LOCUS BC110901 2906 bp mRNA linear HUM 24-JUL-2018
DEFINITION Homo sapiens cullin 2, mRNA (cDNA clone MGC:131970 IMAGE:6161736),
complete cds.
ACCESSION BC110901
VERSION BC110901.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2906)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2906)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-DEC-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
REFERENCE 3 (bases 1 to 2906)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2018) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK Sequence update by database staff to remove vector contamination
COMMENT On Jul 24, 2018 this sequence version replaced BC110901.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 255 Row: m Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19482173.
FEATURES Location/Qualifiers
source 1..2906
/db_xref="H-InvDB:HIT000339791"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:131970 IMAGE:6161736"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2906
/gene="CUL2"
/db_xref="GeneID:8453"
/db_xref="HGNC:HGNC:2552"
/db_xref="IMGT/GENE-DB:2552"
/db_xref="MIM:603135"
CDS 192..2429
/gene="CUL2"
/codon_start=1
/product="CUL2 protein"
/protein_id="AAI10902.1"
/db_xref="GeneID:8453"
/db_xref="HGNC:HGNC:2552"
/db_xref="IMGT/GENE-DB:2552"
/db_xref="MIM:603135"
/translation="MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYA
LCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMD
CLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILI
RMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEY
YKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQ
FLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQEN
MPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLA
KYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMS
MDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQ
IYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKM
NYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMIN
HDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQA
AIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADE
YSYVA"
BASE COUNT 975 a 531 c 604 g 796 t
ORIGIN
1 ggagaccgga gggtgtgtgt tgggcaaagc cggaggcaga ggaggacgat tgttttacgg
61 atcccgtgat cggcccgtcc ttctcccctt tccccccctc cctaccgccc ctgtcccgcc
121 ggggagcggc ggcggccttg gactttgctg tctttcctcg cggagacaga tttcaacact
181 acacttgcac aatgtctttg aaaccaagag tagtagattt tgatgaaaca tggaacaaac
241 ttttgacgac aataaaagcc gtggtcatgt tggaatacgt cgaaagagca acatggaatg
301 accgtttctc agatatctat gctttatgtg tggcctatcc tgaacccctt ggagaaagac
361 tttatacaga aactaagatt tttttggaaa atcatgttcg gcatttgcat aagagagttt
421 tggagtcaga agaacaagta cttgttatgt atcataggta ctgggaagaa tacagcaagg
481 gtgcagacta tatggactgc ttatataggt atctcaacac ccagtttatt aaaaagaata
541 aattaacaga agcggacctt cagtatggct atggtggtgt agatatgaat gaaccactta
601 tggaaatagg agagctagca ttggatatgt ggaggaaatt gatggttgaa ccacttcagg
661 ccatccttat ccgaatgctg ctccgagaaa tcaaaaatga tcgtggtgga gaagacccaa
721 accagaaagt aatccatggg gttattaact cctttgttca tgttgaacag tataagaaaa
781 aattcccctt aaagttttat caggaaattt ttgagtctcc ctttctgact gaaacaggag
841 agtattacaa acaagaagct tcaaatttat tacaagaatc aaactgctca cagtatatgg
901 aaaaggttct aggtagatta aaagatgaag aaattcgatg tcgaaaatac ctacatccaa
961 gttcatatac taaggtgatt catgaatgtc aacaacgaat ggtagcagac cacttacagt
1021 ttttacatgc agaatgtcat aatataattc gacaagagaa aaaaaatgac atggcaaata
1081 tgtacgtctt actccgtgct gtgtccactg gtttacctca tatgattcag gagctgcaaa
1141 accacatcca tgatgagggc cttcgagcaa ccagcaacct tactcaggaa aacatgccaa
1201 cactatttgt ggagtcagtt ttggaagtgc atggtaaatt tgttcagctt atcaacactg
1261 ttttgaatgg tgatcagcat tttatgagtg cgttggataa ggcccttacg tcagttgtaa
1321 attacagaga acctaagtct gtttgcaaag cacctgaact gcttgctaag tactgtgaca
1381 acttactgaa gaagtcagcg aaagggatga cagagaatga agtggaagac aggctcacga
1441 gcttcatcac agtgttcaaa tacattgatg acaaggacgt ctttcaaaag ttctacgcaa
1501 gaatgctggc aaaacgttta attcatgggt tatccatgtc tatggactct gaagaagcca
1561 tgatcaacaa attaaagcaa gcctgtggtt atgagtttac cagcaagcta catcggatgt
1621 atacagatat gagtgtcagc gctgatctca acaataagtt caacaatttt atcaaaaacc
1681 aagacacagt aatagatttg ggaattagtt ttcaaatata tgttctacag gctggtgcgt
1741 ggcctcttac tcaggctcct tcatctacgt ttgcaattcc ccaggaatta gaaaaaagtg
1801 tacagatgtt tgaattattt tatagccaac atttcagtgg aaggaaactt acatggttac
1861 attatctgtg tacaggtgaa gttaaaatga actatttggg caaaccatat gtagccatgg
1921 ttacaacata ccaaatggca gttcttcttg cctttaacaa cagtgaaact gtcagttata
1981 aagagcttca ggacagcact cagatgaatg aaaaggaact gacaaaaaca atcaaatcat
2041 tacttgatgt gaaaatgatt aaccatgatt cagaaaagga agatattgat gcagaatctt
2101 cgttttcatt aaatatgaac tttagcagta aaagaacaaa atttaaaatt actacatcaa
2161 tgcagaaaga cacaccacaa gaaatggagc agactagaag tgcagttgat gaggaccgga
2221 aaatgtatct ccaagctgct atagttcgta tcatgaaagc acgaaaagtg cttcggcaca
2281 atgcccttat tcaagaggtg attagccagt caagagctag gtttaatccc agtatcagca
2341 tgattaagaa gtgtattgaa gttctgatag acaaacaata catagaacgc agccaggcgt
2401 cggcagatga atacagctac gtcgcgtgat gtcgctctcc tccagcgtgg tgtgagaaga
2461 tcattgccat caccatttgg tgtgttcctg tgggaaaaag caggactgtg cctccataat
2521 ttggtcattt ggcagcccct gttttctgct gtttacaaca tcaccagtgc cacgtcatga
2581 gcgtcaaaga aaatgcctag agatatttca agctcatgtc attatgacat ttcttaaaac
2641 tttattaaaa gaatgagtga agtattgctg aaaagtggaa attcggttgg gtaccatgct
2701 ttttctcccc ttcacgtttg cagttgatgt gtcttttttt tttttttaat gtatcttaaa
2761 ggacataaaa tttaaaaact taaatattgt aatatgacag ataacctaat aattgtatct
2821 acattaaaat gacaaacatg atactgctgc ttgtcaaata aaaaaaaaat aaagaaaaaa
2881 aaaaaaaaaa aaaaaaaaaa aaaaaa
//