LOCUS BC117178 4139 bp mRNA linear HUM 26-JUN-2006
DEFINITION Homo sapiens NPC1 (Niemann-Pick disease, type C1, gene)-like 1,
mRNA (cDNA clone MGC:150787 IMAGE:40125729), complete cds.
ACCESSION BC117178
VERSION BC117178.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4139)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4139)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-MAY-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 6 Row: C Column: 18.
FEATURES Location/Qualifiers
source 1..4139
/db_xref="H-InvDB:HIT000387468"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:150787 IMAGE:40125729"
/tissue_type="Liver, PCR rescued clones"
/clone_lib="NIH_MGC_293"
/note="Vector: pCR-XL-TOPO with reversed insert; Clone
identification sequence tag: CGCACGGC"
gene 1..4139
/gene="NPC1L1"
/db_xref="GeneID:29881"
/db_xref="HGNC:HGNC:7898"
/db_xref="MIM:608010"
CDS 55..4053
/gene="NPC1L1"
/codon_start=1
/product="NPC1L1 protein"
/protein_id="AAI17179.1"
/db_xref="GeneID:29881"
/db_xref="HGNC:HGNC:7898"
/db_xref="MIM:608010"
/translation="MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPE
LSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEAS
LSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFY
QHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDI
TFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTF
YLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTH
TLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARS
EKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQER
LRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQT
LMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSE
AEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDE
INRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMA
AMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRAL
GRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLD
SKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLAL
FGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEA
GMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYIS
GPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLA
AYSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDP
AFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIV
MILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEA
TISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVIL
SYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGA
ISNFLPNNGRQF"
BASE COUNT 743 a 1365 c 1096 g 935 t
ORIGIN
1 tggctgttcc tgaggcctgg cctggctccc cgctgacccc ttcccagacc tgggatggcg
61 gaggccggcc tgaggggctg gctgctgtgg gccctgctcc tgcgcttggc ccagagtgag
121 ccttacacaa ccatccacca gcctggctac tgcgccttct atgacgaatg tgggaagaac
181 ccagagctgt ctggaagcct catgacactc tccaacgtgt cctgcctgtc caacacgccg
241 gcccgcaaga tcacaggtga tcacctgatc ctattacaga agatctgccc ccgcctctac
301 accggcccca acacccaagc ctgctgctcc gccaagcagc tggtatcact ggaagcgagt
361 ctgtcgatca ccaaggccct cctcacccgc tgcccagcct gctctgacaa ttttgtgaac
421 ctgcactgcc acaacacgtg cagccccaat cagagcctct tcatcaatgt gacccgcgtg
481 gcccagctag gggctggaca actcccagct gtggtggcct atgaggcctt ctaccagcat
541 agctttgccg agcagagcta tgactcctgc agccgtgtgc gcgtccctgc agctgccacg
601 ctggctgtgg gcaccatgtg tggcgtgtat ggctctgccc tttgcaatgc ccagcgctgg
661 ctcaacttcc agggagacac aggcaatggt ctggccccac tggacatcac cttccacctc
721 ttggagcctg gccaggccgt ggggagtggg attcagcctc tgaatgaggg ggttgcacgt
781 tgcaatgagt cccaaggtga cgacgtggcg acctgctcct gccaagactg tgctgcatcc
841 tgtcctgcca tagcccgccc ccaggccctc gactccacct tctacctggg ccagatgccg
901 ggcagtctgg tcctcatcat catcctctgc tctgtcttcg ctgtggtcac catcctgctt
961 gtgggattcc gtgtggcccc cgccagggac aaaagcaaga tggtggaccc caagaagggc
