LOCUS BC113658 5200 bp mRNA linear HUM 29-JUN-2006
DEFINITION Homo sapiens phosphoinositide-3-kinase, class 2, alpha polypeptide,
mRNA (cDNA clone MGC:142218 IMAGE:8322710), complete cds.
ACCESSION BC113658
VERSION BC113658.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5200)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5200)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-FEB-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 5 Row: m Column: 12.
FEATURES Location/Qualifiers
source 1..5200
/db_xref="H-InvDB:HIT000387111"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:142218 IMAGE:8322710"
/tissue_type="Lung, PCR rescued clones"
/clone_lib="NIH_MGC_300"
/note="Vector: pCR-XL-TOPO; Clone identification sequence
tag: ACACACAC"
gene 1..5200
/gene="PIK3C2A"
/gene_synonym="CPK"
/gene_synonym="PI3-K-C2(ALPHA)"
/gene_synonym="PI3-K-C2A"
/db_xref="GeneID:5286"
/db_xref="HGNC:HGNC:8971"
/db_xref="MIM:603601"
CDS 67..5127
/gene="PIK3C2A"
/gene_synonym="CPK"
/gene_synonym="PI3-K-C2(ALPHA)"
/gene_synonym="PI3-K-C2A"
/codon_start=1
/product="phosphoinositide-3-kinase, class 2, alpha
polypeptide"
/protein_id="AAI13659.1"
/db_xref="GeneID:5286"
/db_xref="HGNC:HGNC:8971"
/db_xref="MIM:603601"
/translation="MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQK
DRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEK
LLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPS
TYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPV
VSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPL
SKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVT
RSQSLNIRTTQLAKAQGHISQKDPNGTSSLPTGSSLLQEVEVQNEEMAAFCRSITKLK
TKFPYTNHRTNPGYLLSPVTAQRNICGENASVKVSIDIEGFQLPVTFTCDVSSTVEII
IMQALCWVHDDLNQVDVGSYVLKVCGQEEVLQNNHCLGSHEHIQNCRKWDTEIRLQLL
TFSAMCQNLARTAEDDETPVDLNKHLYQIEKPCKEAMTRHPVEELLDSYHNQVELALQ
IENQHRAVDQVIKAVRKICSALDGVETLAITESVKKLKRAVNLPRSKTADVTSLFGGE
DTSRSSTRGSLNPENPVQVSINQLTAAIYDLLRLHANSGRSPTDCAQSSKSVKEAWTT
TEQLQFTIFAAHGISSNWVSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLIK
WDELIIFPIQISQLPLESVLHLTLFGILNQSSGSSPDSNKQRKGPEALGKVSLPLFDF
KRFLTCGTKLLYLWTSSHTNSVPGTVTKKGYVMERIVLQVDFPSPAFDIIYTTPQVDR
SIIQQHNLETLENDIKGKLLDILHKDSSLGLSKEDKAFLWEKRYYCFKHPNCLPKILA
SAPNWKWVNLAKTYSLLHQWPALYPLIALELLDSKFADQEVRSLAVTWIEAISDDELT
DLLPQFVQALKYEIYLNSSLVQFLLSRALGNIQIAHNLYWLLKDALHDVQFSTRYEHV
LGALLSVGGKRLREELLKQTKLVQLLGGVAEKVRQASGSARQVVLQRSMERVQSFFQK
NKCRLPLKPSLVAKELNIKSCSFFSSNAVPLKVTMVNADPMGEEINVMFKVGEDLRQD
MLALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDRGMVELVPASDTLRKIQVEYGVTG
SFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRSTG
HMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAY
NLIRKQTNLFLNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESS
LGSIATKFNFFIHNLAQLRFSGLPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKY
NPDKHYIYVVRILREGQIEPSFVFRTFDEFQELHNKLSIIFPLWKLPGFPNRMVLGRT
HIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEKAEGIARSADAGSF
SPTPGQIGGAVKLSISYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDNHKTSKR
KTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGVTLPLKDF
NLSKETVKWYQLTAATYL"
