LOCUS BC107750 2010 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens cell division cycle 2, G1 to S and G2 to M, mRNA (cDNA
clone MGC:111195 IMAGE:6710672), complete cds.
ACCESSION BC107750
VERSION BC107750.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2010)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2010)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-OCT-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: NCI
cDNA Library Preparation: Michael Brownstein / Ted Usdin
Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 61 Row: m Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 27886643
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..2010
/db_xref="H-InvDB:HIT000338880"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:111195 IMAGE:6710672"
/tissue_type="Pooled, 40 cell lines"
/clone_lib="NIH_MGC_128"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..2010
/gene="CDC2"
/gene_synonym="CDC28A"
/gene_synonym="CDK1"
/db_xref="GeneID:983"
/db_xref="HGNC:HGNC:1722"
/db_xref="MIM:116940"
CDS 104..781
/gene="CDC2"
/gene_synonym="CDC28A"
/gene_synonym="CDK1"
/codon_start=1
/product="CDC2 protein"
/protein_id="AAI07751.1"
/db_xref="GeneID:983"
/db_xref="HGNC:HGNC:1722"
/db_xref="MIM:116940"
/translation="MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGV
PSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDS
SLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVY
THEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFR
NFCFPVF"
BASE COUNT 711 a 305 c 364 g 630 t
ORIGIN
1 gggggctctt ggaaattgag cggagagcga cgcggttgtt gtagctgccg ctgcggccgc
61 cgcggaataa taagccggga tctaccatac ccattgacta actatggaag attataccaa
121 aatagagaaa attggagaag gtacctatgg agttgtgtat aagggtagac acaaaactac
181 aggtcaagtg gtagccatga aaaaaatcag actagaaagt gaagaggaag gggttcctag
241 tactgcaatt cgggaaattt ctctattaaa ggaacttcgt catccaaata tagtcagtct
301 tcaggatgtg cttatgcagg attccaggtt atatctcatc tttgagtttc tttccatgga
361 tctgaagaaa tacttggatt ctatccctcc tggtcagtac atggattctt cacttgttaa
421 gagttattta taccaaatcc tacaggggat tgtgttttgt cactctagaa gagttcttca
481 cagagactta aaacctcaaa atctcttgat tgatgacaaa ggaacaatta aactggctga
541 ttttggcctt gccagagctt ttggaatacc tatcagagta tatacacatg aggtagtaac
601 actctggtac agatctccag aagtattgct ggggtcagct cgttactcaa ctccagttga
661 catttggagt ataggcacca tatttgctga actagcaact aagaaaccac ttttccatgg
721 ggattcagaa attgatcaac tcttcaggat tttcagaaat ttttgttttc ctgtttttta
781 gagctttggg cactcccaat aatgaagtgt ggccagaagt ggaatcttta caggactata
841 agaatacatt tcccaaatgg aaaccaggaa gcctagcatc ccatgtcaaa aacttggatg
901 aaaatggctt ggatttgctc tcgaaaatgt taatctatga tccagccaaa cgaatttctg
961 gcaaaatggc actgaatcat ccatatttta atgatttgga caatcagatt aagaagatgt
1021 agctttctga caaaaagttt ccatatgtta tgtcaacaga tagttgtgtt tttattgtta
1081 actcttgtct atttttgtct tatatatatt tctttgttat caaacttcag ctgtacttcg
1141 tcttctaatt tcaaaaatat aacttaaaaa tgtaaatatt ctatatgaat ttaaatataa
1201 ttctgtaaat gtgtgtaggt ctcactgtaa caactatttg ttactataat aaaactataa
1261 tattgatgtc aggaatcagg aaaaaatttg agttggctta aatcatctca gtccttatgg
1321 cagttttatt ttcctgtagt tggaactact aaaatttagg aaaatgctaa gttcaagttt
1381 cgtaatgctt tgaagtattt ttatgctctg aatgtttaaa tgttctcatc agtttcttgc
1441 catgttgtta actatacaac ctggctaaag atgaatattt ttctactggt attttaattt
1501 ttgacctaaa tgtttaagca ttcggaatga gaaaactata cagatttgag aaatgatgct
1561 aaatttatag gagttttcag taacttaaaa agctaacatg agagcatgcc aaaatttgct
1621 aagtcttaca aagatcaagg gctgtccgca acagggaaga acagttttga aaatttatga
1681 actatcttat ttttaggtag gttttgaaag ctttttgtct aagtgaattc ttatgccttg
1741 gtcagagtaa taactgaagg aggtgcttat cttggctttc gagtctgagt ttaaaactac
1801 acattttgac atagtgttta ttagcagcca tctaaaaagg ctctaatgta tatttaacta
1861 aaattactag ctttgggaat taaactgttt aacaaataaa aaaaaaaaaa aaaaaaaaaa
1921 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
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