LOCUS BC125105 2473 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens ras homolog gene family, member T1, mRNA (cDNA clone
MGC:149787 IMAGE:40118341), complete cds.
ACCESSION BC125105
VERSION BC125105.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2473)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2473)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-OCT-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAM Plate: 27 Row: l Column: 22.
FEATURES Location/Qualifiers
source 1..2473
/db_xref="H-InvDB:HIT000388558"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:149787 IMAGE:40118341"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_283"
/note="Vector: pCR-Blunt II-TOPO with reversed insert;
Clone identification sequence tag: GTAAAACT sequenced from
the forward primer"
gene 1..2473
/gene="RHOT1"
/gene_synonym="MIRO-1"
/db_xref="GeneID:55288"
/db_xref="HGNC:HGNC:21168"
CDS 69..1925
/gene="RHOT1"
/gene_synonym="MIRO-1"
/codon_start=1
/product="ras homolog gene family, member T1"
/protein_id="AAI25106.1"
/db_xref="GeneID:55288"
/db_xref="HGNC:HGNC:21168"
/translation="MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPA
DVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLIN
ERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFY
YAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFN
TPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGY
DDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFK
VFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQ
ESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIR
EDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCA
RIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPS
KDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR"
BASE COUNT 767 a 475 c 514 g 717 t
ORIGIN
1 gggtgctcct ggtgagagga gtccactccg tgcgtgcggg cggaggccgg cccccgagag
61 ccgccgacat gaagaaagac gtgcggatcc tgctggtggg agaacctaga gttgggaaga
121 catcactgat tatgtctctg gtcagtgaag aatttccaga agaggttcct ccccgggcag
181 aagaaatcac cattccagct gatgtcaccc cagagagagt tccaacacac attgtagatt
241 actcagaagc agaacagagt gatgaacaac ttcatcaaga aatatctcag gctaatgtca
301 tctgtatagt gtatgccgtt aacaacaagc attctattga taaggtaaca agtcgatgga
361 ttcctctcat aaatgaaaga acagacaaag acagcaggct gcctttaata ttggttggga
421 acaaatctga tctggtggaa tatagtagta tggagaccat ccttcctatt atgaaccagt
481 atacagaaat agaaacctgt gtggagtgtt cagcgaaaaa cctgaagaac atatcagagc
541 tcttttatta cgcacagaaa gctgttcttc atcctacagg gcccctgtac tgcccagagg
601 agaaggagat gaaaccagct tgtataaaag cccttactcg tatatttaaa atatctgatc
661 aagataatga tggtactctc aatgatgctg aactcaactt ctttcagagg atttgtttca
721 acactccatt agctcctcaa gctctggagg atgtcaagaa tgtagtcaga aaacatataa
781 gtgatggtgt ggctgacagt gggttgaccc tgaaaggttt tctcttttta cacacacttt
841 ttatccagag agggagacac gaaactactt ggactgtgct tcgacgattt ggttatgatg
901 atgacctgga tttgacacct gaatatttgt tccccctgct gaaaatacct cctgattgca
961 ctactgaatt aaatcatcat gcatatttat ttctccaaag cacctttgac aagcatgatt
1021 tggatagaga ctgtgctttg tcacctgatg agcttaaaga tttatttaaa gttttccctt
1081 acataccttg ggggccagat gtgaataaca cagtttgtac caatgaaaga ggctggataa
1141 cctaccaggg attcctttcc cagtggacgc tcacgactta tttagatgta cagcggtgcc
1201 tggaatattt gggctatcta ggctattcaa tattgactga gcaagagtct caagcttcag
1261 ctgttacagt gacaagagat aaaaagatag acctgcagaa aaaacaaact caaagaaatg
1321 tgttcagatg taatgtaatt ggagtgaaaa actgtgggaa aagtggagtt cttcaggctc
1381 ttcttggaag aaacttaatg aggcagaaga aaattcgtga agatcataaa tcctactatg
1441 cgattaacac tgtttatgta tatggacaag agaaatactt gttgttgcat gatatctcag
1501 aatcggaatt tctaactgaa gctgaaatca tttgtgatgt tgtatgcctg gtatatgatg
1561 tcagcaatcc caaatccttt gaatactgtg ccaggatttt taagcaacac tttatggaca
1621 gcagaatacc ttgcttaatc gtagctgcaa agtcagacct gcatgaagtt aaacaagaat
1681 acagtatttc acctactgat ttctgcagga aacacaaaat gcctccacca caagccttca
1741 cttgcaatac tgctgatgcc cccagtaagg atatctttgt taaattgaca acaatggcca
1801 tgtatccgca cgtgacacaa gctgacctca agagctccac gttttggctt cgagcaagtt
1861 ttggtgctac tgtttttgca gttttgggct ttgctatgta caaagcatta ttgaaacagc
1921 gatgatataa aaagaaatac tgtccctacc aaaaacaaat acttttatgt acattctgaa
1981 tgctttaagt tctgctagaa ttattgagat atttatacat gcagagttac tttattaata
2041 tttgtaattc atgcataaga gtattttaat gatagttata actgcagtat tggctagcat
2101 atggaaagaa aacagctaac agccaaacta aaatggctaa attccagagg ccaaaaggga
2161 atattttgta aatatatgta catattcagg caagatatgg tctcccaagc tgagttctag
2221 aaatgatgtt tctagacatt tctaagtggt attgttagtg ctcacttggc tcactcttct
2281 aggtttaagt tagcccagag attgtattta ctcatggatc actttattta tttcacattt
2341 actcagaatg atcctttggg ttctataagg acataaggta caatttgcca ttgtctctcc
2401 atttttaaaa acatacaagt cagtgtcagc ttaccaacat gacatttttt cagtcagttg
2461 tggtaggcca gcc
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