LOCUS BC130484 2469 bp mRNA linear HUM 04-JAN-2007
DEFINITION Homo sapiens guanylate cyclase 1, soluble, alpha 2, mRNA (cDNA
clone MGC:163356 IMAGE:40146515), complete cds.
ACCESSION BC130484
VERSION BC130484.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2469)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2469)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (04-JAN-2007) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 8 Row: I Column: 19.
FEATURES Location/Qualifiers
source 1..2469
/db_xref="H-InvDB:HIT000390274"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:163356 IMAGE:40146515"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_362"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CCCCGCAT"
gene 1..2469
/gene="GUCY1A2"
/gene_synonym="GC-SA2"
/db_xref="GeneID:2977"
/db_xref="HGNC:HGNC:4684"
/db_xref="MIM:601244"
CDS 97..2295
/gene="GUCY1A2"
/gene_synonym="GC-SA2"
/codon_start=1
/product="guanylate cyclase 1, soluble, alpha 2"
/protein_id="AAI30485.1"
/db_xref="GeneID:2977"
/db_xref="HGNC:HGNC:4684"
/db_xref="MIM:601244"
/translation="MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPG
PLEPSPAAAAAAAAPAPTPAASAAAAAATAGARRVQRRRRVNLDSLGESISRLTAPSP
QTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYADHSNKEEIEDVSGILQCTAN
ILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHIRTSFGKQA
TLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKL
CSDVSNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFD
PSMSVLQLGEGLRKQLRCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKP
EASGSENKDKVMEVKGQMIHVPESNSILFLGSPCVDKLDELMGRGLHLSDIPIHDATR
DVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQ
GQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVE
TIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQMRIGIHSGSVLA
GVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREESFTFIPRSREEL
PDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL"
BASE COUNT 644 a 611 c 614 g 600 t
ORIGIN
1 cttaacgttg tcgcttgccg gtcccgccac cgccgcctcc gccgccgctc gcgtcctcgc
61 cgccaccgcc tcggccgctg cagctccgcc ggcagcatgt ctcgaaggaa gatttcgtcc
121 gagtccttca gctccctggg ctccgactac ctggagacca gcccggagga ggagggggag
181 tgccccctgt ctaggctctg ctggaatggc agccggagcc cgcccgggcc gctggagccc
241 agcccggccg cagctgccgc tgccgccgct ccggccccga ccccggctgc ttctgccgcc
301 gccgccgctg ccactgccgg ggccaggagg gtgcagcgcc ggaggcgggt caacctggac
361 tcgctgggcg agagcatcag ccgcctgacg gcgccctcgc ctcagacgat acagcagact
421 ctcaagagga cactgcagta ttatgaacat caagttattg gttacaggga tgcagaaaag
481 aatttccaca atatctctaa cagatgctcc tatgcagacc actccaataa agaagaaatt
541 gaagatgtct caggaattct tcagtgtact gctaatatac tcggtttgaa gtttgaggaa
601 attcaaaaaa gatttggtga agagttcttt aatatatgct ttcatgagaa tgagagagtc
661 cttcgagctg taggtggcac tttgcaggac ttttttaacg gctttgatgc tttgttggaa
721 cacattagaa cttcttttgg aaaacaggcc actctggagt caccatcttt cctatgcaaa
781 gagctccctg aaggtactct catgctccac tacttccacc ctcaccatat tgtggggttt
841 gcaatgctgg ggatgattaa ggctgcagga aagaagatct atcggctgga tgtggaagtg
901 gaacaggttg caaatgagaa gctatgctct gatgtttcaa acccaggcaa ttgtagctgt
961 cttactttcc ttatcaaaga atgtgaaaat actaatatca tgaagaacct tccacaggga
1021 acctcccaag ttcctgcgga cctcagaatt agcatcaaca ccttctgtag agccttccct
1081 ttccacttga tgtttgatcc cagcatgtca gtccttcagt tgggggaagg tctaaggaag
1141 cagcttcgat gtgacactca caaagtgctc aagtttgagg actgcttcga gattgtatct
1201 ccaaaggtta atgccacctt tgaaagggtc ctgctgcgac tgtctacccc gtttgtgatt
1261 agaaccaagc ctgaggcttc tggctctgaa aataaagaca aggtgatgga agtcaaagga
1321 caaatgatcc atgttccaga atcaaattcc attttatttt tgggctctcc atgtgtggac
1381 aagttggatg aactcatggg ccgagggcta catctctcag acatccctat ccatgatgcc
1441 acccgagatg tcattttggt tggtgagcag gcaaaggccc aagatgggtt gaagaaaagg
1501 atggataaat taaaggcaac tttagaaaga actcaccagg ccctggaaga agagaaaaag
1561 aagacagtgg atcttctata ttctattttc cctggtgatg tagcccagca attatggcaa
1621 gggcagcaag tacaggccag aaagtttgat gatgtcacca tgctcttttc agacattgtt
1681 ggcttcacag ccatatgtgc ccagtgtact cccatgcaag taatcagcat gctgaatgaa
1741 ctgtacacca gatttgacca ccagtgtgga tttttggata tttataaggt ggaaacaata
1801 ggtgatgcct actgtgttgc agcagggctc cacagaaaaa gcctctgcca tgctaaaccc
1861 attgctctga tggccttgaa gatgatggaa ctttcagaag aggtgctgac acctgatgga
1921 agaccgattc agatgaggat aggaattcac tcaggctccg tgctggctgg agttgttggg
1981 gtgcgaatgc cacgttattg cctgtttgga aataatgtca cactggcaag caaattcgag
2041 tcgggaagtc accctcggcg catcaatgtc agcccaacca cttaccaatt attaaaacga
2101 gaagaaagtt tcacattcat tccgcggtct cgtgaagagc ttccagacaa ctttccaaag
2161 gaaattcctg ggatctgcta tttcctggag gtaaggactg gtccaaagcc accaaagcct
2221 tctctttctt cgtcgagaat aaaaaaggtt tcctacaaca tcggcaccat gttcctccgg
2281 gagacaagcc tctgagacct gctacagatc aaagactcct ccaaaaagca caagcccaga
2341 acatgggtca ccaatggggg gtggaaagag attgtgtctc tttcattgct ttgttgagaa
2401 caagcagcaa aatttctgta ttatgtcagg caataatcct actaaaaggt ggaggtgacc
2461 gctgtcaat
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