LOCUS BC105983 2968 bp mRNA linear HUM 30-JAN-2018
DEFINITION Homo sapiens glutamyl-prolyl-tRNA synthetase, mRNA (cDNA clone
IMAGE:6153037), partial cds.
ACCESSION BC105983
VERSION BC105983.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2968)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2968)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
REFERENCE 3 (bases 1 to 2968)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (30-JAN-2018) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK Sequence update by database staff to remove vector contamination
COMMENT On Jan 30, 2018 this sequence version replaced BC105983.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 232 Row: f Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 62241041.
FEATURES Location/Qualifiers
source 1..2968
/db_xref="H-InvDB:HIT000338350"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6153037"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..>2968
/gene="EPRS"
/gene_synonym="EARS"
/gene_synonym="PARS"
/gene_synonym="PIG32"
/db_xref="GeneID:2058"
/db_xref="HGNC:HGNC:3418"
/db_xref="IMGT/GENE-DB:3418"
/db_xref="MIM:138295"
CDS 44..>2968
/gene="EPRS"
/gene_synonym="EARS"
/gene_synonym="PARS"
/gene_synonym="PIG32"
/codon_start=1
/product="EPRS protein"
/protein_id="AAI05984.1"
/db_xref="GeneID:2058"
/db_xref="HGNC:HGNC:3418"
/db_xref="IMGT/GENE-DB:3418"
/db_xref="MIM:138295"
/translation="MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSE
NVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINEL
NHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAF
QSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAA
LLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETI
MKYAEKLIQEGKAYVDDTPAEQMKAEREQRIESKHRKNPIEKNLQMWEEMKKGSQFGQ
SCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTH
ALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGW
DDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKLRALCKKVID
PVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAET
FSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHAL
PIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRR
GFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFK
ERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEY
KEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAK
INEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDK
VASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKK
KKK"
BASE COUNT 1003 a 546 c 683 g 736 t
ORIGIN
1 acgagccagg cgtactgaca ggtggaccag cggactggtg gagatggcga cgctctctct
61 gaccgtgaat tcaggagacc ctccgctagg agctttgctg gcagtagaac acgtgaaaga
121 cgatgtcagc atttccgttg aagaagggaa agagaatatt cttcatgttt ctgaaaatgt
181 gatattcaca gatgtgaatt ctatacttcg ctacttggct agagttgcaa ctacagctgg
241 gttatatggc tctaatctga tggaacatac tgagattgat cactggttgg agttcagtgc
301 tacaaaatta tcttcatgtg attcctttac ttctacaatt aatgaactca atcattgcct
361 gtctctgaga acatacttag ttggaaactc cttgagttta gcagatttat gtgtttgggc
421 caccctaaaa ggaaatgctg cctggcaaga acagttgaaa cagaagaaag ctccagttca
481 tgtaaaacgt tggtttggct ttcttgaagc ccagcaggcc ttccagtcag taggtaccaa
541 gtgggatgtt tcaacaacca aagctcgagt ggcacctgag aaaaagcaag atgttgggaa
601 atttgttgag cttccaggtg cggagatggg aaaggttacc gtcagatttc ctccagaggc
661 cagtggttac ttacacattg ggcatgcaaa agctgctctt ctgaaccagc actaccaggt
721 taactttaaa gggaaactga tcatgagatt tgatgacaca aatcctgaaa aagaaaagga
781 agattttgag aaggttatct tggaagatgt tgcaatgttg catatcaaac cagatcaatt
841 tacttatact tcggatcatt ttgaaactat aatgaagtat gcagagaagc taattcaaga
901 agggaaggct tatgtggatg atactcctgc tgaacagatg aaagcagaac gtgagcagag
961 gatagaatct aaacatagaa aaaaccctat tgagaagaat ctacaaatgt gggaagaaat
1021 gaaaaaaggg agccagtttg gtcagtcctg ttgtttgcga gcaaaaattg acatgagtag
1081 taacaatgga tgcatgagag atccaaccct ttatcgctgc aaaattcaac cacatccaag
1141 aactggaaat aaatacaatg tttatccaac atatgatttt gcctgcccca tagttgacag
1201 catcgaaggt gttacacatg ccctgagaac aacagaatac catgacagag atgagcagtt
1261 ttactggatt attgaagctt taggcataag aaaaccatat atttgggaat atagtcggct
1321 aaatctcaac aacacagtgc tatccaaaag aaaactcaca tggtttgtca atgaaggact
1381 agtagatgga tgggatgacc caagatttcc tacggttcgt ggtgtactga gaagagggat
1441 gacagttgaa ggactgaaac agtttattgc tgctcagggc tcctcacgtt cagtcgtgaa
1501 catggagtgg gacaaaatct gggcgtttaa caaaaagctg cgagctctct gtaagaaggt
1561 tattgaccca gtggctccac gatatgttgc attactgaag aaagaagtga tcccagtgaa
1621 tgtacctgaa gctcaggagg agatgaaaga agtagccaaa cacccaaaga atcctgaggt
1681 tggcttgaag cctgtgtggt atagtcccaa agttttcatt gaaggtgctg atgcagagac
1741 tttttcggag ggtgagatgg ttacatttat aaattggggc aacctcaaca ttacaaaaat
1801 acacaaaaat gcagatggaa aaatcatatc tcttgatgca aagttgaatt tggaaaacaa
1861 agactacaag aaaaccacta aggtcacttg gcttgcagag actacacatg ctcttcctat
1921 tccagtaatc tgtgtcactt atgagcactt gatcacaaag ccagtgctag gaaaagacga
1981 ggactttaag cagtatgtca acaagaacag taagcatgaa gagctaatgc taggggatcc
2041 ctgccttaag gatttgaaaa aaggagatat tatacaactc cagagaagag gattcttcat
2101 atgtgatcaa ccttatgaac ctgttagccc atatagttgc aaggaagccc cgtgtgtttt
2161 gatatacatt cctgatgggc acacaaagga aatgccaaca tcagggtcaa aggaaaagac
2221 caaagtagaa gccacaaaaa atgagacctc tgctcctttt aaggaaagac caacaccttc
2281 tctgaataat aattgtacta catctgagga ttccttggtc ctttacaata gagtggctgt
2341 tcaaggagat gtggttcgtg aattaaaagc caagaaagca ccaaaggaag atgtagatgc
2401 agctgtaaaa cagcttttgt ctttgaaagc tgaatataag gagaaaactg gccaggaata
2461 taaacctgga aaccctcctg ctgaaatagg acagaatatt tcttctaatt cctcagcaag
2521 tattctggaa agtaaatctc tgtatgatga agttgctgca caaggggagg tggttcgtaa
2581 gctaaaagct gaaaaatccc ctaaggctaa aataaatgaa gctgtagaat gcttactgtc
2641 cctgaaggct cagtataaag aaaaaactgg gaaggagtac atacctggtc agcccccatt
2701 atctcaaagt tcggattcaa gcccaaccag aaattctgaa cctgctggtt tagaaacacc
2761 agaagcgaaa gtactttttg acaaagtagc ttctcaaggg gaagtagttc ggaaacttaa
2821 aactgaaaaa gcccctaagg atcaagtaga tatagctgtt caagaactcc ttcagctaaa
2881 ggcacagtac aagtctttga taggagtaga gtataagcct gtgtcggcca ctggagctga
2941 ggacaaagat aaaaaaaaaa aaaaaaaa
//