LOCUS BC108712 2300 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens ceramide kinase, mRNA (cDNA clone IMAGE:6140739),
complete cds.
ACCESSION BC108712
VERSION BC108712.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2300)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2300)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 255 Row: l Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32967302
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2300
/db_xref="H-InvDB:HIT000339116"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6140739"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2300
/gene="CERK"
/gene_synonym="dA59H18.2"
/gene_synonym="dA59H18.3"
/gene_synonym="DKFZp434E0211"
/gene_synonym="FLJ21430"
/gene_synonym="FLJ23239"
/gene_synonym="hCERK"
/gene_synonym="KIAA1646"
/gene_synonym="LK4"
/db_xref="GeneID:64781"
/db_xref="HGNC:HGNC:19256"
CDS 757..1362
/gene="CERK"
/gene_synonym="dA59H18.2"
/gene_synonym="dA59H18.3"
/gene_synonym="DKFZp434E0211"
/gene_synonym="FLJ21430"
/gene_synonym="FLJ23239"
/gene_synonym="hCERK"
/gene_synonym="KIAA1646"
/gene_synonym="LK4"
/codon_start=1
/product="ceramide kinase"
/protein_id="AAI08713.1"
/db_xref="GeneID:64781"
/db_xref="HGNC:HGNC:19256"
/translation="MQRGPASGDPREGPPRQRREGCWGLHTCLITAARLRQSGWEHDL
GVCSGSTDDKLWKLAVCKALASLLLLKCQIPMLYIDAKCLTQPGLGAVRRKLAIRGGG
AGPGLPQDSSEAGPEVCTRGPDLSLSFLHTEFSIFIEHLVLLSQSSVNCVSDVCLLPR
SHDGSLVSSAARGLRRPEDSSRKAFLPRSPGHPSIVYYVLV"
BASE COUNT 524 a 568 c 604 g 604 t
ORIGIN
1 cccacgcgtc cgggacagcg acctcaagga gggggggaag aagcgctttg ggcacatttg
61 cagcagccac ccctcctgct gctgcaccgt ctccaacagc tcctggaact gcgacgggga
121 ggtcctgcac agccctgcca tcgaggtcag agtccactgc cagctggttc gactctttgc
181 acgaggaatt gaagagaatc cgaagccaga ctcacacagc tgagaagccg gcgtcctgct
241 cacaaactgg gaaagtgtga aaactattta agataattat tacagaccaa ttatgttgat
301 atatacattt aaatgtagaa atttattttt gatagttaaa tcttgatttt agaagaaaac
361 ccttttgtca acaattttgt gtacatattt ggcattttca gttctgtacg catctgcggg
421 ttgcagccca cgccgcttac tctcagcgga tgcagctgct cacttggggg cactggcctc
481 ttaggtttta acgatgtcaa cagtgtagtt tagaaaatgg cccgttagtg gctctattgc
541 aataatgtta gggacattat atgatttcca cgcaggtcac accatctggg cctgaggtag
601 cagtgggtca ctttgatcca ctttgcagga cttattctgt aacggtttgt ggccaagttt
661 tgggaagtgg ttgattctct ttgccttcat ttcaccttcc tcttcgttta cggttaggac
721 atcgctgctt gatccttaca atactgtgca actgcaatgc aacgtggccc tgcttcaggt
781 gatccgcggg aggggcctcc acgccagcgc cgggaaggct gctggggcct ccacacctgc
841 ctcatcacgg cggcgaggct acgacaatcc ggctgggagc atgaccttgg cgtctgttct
901 gggagcacgg atgataagct ctggaagctg gcagtgtgta aagcactggc aagtttgtta
961 ctgttaaaat gtcaaatacc aatgctttat atcgacgcga agtgcttaac acagccgggc
1021 ttgggggcag tcaggaggaa gctggccatc cgtggaggag gggccggtcc tggactcccg
1081 caggactcct ctgaggcagg gcctgaagtc tgtacacgtg gtccagattt gtccttgtct
1141 tttcttcaca ctgagttctc tatatttatt gaacatcttg tccttttaag ccagagtagt
1201 gtaaactgcg tctcggatgt ctgtcttttg cctcgaagcc acgatggatc gctggtttcc
1261 tctgcagcgc gagggctccg gcgaccagag gattcttccc ggaaggcatt cctgccgcgc
1321 tccccggggc acccctcaat tgtgtactac gtccttgttt agtgtgtatc cgtgcccacg
1381 tagatgatgt ctgtaacgta gttttgtttg aaatatgaga atatgcggct taaactttga
1441 tctgtaagga gcggggccgt ggccgtttgg agcacgctgt agacaccgtt cctcatgctg
1501 ccgggtgggt tttgcagaag ctcccttagt gatttcatgt ttaacaggca gcatccattt
1561 tcagaatttc ctggcattga tttatatttt gaagcataca ggaaacttct cgtttcctcg
1621 tttagcccca cccagatcag gtgaaagggc agctttaatg gtggttttta tggaccacat
1681 tatcagagag cactgtgcaa gccaaatggt tcaataatga atgaaaattc tgggtgtaaa
1741 gagtaaatat gccctggctc tttctaccaa tgtttgctcc tggttggaaa gaaaccaaag
1801 atttaagacg ggctgctctt ccagactggc tgtgcctgcc tgtgcccagc aacctgtgca
1861 gccggcagtg tgcctggtgt cacgccagga ggctgtggct gctgtgggcc ctctggaatt
1921 gtgctcctca caaagtttcc ccaaaaggtt cttctaagcc tttattgtcc ctggtaaatg
1981 tttcccggct gggcgcggtg gctcacgcct gtaatcccag cactttggga ggccgaggcg
2041 ggtggatcac ctaaggtcag gagtttgaga tcagcctgcc caacatggtg aaacctcgtc
2101 tctactaaaa atacacaact tagccagtct tgttggcgca cgcctgtaat ctcagctact
2161 agggatgctg aggcaggaga atcgcttgaa cccaagaaag aggtggaggt tgcggtgagc
2221 caagattgcg ccactgcact ccagcctggg caaacagagg gagactccat cgcccccccc
2281 caacaaaaaa aaaaaaaaaa
//