1021 accagcctct ctgacaagct cagcttctcc acccacaccc tccttggcca gttcttccag
1081 ggctggggca cgtgggtggc ttcgtggcct ctgaccatct tggtgctatc tgtcatcccg
1141 gtggtggcct tggcagcggg cctggtcttt acagaactca ctacggaccc cgtggagctg
1201 tggtcggccc ccaacagcca agcccggagt gagaaagctt tccatgacca gcatttcggc
1261 cccttcttcc gaaccaacca ggtgatcctg acggctccta accggtccag ctacaggtat
1321 gactctctgc tgctggggcc caagaacttc agcggaatcc tggacctgga cttgctgctg
1381 gagctgctag agctgcagga gaggctgcgg cacctccagg tatggtcgcc cgaagcacag
1441 cgcaacatct ccctgcagga catctgctac gcccccctca atccggacaa taccagtctc
1501 tacgactgct gcatcaacag cctcctgcag tatttccaga acaaccgcac gctcctgctg
1561 ctcacagcca accagacact gatggggcag acctcccaag tcgactggaa ggaccatttt
1621 ctgtactgtg ccaatgcccc gctcaccttc aaggatggca cagccctggc cctgagctgc
1681 atggctgact acggggcccc tgtcttcccc ttccttgcca ttggggggta caaaggaaag
1741 gactattctg aggcagaggc cctgatcatg acgttctccc tcaacaatta ccctgccggg
1801 gacccccgtc tggcccaggc caagctgtgg gaggaggcct tcttagagga aatgcgagcc
1861 ttccagcgtc ggatggctgg catgttccag gtcacgttca tggctgagcg ctctctggaa
1921 gacgagatca atcgcaccac agctgaagac ctgcccatct ttgccaccag ctacattgtc
1981 atattcctgt acatctctct ggccctgggc agctattcca gctggagccg agtgatggtg
2041 gactccaagg ccacgctggg cctcggcggg gtggccgtgg tcctgggagc agtcatggct
2101 gccatgggct tcttctccta cttgggtatc cgctcctccc tggtcatcct gcaagtggtt
2161 cctttcctgg tgctgtccgt gggggctgat aacatcttca tctttgttct cgagtaccag
2221 aggctgcccc ggaggcctgg ggagccacga gaggtccaca ttgggcgagc cctaggcagg
2281 gtggctccca gcatgctgtt gtgcagcctc tctgaggcca tctgcttctt cctaggggcc
2341 ctgaccccca tgccagctgt gcggaccttt gccctgacct ctggccttgc agtgatcctt
2401 gacttcctcc tgcagatgtc agcctttgtg gccctgctct ccctggacag caagaggcag
2461 gaggcctccc ggttggacgt ctgctgctgt gtcaagcccc aggagctgcc cccgcctggc
2521 cagggagagg ggctcctgct tggcttcttc caaaaggctt atgccccctt cctgctgcac
2581 tggatcactc gaggtgttgt gctgctgctg tttctcgccc tgttcggagt gagcctctac
2641 tccatgtgcc acatcagcgt gggactggac caggagctgg ccctgcccaa ggactcgtac
2701 ctgcttgact atttcctctt tctgaaccgc tacttcgagg tgggggcccc ggtgtacttt
2761 gttaccacct tgggctacaa cttctccagc gaggctggga tgaatgccat ctgctccagt
2821 gcaggctgca acaacttctc cttcacccag aagatccagt atgccacaga gttccctgag
2881 cagtcttacc tggccatccc tgcctcctcc tgggtggatg acttcattga ctggctgacc
2941 ccgtcctcct gctgccgcct ttatatatct ggccccaata aggacaagtt ctgcccctcg
3001 accgtcaact ctctgaactg cctaaagaac tgcatgagca tcacgatggg ctctgtgagg
3061 ccctcggtgg agcagttcca taagtatctt ccctggttcc tgaacgaccg gcccaacatc
3121 aaatgtccca aaggcggcct ggcagcatac agcacctctg tgaacttgac ttcagatggc
3181 caggttttag cctccaggtt catggcctat cacaagcccc tgaaaaactc acaggattac
3241 acagaagctc tgcgggcagc tcgagagctg gcagccaaca tcactgctga cctgcggaaa
3301 gtgcctggaa cagacccggc ttttgaggtc ttcccctaca cgatcaccaa tgtgttttat
3361 gagcagtacc tgaccatcct ccctgagggg ctcttcatgc tcagcctctg ccttgtgccc
3421 accttcgctg tctcctgcct cctgctgggc ctggacctgc gctccggcct cctcaacctg
3481 ctctccattg tcatgatcct cgtggacact gtcggcttca tggccctgtg gggcatcagt
3541 tacaatgctg tgtccctcat caacctggtc tcggcggtgg gcatgtctgt ggagtttgtg
3601 tcccacatta cccgctcctt tgccatcagc accaagccca cctggctgga gagggccaaa
3661 gaggccacca tctctatggg aagtgcggtg tttgcaggtg tggccatgac caacctgcct
3721 ggcatccttg tcctgggcct cgccaaggcc cagctcattc agatcttctt cttccgcctc
3781 aacctcctga tcactctgct gggcctgctg catggcttgg tcttcctgcc cgtcatcctc
3841 agctatgtgg ggcctgacgt caacccggct ctggcactgg agcagaagcg ggctgaggag
3901 gcggtggcag cagtcatggt ggcctcttgc ccaaatcacc cctcccgagt ctccacagct
3961 gacaacatct atgtcaacca cagctttgaa ggttctatca aaggtgctgg tgccatcagc
4021 aacttcttgc ccaacaatgg gcggcagttc tgatacagcc agaggccctg tctaggctct
4081 atggccctga accaaagggt tatggggatc ttccttgtga ctgccccttg acacacgcc
//