LOCUS BC113458 2157 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens myosin light chain kinase, mRNA (cDNA clone
IMAGE:8322510), partial cds.
ACCESSION BC113458
VERSION BC113458.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2157)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2157)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-FEB-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 5 Row: e Column: 4.
FEATURES Location/Qualifiers
source 1..2157
/db_xref="H-InvDB:HIT000386911"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:8322510"
/tissue_type="Brain, cerebral cortex and lung, PCR rescued
clones"
/clone_lib="NIH_MGC_323"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: GGCGGCAT"
gene <1..2157
/gene="MYLK"
/gene_synonym="KRP"
/gene_synonym="MLCK"
/gene_synonym="MLCK1"
/gene_synonym="MLCK108"
/gene_synonym="MLCK210"
/gene_synonym="MSTP083"
/gene_synonym="MYLK1"
/gene_synonym="smMLCK"
/db_xref="GeneID:4638"
/db_xref="HGNC:HGNC:7590"
/db_xref="MIM:600922"
CDS <1..503
/gene="MYLK"
/gene_synonym="KRP"
/gene_synonym="MLCK"
/gene_synonym="MLCK1"
/gene_synonym="MLCK108"
/gene_synonym="MLCK210"
/gene_synonym="MSTP083"
/gene_synonym="MYLK1"
/gene_synonym="smMLCK"
/codon_start=3
/product="MYLK protein"
/protein_id="AAI13459.1"
/db_xref="GeneID:4638"
/db_xref="HGNC:HGNC:7590"
/db_xref="MIM:600922"
/translation="GNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLESEEDV
SQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIR
ESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGE
GEEEEE"
BASE COUNT 631 a 425 c 421 g 680 t
ORIGIN
1 cgggcaatgc tgtgagagcc attggaagac tgtcctctat ggcaatgatc tcagggctca
61 gtggcaggaa atcctcaaca gggtcaccaa ccagcccgct caatgcagaa aaactagaat
121 ctgaagaaga tgtgtcccaa gctttccttg aggctgttgc tgaggaaaag cctcatgtaa
181 aaccctattt ctctaagacc attcgcgatt tagaagttgt ggagggaagt gctgctagat
241 ttgactgcaa gattgaagga tacccagacc ccgaggttgt ctggttcaaa gatgaccagt
301 caatcaggga gtcccgccac ttccagatag actacgatga ggacgggaac tgctctttaa
361 ttattagtga tgtttgcggg gatgacgatg ccaagtacac ctgcaaggct gtcaacagtc
421 ttggagaagc cacctgcaca gcagagctca ttgtggaaac gatggaggaa ggtgaagggg
481 aaggggaaga ggaagaagag tgaaacaaag ccagagaaaa gcagtttcta agtcatatta
541 aaaggactat ttctctaaaa ctcaaaaaaa aaaaaaaaac tcaagatagt aaaagcacct
601 agtgtgatag attatcggtt aggtcatttg tgggttgatt cttcagaaac agcagttgat
661 acctagcagc gttattgatg ggcattaatc tatgttagtt ggcaccttaa gatactagtg
721 cagctagatt tcatttaggg aaatcaccag taacttgact gaccaattga ttttagagag
781 aaagtaacca aaccaaatat ttatctgggc aaagtcataa attctccact tgaatgcgct
841 catgaaaaat aaggccaaaa caagagttct gggccacagc tcagcccaga gggttcctgg
901 ggatgggagg cctctctctc cccaccccct gactctagag aactgggttt tctcccagta
961 ctccagcaat tcatttctga aagcagttga gccactttat tccaaagtac actgcagatg
1021 ttcaaactct ccatttctct ttccccttcc acctgccagt tttgctgact ctcaacttgt
1081 catgagtgta agcattaagg acattatgct tcttcgattc tgaagacagg tccctgctca
1141 tggatgactc tggcttcctt aggaaaatat ttttcttcca aaatcagtag gaaatctaaa
1201 cttatcccct ctttgcagat gtctagcagc ttcagacatt tggttaagaa cccatgggaa
1261 aaaaaaaatc cttgctaatg tggtttcctt tgtaaaccag gattcttatt tgtgctgtta
1321 tagaatatca gctctgaacg tgtggtaaag atttttgtgt ttgaatatag gagaaatcag
1381 tttgctgaaa agttagtctt aattatctat tggccacgat gaaacagatt tcaactgata
1441 aagagctgga gaactccatg tactttggaa tctcctccaa gatagccaga gtttaataca
1501 tcttcattct caacactctc caaagaactt gacctacctt atgggttcca tatttttctt
1561 cttaaatgtg catcaatcat gccttgcccc caacctttaa atatattctt agacctggta
1621 aatgcactca gacttgcgtc tttaggaatt tttaactttc tttcactaca ttggcactta
1681 aattttttct ttataaagct ttttgaaggt cataaacaaa gaccataatt gatgatagac
1741 ctaatacatt tcctctgtgt gtgtgtgtaa cattccaaat actttttttt tcttttccac
1801 tgtttgtaag gtgcaacaat ttaatatttt taagggactt tttaagagtt ccttaagaac
1861 caatttaaaa ttacttcagt gcaatcctac acagtatcaa cattagaatt ttgatattag
1921 tcttatgtta tcttccattc tatttttatc tgctttttgc tgctagtttc aaactgccag
1981 tatttttcct tttgctttta aaatagttac aatatttttc atgatagcca cagtattgcc
2041 acagtttatt ataataaagg gtttttattt gatttagcgc attcaaagct tttttctatc
2101 acttttgtgt tcagaatata acctttgtgt gcgtgtatgt tgtgtgtgtg catgtgt
//