LOCUS BC126392 2985 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens phosphorylase, glycogen, muscle, mRNA (cDNA clone
MGC:161670 IMAGE:8992108), complete cds.
ACCESSION BC126392
VERSION BC126392.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2985)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2985)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (22-OCT-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 7 Row: M Column: 2.
FEATURES Location/Qualifiers
source 1..2985
/db_xref="H-InvDB:HIT000388984"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:161670 IMAGE:8992108"
/tissue_type="Pooled, cerebellum, kidney, placenta,
testis, lung, colon, liver, heart, thyroid, bladder,
uterus, PCR rescued clones"
/clone_lib="NIH_MGC_362"
/lab_host="DH10B"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CTTTAACT"
gene 1..2985
/gene="PYGM"
/db_xref="GeneID:5837"
/db_xref="HGNC:HGNC:9726"
/db_xref="MIM:608455"
CDS 400..2928
/gene="PYGM"
/codon_start=1
/product="phosphorylase, glycogen, muscle"
/protein_id="AAI26393.1"
/db_xref="GeneID:5837"
/db_xref="HGNC:HGNC:9726"
/db_xref="MIM:608455"
/translation="MSRPLSDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDR
NVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVN
LALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGI
RYEFGIFNQKISGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGHVEHTSQGAKWVD
TQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENI
SRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTNFDAFPDKV
AIQLNDTHPSLAIPELMRILVDLERMDWDKAWDVTVRTCAYTNHTVLPEALERWPVHL
LETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGS
HAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERI
GEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDI
QVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLV
TAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKF
MLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELR
QVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREW
TRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEAI"
BASE COUNT 670 a 895 c 840 g 580 t
ORIGIN
1 ccatcctggt tcagccactg acagacagct ccccaatcca gcactctgcc tgctccttct
61 tgtatccaac cctccaaaag gcagggacaa gggacagaag accatgtgca aggcctggag
121 gaagtgggtc agcacaggcc tgtgaacaag ccaggccagt cttggcggcc caagtggagc
181 tcacctctcc ctcccctctc ctctcctccc cttgccctca aaatagctcc ctgagctgcc
241 aggccaagac cctcccattg caggggaggc ggggcgtgct gcccagagcc aggctttaaa
301 tccccttgag gctggggagc tccactcctt ggctggaggc agtgctgagg ccgccgtccc
361 ctctaccatc agtccagtcc ggcccgccct cctgcagcca tgtcccggcc cctgtcagac
421 caagagaaaa gaaagcaaat cagtgtgcgt ggcctggccg gcgtggagaa cgtgactgag
481 ctgaaaaaga acttcaaccg gcacctgcat ttcacactcg taaaggaccg caatgtggcc
541 accccacgag actactactt tgctctggcc cataccgtgc gcgaccacct cgtggggcgc
601 tggatccgca cgcagcagca ctactatgag aaggacccca agaggatcta ctacctgtct
661 ttagagttct atatgggacg gacgctacag aacaccatgg tgaacctggc cttagagaat
721 gcctgtgacg aggccaccta ccagctgggc ctggacatgg aggagctgga ggaaattgag
781 gaggatgcgg ggctgggcaa cgggggcctg ggccggctgg cagcctgctt tcttgactcc
841 atggcaacac tgggcctggc cgcctatggc tacgggattc gctatgagtt tgggattttt
901 aaccagaaga tctccggggg ctggcagatg gaggaggccg atgactggct tcgctacggc
961 aacccctggg agaaggcccg gcccgagttc acgctacctg tgcacttcta cggccatgtg
1021 gagcacacca gccagggtgc caagtgggtg gacacacagg tggtactggc catgccctac
1081 gatacgcccg tgcctggcta tcgcaacaat gttgtcaaca ccatgcgcct ctggtctgcc
1141 aaggctccca atgacttcaa cctcaaggac ttcaatgtcg gtggctacat ccaggctgtg
1201 ttggaccgaa acctggcgga gaacatctct cgtgtcctgt accccaatga taatttcttc
1261 gaagggaagg agctgcggct gaagcaggag tatttcgtgg tggctgccac cctccaggac
1321 atcatccgtc gcttcaagtc ttccaagttc ggctgccgtg atcccgtgcg cacgaacttc
1381 gatgccttcc cagataaggt ggccatccag ctcaatgaca cccacccctc cctggccatc
1441 cccgagctga tgaggatcct ggtggacctg gaacggatgg actgggacaa ggcgtgggat
1501 gtgacagtga ggacctgtgc ctacaccaac cacacggtgc tgcccgaggc cctggagcgc
1561 tggccggtgc acctcttgga gacgctgctg ccgcggcacc tccagatcat ctacgagatc
1621 aaccagcgct tcctcaaccg ggtggcggcc gcattcccag gggacgtaga ccggctgcgg
1681 cgcatgtcgc tggtggagga gggcgcagtg aagcgcatca acatggcaca cctgtgcatc
1741 gcggggtcgc acgccgtcaa cggcgtggcg cgcatccact ccgagatcct caagaagacc
1801 atcttcaaag acttctatga gctggagcct cataagttcc agaataagac caacggcatc
1861 acccctcggc gctggctggt tctgtgtaac cccgggctgg cagaggtcat tgctgagcgc
1921 atcggggagg acttcatctc tgacctggac cagctgcgca aactgctctc ctttgtggat
1981 gatgaagctt tcattcggga tgtggccaaa gtgaagcagg aaaacaagtt gaagtttgct
2041 gcctacctag agagggaata caaagtccac atcaacccca actcactctt cgacatccag
2101 gtgaagcgga ttcacgaata taaacgacag ctcctcaact gcctccatgt catcaccctg
2161 tacaaccgca tcaagaggga gcccaataag ttttttgtgc ctcggactgt gatgattgga
2221 gggaaggctg cacctgggta ccacatggcc aagatgatca tcagactcgt cacagccatc
2281 ggggatgtgg tcaaccatga cccggcagtg ggtgaccgcc tccgtgtcat cttcctggag
2341 aactaccgag tctcactggc cgagaaagtg atcccagctg cagacctctc tgagcagatc
2401 tccactgcgg gcactgaagc ctcaggcacc ggcaacatga agttcatgct caacggggct
2461 ctgaccattg gcaccatgga cggggccaat gtggagatgg cagaagaggc gggagaggaa
2521 aacttcttca tctttggcat gcgggtggag gatgtggata agcttgacca aagagggtac
2581 aatgcccagg agtactacga tcgcattcct gagcttcggc aggtcattga gcagctgagc
2641 agtggcttct tctcccccaa acagcccgac ctgttcaagg acattgtcaa tatgctcatg
2701 caccatgacc ggtttaaagt cttcgcagat tatgaagact acattaaatg ccaggagaaa
2761 gtcagcgcct tgtacaagaa cccaagagag tggacgcgga tggtgatccg gaacatagcc
2821 acctctggca agttctccag tgaccgcacc attgcccagt atgcccggga gatctggggt
2881 gtggagcctt cccgccagcg cctgccagcc ccggatgagg ccatctgagc ctccagacca
2941 gaccccaaac cagcccttga gtctgtcaca ctctcttggg ccagc
//