttacccg
1741 ccttccacaa tactacattg ttgtggagat gttggaggtt cccaataaac ccacacaact
1801 gtcgtacaag tactacttta tgtctgtgaa tgctgcagat cgtgaagaca gccctgcaat
1861 ggcattgctg ctgcagcagt tcaaggaaaa cattcaggac ttggtttttc gtacaaaaac
1921 cgggaaacag accagaacca atgccaagcg caagttgtct gatgatccat gtccagtaga
1981 atccaagaaa acaaaacgag caggagaaat gtgtgccttc aataaagttt tagcccactt
2041 cgtcgctatg tgtgatacaa atatgccatt tgtaggactt cggttggagt tgtccaatct
2101 ggagattcca catcaaggag tgcaagtgga aggtgatggc ttcagccatg caattcgctt
2161 attaaaaatt cctccctgta agggtataac tgaggaaacc caaaaggctc tggaccgctc
2221 tcttcttgat tgcactttcc gattacaagg tagaaataac cgcacttggg tagcagagtt
2281 agtgtttgca aattgtccac ttaatggcac ttctactagg gagcaaggac catcccggca
2341 cgtttacctg acatatgaaa atctgttgtc tgagcctgtt ggtggtagaa aggtggttga
2401 aatgtttctt aatgactgga atagcattgc acgattatat gagtgtgtgt tggaatttgc
2461 acgttctcta ccagacatac ctgctcatct aaatattttc tcagaagttc gtgtttataa
2521 ttaccgaaaa cttatcttgt gttatggaac caccaaggga agctcaatta gtatccaatg
2581 gaattcgatc catcaaaaat tccacatttc tttgggaact gttggcccaa actcaggttg
2641 cagtaactgt cacaatacca ttctccatca gcttcaagaa atgttcaaca aaacaccaaa
2701 tgtggttcag ttattacagg tactgtttga tactcaggct ccattaaatg ccatcaacaa
2761 actccccact gtgccgatgt tgggcttgac ccagagaacc aatactgcct accagtgctt
2821 ctccattctg ccacagtcgt ccacccacat cagactggcc ttcaggaaca tgtattgcat
2881 tgatatatac tgccggagtc gaggtgttgt ggcaatacgg gatggtgcct atagtctttt
2941 tgataacagc aaactagttg aaggtttcta tcctgcacca ggactaaaga cgttcctgaa
3001 tatgtttgtt gacagcaatc aggatgctcg aagaaggtct gtaaatgagg acgataatcc
3061 cccttctcct ataggaggag atatgatgga ttctttaata tcgcagctcc agccaccacc
3121 ccagcaacag ccatttccaa agcagccagg aacatcaggt gcttatcctc ttacttcacc
3181 ccctacatct tatcatagca cagtcaatca gtctccctca atgatgcaca cacagtctcc
3241 aggaaatctg catgctgcca gctcccccag tggggctttg agagccccat caccagcgtc
3301 atttgttcca actcctcccc catcctcgca tggaatctca ataggaccag gggccagttt
3361 tgctagtcca catggaactc ttgaccctag ttccccatac actatggtgt caccaagtgg
3421 acgagcaggg aactggccag gatctcctca agtgtctggc ccctcaccag cagcacgcat
3481 gcctggaatg tcaccagcca acccctcact acattctccg gttccagatg cttctcattc
3541 ccctcgagct ggaacaagtt ctcaaacaat gccaacaaac atgcctccac ctcgtaaact
3601 acctcagcgc tcttgggcgg catccatacc taccatcctc actcacagtg ccttgaacat
3661 tttactgctg ccctctccaa ctccaggcct tgtgcccggc ctggcaggta gttacctttg
3721 ttctccactt gagagattcc ttggatcagt catcatgaga cgacatcttc aaagaattat
3781 tcaacaagaa acgctgcagc tgataaattc taatgaaccc ggagtgatca tgtttaagac
3841 tgatgcactg aaatgcagag tagctcttag tcccaaaacc aaccaaacgc ttcagctaaa
3901 agtgacacct gaaaatgcag gacagtggaa acctgatgaa cttcaagttt tggagaaatt
3961 ctttgaaaca agagttgcag gaccaccatt taaagctaat acgttaatag ccttcaccaa
4021 gctattagga gctcctacac acatcctcag ggattgtgtg cacattatga agctggagct
4081 gttccctgac caagcaacac agctaaaatg gaatgttcag ttttgcctga cgatccctcc
4141 cagcgcgccg ccgattgcac ctcctgggac gcctgctgtg gtgctgaaat ccaaaatgct
