LOCUS BC117151 2206 bp mRNA linear HUM 26-JUN-2006
DEFINITION Homo sapiens RAS guanyl releasing protein 2 (calcium and
DAG-regulated), mRNA (cDNA clone MGC:150760 IMAGE:40125702),
complete cds.
ACCESSION BC117151
VERSION BC117151.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2206)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2206)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-MAY-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 6 Row: B Column: 15.
FEATURES Location/Qualifiers
source 1..2206
/db_xref="H-InvDB:HIT000387441"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:150760 IMAGE:40125702"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_311"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: GCTCCATC"
gene 1..2206
/gene="RASGRP2"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="CDC25L"
/db_xref="GeneID:10235"
/db_xref="HGNC:HGNC:9879"
/db_xref="MIM:605577"
CDS 252..2081
/gene="RASGRP2"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="CDC25L"
/codon_start=1
/product="RAS guanyl releasing protein 2 (calcium and
DAG-regulated)"
/protein_id="AAI17152.1"
/db_xref="GeneID:10235"
/db_xref="HGNC:HGNC:9879"
/db_xref="MIM:605577"
/translation="MAGTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHP
WYIPSSQLAAKLLHIYQQSRKDNSNSLQVKTCHLVRYWISAFPAEFDLNPELAEQIKE
LKALLDQEGNRRHSSLIDIDSVPTYKWKRQVTQRNPVGQKKRKMSLLFDHLEPMELAE
HLTYLEYRSFCKILFQDYHSFVTHGCTVDNPVLERFISLFNSVSQWVQLMILSKPTAP
QRALVITHFVHVAEKLLQLQNFNTLMAVVGGLSHSSISRLKETHSHVSPETIKLWEGL
TELVTATGNYGNYRRRLAACVGFRFPILGVHLKDLVALQLALPDWLDPARTRLNGAKM
KQLFSILEELAMVTSLRPPVQANPDLLSLLTVSLDQYQTEDELYQLSLQREPRSKSSP
TSPTSCTPPPRPPVLEEWTSAAKPKLDQALVVEHIEKMVESVFRNFDVDGDGHISQEE
FQIIRGNFPYLSAFGDLDQNQDGCISREEMVSYFLRSSSVLGGRMGFVHNFQESNSLR
PVACRHCKALILGIYKQGLKCRACGVNCHKQCKDRLSVECRRRAQSVSLEGSAPSPSP
MHSHHHRAFSFSLPRPGRRGSRPPEIREEEVQTVEDGVFDIHL"
BASE COUNT 443 a 708 c 649 g 406 t
ORIGIN
1 atttcattcc tcgctcccca caggtccctc tccccaaaat attcccatct tgtcctagcc
61 catcccccag actatctcaa ggaccagctg tccccacgcc cccgacctcc actaggcctg
121 tgccacccgc tgcctgcagg aagacgcccg gtcccgggcc gggttagccc catgggaacg
181 gggttcggtc cgagcccggt gggaggctcc cggagcgcag cctgggccca gcccaccccg
241 cgccggcggc catggcaggc accctggacc tggacaaggg ctgcacggtg gaggagctgc
301 tccgcgggtg catcgaagcc ttcgatgact ccgggaaggt gcgggacccg cagctggtgc
361 gcatgttcct catgatgcac ccctggtaca tcccctcctc tcagctggcg gccaagctgc
421 tccacatcta ccaacaatcc cggaaggaca actccaattc cctgcaggtg aaaacgtgcc
481 acctggtcag gtactggatc tccgccttcc cagcggagtt tgacttgaac ccggagttgg
541 ctgagcagat caaggagctg aaggctctgc tagaccaaga agggaaccga cggcacagca
601 gcctaatcga catagacagc gtccctacct acaagtggaa gcggcaggtg actcagcgga
661 accctgtggg acagaaaaag cgcaagatgt ccctgttgtt tgaccacctg gagcccatgg
721 agctggcgga gcatctcacc tacttggagt atcgctcctt ctgcaagatc ctgtttcagg
781 actatcacag tttcgtgact catggctgca ctgtggacaa ccccgtcctg gagcggttca
841 tctccctctt caacagcgtc tcacagtggg tgcagctcat gatcctcagc aaacccacag
901 ccccgcagcg ggccctggtc atcacacact ttgtccacgt ggcggagaag ctgctacagc
961 tgcagaactt caacacgctg atggcagtgg tcgggggcct gagccacagc tccatctccc
1021 gcctcaagga gacccacagc cacgttagcc ctgagaccat caagctctgg gagggtctca
1081 cggaactagt gacggcgaca ggcaactatg gcaactaccg gcgtcggctg gcagcctgtg
1141 tgggcttccg cttcccgatc ctgggtgtgc acctcaagga cctggtggcc ctgcagctgg
1201 cactgcctga ctggctggac ccagcccgga cccggctcaa cggggccaag atgaagcagc
1261 tctttagcat cctggaggag ctggccatgg tgaccagcct gcggccacca gtacaggcca
1321 accccgacct gctgagcctg ctcacggtgt ctctggatca gtatcagacg gaggatgagc
1381 tgtaccagct gtccctgcag cgggagccgc gctccaagtc ctcgccaacc agccccacga
1441 gttgcacccc accaccccgg cccccggtac tggaggagtg gacctcggct gccaaaccca
1501 agctggatca ggccctcgtg gtggagcaca tcgagaagat ggtggagtct gtgttccgga
1561 actttgacgt cgatggggat ggccacatct cacaggaaga attccagatc atccgtggga
1621 acttccctta cctcagcgcc tttggggacc tcgaccagaa ccaggatggc tgcatcagca
1681 gggaggagat ggtttcctat ttcctgcgct ccagctctgt gttggggggg cgcatgggct
1741 tcgtacacaa cttccaggag agcaactcct tgcgccccgt cgcctgccgc cactgcaaag
1801 ccctgatcct gggcatctac aagcagggcc tcaaatgccg agcctgtgga gtgaactgcc
1861 acaagcagtg caaggatcgc ctgtcagttg agtgtcggcg cagggcccag agtgtgagcc
1921 tggaggggtc tgcaccctca ccctcaccca tgcacagcca ccatcaccgc gccttcagct
1981 tctctctgcc ccgccctggc aggcgaggct ccaggcctcc agagatccgt gaggaggagg
2041 tacagacggt ggaggatggg gtgtttgaca tccacttgta atagatgctg tggttggatc
2101 aaggactcat tcctgccttg gagaaaatac ttcaaccaga gcagggagcc tgggggtgtc
2161 ggggcaggag gctggggatg ggggtgggat atgagggtgg catgca
//