LOCUS BC119656 1253 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 2, mRNA (cDNA
clone MGC:149517 IMAGE:40115940), complete cds.
ACCESSION BC119656
VERSION BC119656.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1253)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1253)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-JUL-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAM Plate: 27 Row: b Column: 15.
FEATURES Location/Qualifiers
source 1..1253
/db_xref="H-InvDB:HIT000387975"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:149517 IMAGE:40115940"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_282"
/note="Vector: pCR-Blunt II-TOPO; Clone identification
sequence tag: TTTTTTC sequenced from the forward primer"
gene 1..1253
/gene="PDHA2"
/db_xref="GeneID:5161"
/db_xref="HGNC:HGNC:8807"
/db_xref="MIM:179061"
CDS 32..1198
/gene="PDHA2"
/codon_start=1
/product="pyruvate dehydrogenase (lipoamide) alpha 2"
/protein_id="AAI19657.1"
/db_xref="GeneID:5161"
/db_xref="HGNC:HGNC:8807"
/db_xref="MIM:179061"
/translation="MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLL
EEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGL
EAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYG
GNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVF
ICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKG
PILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELK
EIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS"
BASE COUNT 341 a 257 c 337 g 318 t
ORIGIN
1 ccgctgccat ctacagcact ccgtgaagaa tatgctggcc gccttcatct cccgcgtgtt
61 gaggcgagtt gcccagaaat cagctcgcag agtgctggtg gcatcccgta actcctcaaa
121 tgacgctaca tttgaaatta agaaatgtga tctttatctg ttggaagagg gtccccctgt
181 cactacagtg ctcactaggg cggaggggct taaatactac aggatgatgc tgactgttcg
241 ccgcatggaa ttgaaggcag atcagctgta caaacagaaa ttcattcgcg gtttctgtca
301 cctgtgcgat ggtcaggaag cttgttgcgt gggccttgag gccggcataa acccctcgga
361 tcacgtcatt acatcctata gggctcatgg tgtgtgctat actcggggac tttctgtccg
421 atccattctc gcagagctga cgggaagaag aggaggttgt gctaaaggaa aaggaggatc
481 gatgcatatg tataccaaga acttctatgg gggcaatggc atcgtcggtg cacagggccc
541 cctgggcgct ggcattgctc tggcctgtaa atataaagga aacgatgaga tctgtttgac
601 tttatatggg gatggcgctg cgaatcaggg gcagatagcc gaagctttca atatggcagc
661 tttatggaaa ttaccttgtg ttttcatctg tgagaataac ctatatggaa tgggaacatc
721 tactgagaga gcagcagcca gccctgatta ctacaagagg ggcaatttta tccctgggct
781 aaaggtcgat ggaatggatg ttctgtgtgt tcgtgaggca acaaaatttg cagctaacta
841 ctgtagatct ggaaaggggc ccatactgat ggagctgcaa acctaccgtt atcatggaca
901 cagtatgagt gatcctggag tcagttatcg tacacgagaa gaaattcagg aagtaagaag
961 taagagggat cctataataa ttctccaaga tagaatggta aacagcaagc tcgccactgt
1021 ggaagaatta aaggaaattg gggctgaggt gaggaaagaa attgatgatg ctgcccagtt
1081 tgctaccact gatcctgagc cacatttgga agaattaggc catcacatct acagcagtga
1141 ttcatctttt gaagttcgtg gtgcaaatcc atggatcaag tttaagtccg tcagttaaag
1201 ggaggctacg tgtgaattta tcatcagtct ctcaatggaa tgttcatggt caa
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