4201 attttttctt caactaactc agaaaacatc ggtccctccc caagaacctg ttagtattat
4261 agttccaatc atttatgaca tggcttcagg tacaacccag caggcagaca ttcccagaca
4321 gcagaactct tctgttgctg ctcccatgat ggtcagcaac attctgaaga ggtttgcaga
4381 gatgaatcca ccacgacaag gtgaatgcac aatatttgca gctgttcgtg atttaatggc
4441 taatcttaca ctgccccctg gtgggcgtcc atagacacta ttgtttttaa accaggaagg
4501 ctgacagatg agacaacaaa aaaaatctga attcagcctt cagtttaaaa aaggaaaagg
4561 actttttttt taaactttaa gagctaaata ttctaaactg gcaaaaattt tcagggtgca
4621 cttctttcat caaaatgatc atccatcttt tgtataatga acttgtatag tgtgtttaaa
4681 tgggacacat ttcaaaggaa ataaatcagt gtccgtttgc cagtaataaa tttaatattc
4741 tgttttatac tataaagtta tgaaaatgcc aaacttgttt atttaattgt tttcttatgt
4801 tttgggtgta atagtccttt tttggttttt gttttgtttt ttaaggcagg tctgtcattt
4861 ttgaatactg taaaactgtg ataaacttta tattgaagct gtattttaat atgaccgctt
4921 tgaatcctta catgaaatgt tctgggagtt gttataaaca caccccaagg aagcaatatt
4981 taataaaact aggtcaaaaa caaaacaaaa cagtgacact cacttgtgtg tatataaact
5041 ctagagacat cttaggccct cccttcatct ttaacctggt ggatggagcc agttattaat
5101 ttataataat acatgtctga aatttgacca aaaacatctt ctttatgtca aggttaatat
5161 attttcttca gacattccta acttgactag tggttaacat ttagcacctt ggttgtcttt
5221 caatatatag gatttgggtg aaaaataagg aacttagtct ttgagaaggg acagtatgtg
5281 gttcgtattg atcaaggatc ccaaataatg caatcattct catctgttga ggatgtggga
5341 acttttctgt caggaaaatt tagtttacag aatacttctg catacaacat taattaggaa
5401 acaaagaact ttaatgctgt acctctgcat gaagtttctg tgagctttct ttatcttgct
5461 taacttatcc tcctttcctt ccccaccaaa attgggattg ttctaaacaa tttcaattta
5521 attcattgtc aacaaaacga tagaatgttt tgcttggaag aaacctggtc cattcccctc
5581 attgtgaaga atcccgaatc ctgggcctgt ccagggtcaa atggagtttt tggtattaat
5641 acatctctgc agggtttctt tttggagcac tgtgctgtct ttcctagtca gtcagggact
5701 tttttaagct tcaaagaaca tggtctgatg caaaggtctt ctaccgactt catggtattt
5761 aggaaaaatt tttaagtgat aataccatgc cgagttacct ggtcactgta atactggttc
5821 tccattaaaa tgtagatgct ttgtctaatt ccacttcaag atgatttaat tgagcttcta
5881 taactatcaa ctatcaaatt ctagtttcaa gtatctcact gtacctatat catatctgtt
5941 ctcaactttg tttgattctc cactcatttt caatcagagt tgaagccttc tttgcctttc
6001 ttctttgtct tatttctgcc ttcatctccc acttctcacc caactctgcc actgttcagg
6061 agcagttgac taaactttta tccttccttt aacttaatct ttttatcctt ctgtttttag
6121 ttcttacagt ggtaatcaca tcagtttggc aatctaagat gtctttatca agggtcagcc
6181 cttttctatt tggagctgaa tcttttcttt catgtagaaa tctacatgta tatacaagaa
6241 atgtattttt atctgcagga taatattgtt tcaaataatt ttattaagag ttaacttttt
6301 aggtattttc ccttttaatg tggttttgaa atgttaattg catttttaca catgatgcat
6361 tcagtggaca ctcttttttc ctaattataa ctcatctctg aatgcattgt gccaatcaag
6421 agtaagatag gattcagtgc gcaaaatttg cttttgatgc ttattaagag taaacccatg
6481 aatttgaatt tttatattat gcttggtact tggaattgat atatacctta ttaaagcttg
6541 aaaacattta gcatttgaat agtaagtttc atcacaaatc atgtcttttg cctcttttgt
6601 tacccaggtt cgatcaaggg gactgg
//