BASE COUNT 1685 a 1015 c 1033 g 1467 t
ORIGIN
1 ggccttgcta ttttggaagc tacaagaaaa aaatagagga aggaaggttt ggtcttttta
61 gtggacatgg ctcagatatc tagcaacagc ggatttaaag aatgtccatc ttcacatccg
121 gaaccaacaa gagcaaaaga tgtggacaaa gaagaagcat tacagatgga agcagaggct
181 ttagcaaaac tgcaaaagga tagacaagtg actgacaatc agagaggctt tgagttgtca
241 agcagcacca gaaaaaaagc acaggtttat aacaagcagg attatgatct catggtgttt
301 cctgaatcag attcccaaaa aagagcatta gatattgatg tagaaaagct cacccaagct
361 gaacttgaga aactattgct ggatgacagt ttcgagacta aaaaaacacc tgtattacca
421 gttactccta ttctgagccc ttccttttca gcacagctct attttagacc tactattcag
481 agaggacagt ggccacctgg attacctggg ccttccactt atgctttacc ttctatttat
541 ccttctactt acagtaaaca ggctgcattc caaaatggct tcaatccaag aatgcccact
601 tttccatcta cagaacctat atatttaagt cttccgggac aatctccata tttctcatat
661 cctttgacac ctgccacacc ctttcatcca caaggaagct tacctatcta tcgtccagta
721 gtcagtactg acatggcaaa actatttgac aaaatagcta gtacatcaga atttttaaaa
781 aatgggaaag caaggactga tttggagata acagattcaa aagtcagcaa tctacaggta
841 tctccaaagt ctgaggatat cagtaaattt gactggttag acttggatcc tctaagtaag
901 cctaaggtgg ataatgtgga ggtattagac catgaggaag agaaaaatgt ttcaagtttg
961 ctagcaaagg atccttggga tgctgttctt cttgaagaga gatcgacagc aaattgtcat
1021 cttgaaagaa aggtgaatgg aaaatccctt tctgtggcaa ctgttacaag aagccagtct
1081 ttaaatattc gaacaactca gcttgcaaaa gcccagggcc atatatctca gaaagaccca
1141 aatgggacca gtagtttgcc aactggaagt tctcttcttc aagaagttga agtacagaat
1201 gaggagatgg cagctttttg tcgatccatt acaaaattga agaccaaatt tccatatacc
1261 aatcaccgca caaacccagg ctatttgtta agtccagtca cagcgcaaag aaacatatgc
1321 ggagaaaatg ctagtgtgaa ggtctccatt gacattgaag gatttcagct accagttact
1381 tttacgtgtg atgtgagttc tactgtagaa atcattataa tgcaagccct ttgctgggta
1441 catgatgact tgaatcaagt agatgttggc agctatgttc taaaagtttg tggtcaagag
1501 gaagtgctgc agaataatca ttgccttgga agtcatgagc atattcaaaa ctgtcgaaaa
1561 tgggacacag aaattagact acaactcttg accttcagtg caatgtgtca aaatctggcc
1621 cgaacagcag aagatgatga aacacccgtg gatttaaaca aacacctgta tcaaatagaa
1681 aaaccttgca aagaagccat gacgagacac cctgttgaag aactcttaga ttcttatcac
1741 aaccaagtag aactggctct tcaaattgaa aaccaacacc gagcagtaga tcaagtaatt
1801 aaagctgtaa gaaaaatctg tagtgcttta gatggtgtcg agactcttgc cattacagaa
1861 tcagtaaaga agctaaagag agcagttaat cttccaagga gtaaaactgc tgatgtgact
1921 tctttgtttg gaggagaaga cactagcagg agttcaacta ggggctcact taatcctgaa
1981 aatcctgttc aagtaagcat aaaccaatta actgcagcaa tttatgatct tctcagactc
2041 catgcaaatt ctggtaggag tcctacagac tgtgcccaaa gtagcaagag tgtcaaggaa
2101 gcatggacta caacagagca gctccagttt actatttttg ctgctcatgg aatttcaagt
2161 aattgggtat caaattatga aaaatactac ttgatatgtt cactgtctca caatggaaag
2221 gatcttttta aacctattca atcaaagaag gttggcactt acaagaattt cttctatctt
2281 attaaatggg atgaactaat catttttcct atccagatat cacaattgcc attagaatca
2341 gttcttcacc ttactctttt tggaatttta aatcagagca gtggaagttc ccctgattct
2401 aataagcaga gaaagggacc agaagctttg ggcaaagttt ctttacctct ttttgacttt
2461 aaacggtttt taacatgtgg aactaaactt ctatatcttt ggacttcatc acatacaaat
2521 tctgttcctg gaacagttac caaaaaagga tatgtcatgg aaagaatagt gctacaggtt
2581 gattttcctt ctcctgcatt tgatattatt tatacaactc ctcaagttga cagaagcatt
2641 atacagcaac ataacttaga aacactagag aatgatataa aagggaaact tcttgatatt
2701 cttcataaag actcatcact tggactttct aaagaagata aagctttttt atgggagaaa
2761 cgttattatt gcttcaaaca cccaaattgt cttcctaaaa tattagcaag cgccccaaac
2821 tggaaatggg ttaatcttgc caaaacttac tcattgcttc accagtggcc tgcattgtac
2881 ccactaattg cattggaact tcttgattca aaatttgctg atcaggaagt aagatcccta
2941 gctgtgacct ggattgaggc cattagtgat gatgagctaa cagatcttct tccacagttt
3001 gtacaagctt tgaaatatga aatttacttg aatagttcat tagtgcaatt ccttttgtcc
3061 agggcattgg gaaatatcca gatagcacac aatttatatt ggcttctcaa agatgccctg
3121 catgatgtac agtttagtac ccgatacgaa catgttttgg gtgctctcct gtcagtagga
3181 ggaaaacgac ttagagaaga acttctaaaa cagacgaaac ttgtacagct tttaggagga
3241 gtagcagaaa aagtaaggca ggctagtgga tcagccagac aggttgttct ccaaagaagt
3301 atggaacgag tacagtcctt ttttcagaaa aataaatgcc gtctccctct caagccaagt
3361 ctagtggcaa aagaattaaa tattaagtcg tgttccttct tcagttctaa tgctgtcccc
3421 ctaaaagtca caatggtgaa tgctgaccct atgggagaag aaattaatgt catgtttaag
3481 gttggtgaag atcttcggca agatatgtta gctttacaga tgataaagat tatggataag
3541 atctggctta aagaaggact agatctgagg atggtaattt tcaaatgtct ctcaactggc
3601 agagatcgag gcatggtgga gctggttcct gcttccgata ccctcaggaa aatccaagtg
3661 gaatatggtg tgacaggatc ctttaaagat aaaccacttg cagagtggct aaggaaatac
3721 aatccctctg aagaagaata tgaaaaggct tcagagaact ttatctattc ctgtgctgga
3781 tgctgtgtag ccacctatgt tttaggcatc tgtgatcgac acaatgacaa tataatgctt
3841 cgaagcacgg gacacatgtt tcacattgac tttggaaagt ttttgggaca tgcacagatg
3901 tttggcagct tcaaaaggga tcgggctcct tttgtgctga cctctgatat ggcatatgtc
3961 attaatgggg gtgaaaagcc caccattcgt tttcagttgt ttgtggacct ctgctgtcag
4021 gcctacaact tgataagaaa gcagacaaac ctttttctta acctcctttc actgatgatt
4081 ccttcagggt taccagaact tacaagtatt caagatttga aatacgttag agatgcactt
4141 caaccccaaa ctacagacgc agaagctaca attttcttta ctaggcttat tgaatcaagt
4201 ttgggaagca ttgccacaaa gtttaacttc ttcattcaca accttgctca gcttcgtttt
4261 tctggtcttc cttctaatga tgagcccatc ctttcatttt cacctaaaac atactccttt
4321 agacaagatg gtcgaatcaa ggaagtctct gtttttacat atcataagaa atacaaccca
4381 gataaacatt atatttatgt agtccgaatt ttgagggaag gacagattga accatcattt
4441 gtcttccgaa catttgacga atttcaggaa cttcacaata agctcagtat tatttttcca
4501 ctttggaagt taccaggctt tcctaatagg atggttctag gaagaacaca cataaaagat
4561 gtagcagcca aaaggaaaat tgagttaaac agttacttac agagtttgat gaatgcttca
4621 acggatgtag cagagtgtga tcttgtttgt actttcttcc accctttact tcgtgatgag
4681 aaagctgaag ggatagctag gtctgcagat gcaggttcct tcagtcctac tccaggccaa
4741 ataggaggag ctgtgaaatt atccatctct taccgaaatg gtactctttt catcatggtg
4801 atgcatatca aagatcttgt tactgaagat ggagctgacc caaatccata tgtcaaaaca
4861 tacctacttc cagataacca caaaacatcc aaacgtaaaa ccaaaatttc acgaaaaacg
4921 aggaatccga cattcaatga aatgcttgta tacagtggat atagcaaaga aaccctaaga
4981 cagcgagaac ttcaactaag tgtactcagt gcagaatctc tgcgggagaa ttttttcttg
5041 ggtggagtaa ccctgccttt gaaagatttc aacttgagca aagagacggt taaatggtat
5101 cagctgactg cggcaacata cttgtaaact agtgaatgtc tgagctttgg aagcatgaac
5161 agttataaac gtgcatgcat acatgcacac acacacagac
//